scholarly journals The role of large conductance voltage‐ and Ca 2+ ‐activated K + channels as low affinity receptors for 17β‐estradiol in guinea pig urinary bladder smooth muscle excitability

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Aaron Provence ◽  
Kiril Hristov ◽  
Shankar Parajuli ◽  
Georgi Petkov
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Bladder Smooth Muscle ◽  
K Channels ◽  
Urinary Bladder Smooth Muscle ◽  
17Β Estradiol ◽  
Muscle Excitability

Functional Characterization of Adenosine Receptors and Coupling to ATP-Sensitive K+ Channels in Guinea Pig Urinary Bladder Smooth Muscle

2002 ◽  
Vol 300 (3) ◽  
pp. 910-917 ◽  
Author(s):  
Sujatha M. Gopalakrishnan ◽  
Steven A. Buckner ◽  
Ivan Milicic ◽  
Duncan R. Groebe ◽  
Kristi L. Whiteaker ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Adenosine Receptors ◽  
Functional Characterization ◽  
Bladder Smooth Muscle ◽  
K Channels ◽  
Urinary Bladder Smooth Muscle

scholarly journals Constitutive PKA activity is essential for maintaining the excitability and contractility in guinea pig urinary bladder smooth muscle: role of the BK channel

2014 ◽  
Vol 307 (12) ◽  
pp. C1142-C1150 ◽  
Author(s):  
Wenkuan Xin ◽  
Ning Li ◽  
Qiuping Cheng ◽  
Vitor S. Fernandes ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Fluorescence Microscopy ◽  
Guinea Pig ◽  
Bk Channels ◽  
Bk Channel ◽  
Bladder Smooth Muscle ◽  
Ratiometric Fluorescence ◽  
Urinary Bladder Smooth Muscle

The elevation of protein kinase A (PKA) activity activates the large-conductance voltage- and Ca2+-activated K+ (BK) channels in urinary bladder smooth muscle (UBSM) cells and consequently attenuates spontaneous phasic contractions of UBSM. However, the role of constitutive PKA activity in UBSM function has not been studied. Here, we tested the hypothesis that constitutive PKA activity is essential for controlling the excitability and contractility of UBSM. We used patch clamp electrophysiology, line-scanning confocal and ratiometric fluorescence microscopy on freshly isolated guinea pig UBSM cells, and isometric tension recordings on freshly isolated UBSM strips. Pharmacological inhibition of the constitutive PKA activity with H-89 or PKI 14–22 significantly reduced the frequency and amplitude of spontaneous transient BK channel currents (TBKCs) in UBSM cells. Confocal and ratiometric fluorescence microscopy studies revealed that inhibition of constitutive PKA activity with H-89 reduced the frequency and amplitude of the localized Ca2+ sparks but increased global Ca2+ levels and the magnitude of Ca2+ oscillations in UBSM cells. H-89 abolished the spontaneous transient membrane hyperpolarizations and depolarized the membrane potential in UBSM cells. Inhibition of PKA with H-89 or KT-5720 also increased the amplitude and muscle force of UBSM spontaneous phasic contractions. This study reveals the novel concept that constitutive PKA activity is essential for controlling localized Ca2+ signals generated by intracellular Ca2+ stores and cytosolic Ca2+ levels. Furthermore, constitutive PKA activity is critical for mediating the spontaneous TBKCs in UBSM cells, where it plays a key role in regulating spontaneous phasic contractions in UBSM.

scholarly journals IBMX Relaxes Guinea Pig Urinary Bladder Smooth Muscle via Activation of Large Conductance Ca2+-Activated K+ Channels

2012 ◽  
Vol 102 (3) ◽  
pp. 689a
Author(s):  
Wenkuan Xin ◽  
Qiuping Cheng ◽  
Rupal P. Soder ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Bladder Smooth Muscle ◽  
K Channels ◽  
Urinary Bladder Smooth Muscle

scholarly journals Regulation of Guinea Pig Detrusor Smooth Muscle Excitability by 17β-Estradiol: The Role of the Large Conductance Voltage- and Ca2+-Activated K+ Channels

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0141950 ◽  
Author(s):  
Aaron Provence ◽  
Kiril L. Hristov ◽  
Shankar P. Parajuli ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Detrusor Smooth Muscle ◽  
K Channels ◽  
17Β Estradiol ◽  
Muscle Excitability
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