Physiologic Study of Bile Salt and Lipid Secretion in Rats After Liver Transplantation

The effect on sulfobromophathalein transport maximum (Tm) and biliary lipid secretion of sodium glyco-24,25-dihydrofusicate, a micelle-forming compound secreted into bile, has been studied in the hamster and compared to that of a physiological bile salt, sodium taurocholate. Biliary phospholipid and cholesterol secretion increased both during glycodihydrofusidate and taurocholate administration, an observation which suggest that both compounds increased th biliary secretion of micelle-forming compounds. In contrast, only taurocholate increased sulfobromophthalein Tm into bile, while glycodihydrofusidate administration decreased it. This observation suggests that the increase in sulfobromophthalein Tm observed during taurocholate administration is not the result of micellar sequestration. It could rather be the consequence of a specific effect of bile salts on the dye transport system.

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Control of biliary phospholipid secretion. Effect of continuous and discontinuous infusion of taurocholate on biliary phospholipid secretion

Biochemical Journal ◽

10.1042/bj2340421 ◽

1986 ◽

Vol 234 (2) ◽

pp. 421-427 ◽

Cited By ~ 17

Author(s):

K Rahman ◽

T G Hammond ◽

P J Lowe ◽

S G Barnwell ◽

B Clark ◽

...

Keyword(s):

Animal Model ◽

Bile Salt ◽

Biliary Fistula ◽

Perfused Liver ◽

Biliary Lipid ◽

Short Pulses ◽

Lipid Secretion ◽

Canalicular Membrane ◽

Infusion Period ◽

Salt Secretion

A major determinant of biliary lipid secretion is bile-salt secretion. Taurocholate (TC), a micelle-forming bile salt, was infused continuously at different rates in both isolated perfused livers and biliary-fistula rats. In both of these systems, infusion of TC brought about an elevated secretion of phosphatidylcholine for the duration of the TC infusion period. Initial phospholipid/bile-salt ratios in the bile were higher in the whole-animal model than in isolated livers, but at the higher infusion rates both secreted approx. 6 mol of phospholipid for every 100 mol of bile salt. The secretion of phospholipid, which was maintained even at high rates of bile-salt infusion, suggest a continuous and regulated phospholipid supply and secretion mechanism. In contrast, however, multiple short pulses of TC to the perfused liver, which brought about relatively equal biliary bile-salt output pulses, did not bring about equal phospholipid outputs, since the phospholipid peak size declined with each bile-salt pulse. These experiments taken together suggest either that a threshold (intracellular) bile-salt concentration may be required to ‘switch-on’ the phospholipid supply and that it may need to be maintained for continuous biliary phospholipid supply to the canalicular membrane.

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Partial characterization of mechanism(s) by which sulphobromophthalein reduces biliary lipid secretion

Biochemical Journal ◽

10.1042/bj2910173 ◽

1993 ◽

Vol 291 (1) ◽

pp. 173-177 ◽

Cited By ~ 3

Author(s):

G Yamashita ◽

S Tazuma ◽

K Horikawa ◽

N Aihara ◽

H Ochi ◽

...

Keyword(s):

Bile Salt ◽

Sodium Taurocholate ◽

Bile Canaliculus ◽

Biliary Secretion ◽

Dependent Manner ◽

Biliary Lipid ◽

Sprague Dawley ◽

Lipid Secretion ◽

Biliary Lipids ◽

Sprague Dawley Rats

This study was performed to explore the mechanisms by which sulphobromophthalein (BSP) reduces the secretion of biliary lipid using Sprague-Dawley rats (SDR) and mutant rats with congenital conjugated hyperbilirubinaemia bred from SDR (EHBR). We infused the bile-salt-pool-depleted rats with sodium taurocholate at a constant rate of 160 nmol/min per 100 g body wt. with BSP (12.5, 25 and 50 nmol/min per 100 g body wt.) or BSP-GSH (12.5, 25 and 50 nmol/min per 100 g body wt.). The biliary secretion of BSP and BSP-GSH was markedly impaired in EHBR as compared with that in SDR. BSP reduced the biliary secretion of cholesterol and phospholipids in a dose-dependent manner without affecting the secretion of bile salts and composition of fatty acids in phospholipids in SDR, but had no effect on lipid secretion in EHBR. In contrast, BSP-GSH had no such effect on biliary lipids, either in the SDR or EHBR. In addition, the amount of BSP in the liver of EHBR was in the same range as that of SDR. Therefore it is unlikely that an intracellular mechanism is involved in the phenomenon of uncoupling by BSP. We conclude that the uncoupling of biliary lipids from bile-salt secretion by BSP occurs at the level of the bile canaliculus following the secretion of unconjugated BSP.

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Biliary lipid secretion and its control. Effect of taurodehydrocholate

Biochemical Journal ◽

10.1042/bj2450531 ◽

1987 ◽

Vol 245 (2) ◽

pp. 531-536 ◽

Cited By ~ 14

Author(s):

K Rahman ◽

R Coleman

Keyword(s):

Bile Salt ◽

Lipid Vesicles ◽

High Dose ◽

Control Effect ◽

Uniform Size ◽

Short Pulses ◽

Lipid Secretion ◽

Bile Canalicular Membrane ◽

Lag Period ◽

Rat Livers

Multiple short pulses of taurocholate (TC) brought about, in isolated perfused rat livers, the secretion of phospholipid and cholesterol into bile; the lipids showed an appreciable lag period behind the bile-salt secretion, and there was considerable variability in response, both between low and high dose pulses of TC and, at the higher dose, even between individual livers. When a background continuous infusion of taurodehydrocholate (a hydrophilic non-micelle-forming bile-salt analogue) was superimposed upon the short TC pulses, the lipid secretion showed much better control, and the lipid peaks were of more uniform size, following more closely, or more coincident with, the bile-salt output peaks. Taurodehydrocholate may provide a signal for the control of the supply and delivery of lipid vesicles to the bile-canalicular membrane, from where the lipid vesicles are then removed by the action of the pulses of TC.

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Rituximab as therapy for the recurrence of bile salt export pump deficiency after liver transplantation

Liver Transplantation ◽

10.1002/lt.23754 ◽

2013 ◽

Vol 19 (12) ◽

pp. 1403-1410 ◽

Cited By ~ 25

Author(s):

Henry C. Lin ◽

Luis Alvarez ◽

Greggy Laroche ◽

Hector Melin-Aldana ◽

Kim Pfeifer ◽

...

Keyword(s):

Liver Transplantation ◽

Bile Salt ◽

Bile Salt Export Pump

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Effects of sulfobromophthalein excretion on biliary lipid secretion in humans and dogs

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1981.240.1.g85 ◽

1981 ◽

Vol 240 (1) ◽

pp. G85-G89

Author(s):

E. A. Shafter ◽

R. M. Preshaw

Keyword(s):

Bile Salt ◽

Lipid Composition ◽

Control Period ◽

Physical Interaction ◽

Biliary Lipid ◽

Lipid Secretion ◽

Maximal Output ◽

Biliary Lipid Composition ◽

Molar Percent ◽

Salt Secretion

The effect of sulfobromophthalein (BSP) on biliary lipid secretion was investigated in 12 cholecystectomized subjects, using a duodenal marker-perfusion technique. A 1-h basal period was followed by intravenous BSP infusion over 3 h, achieving the maximal excretory rate (Tm). The calculated Tm was not different from the measured maximal output. At BSP Tm, bile salt secretion was unchanged, but phospholipid, cholesterol, and bilirubin secretion were markedly reduced. Biliary lipid composition changed accordingly, higher molar percent bile salts but lower phospholipid and cholesterol. In six cholecystectomized dogs with chronic duodenal fistulas, bile was collected directly from the common duct while bile salt secretion was maintained by intravenous taurocholic acid infusion. After a 2-h control period, sufficient BSP was added to create either maximal (Tm) or submaximal conditions. BSP did not alter bile salt secretion but caused a dose-related decrease in phospholipid and cholesterol secretion. Bilirubin excretion was also reduced, whereas bile flow increased. Thus, BSP is hydrocholeretic but decreases phospholipid, cholesterol, and bilirubin secretion in both humans and dogs. The effect on biliary lipid composition is probably through a physical interaction with biliary micelles.

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