T cell receptor Vβ repertoire of the antigen specific CD8 T lymphocyte subset of HIV infected children

AIDS ◽  
2002 ◽  
Vol 16 (11) ◽  
pp. 1459-1465 ◽  
Author(s):  
Thomas W. McCloskey ◽  
Viraga Haridas ◽  
Rajendra Pahwa ◽  
Savita Pahwa
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
T Lymphocyte ◽  
Cell Receptor ◽  
Lymphocyte Subset ◽  
Cd8 T Lymphocyte ◽  
T Cell Receptor Vβ ◽  
T Lymphocyte Subset

scholarly journals T-cell receptor Vβ repertoire of CD8+ T-lymphocyte subpopulations in cutaneous leishmaniasis patients from the state of Rio de Janeiro, Brazil

2015 ◽  
Vol 110 (5) ◽  
pp. 596-605 ◽  
Author(s):  
Raquel Ferraz ◽  
Clarissa Ferreira Cunha ◽  
Maria Inês Pimentel ◽  
Marcelo Rosandiski Lyra ◽  
Armando Oliveira Schubach ◽  
...  
Keyword(s):  
T Cell ◽  
Cutaneous Leishmaniasis ◽  
T Cell Receptor ◽  
T Lymphocyte ◽  
Cell Receptor ◽  
Lymphocyte Subpopulations ◽  
The State ◽  
T Lymphocyte Subpopulations ◽  
Cd8 T Lymphocyte ◽  
T Cell Receptor Vβ

scholarly journals Human CD8+ T lymphocyte clones specific for T cell receptor Vβ families expressed on autologous CD4+ T cells

Immunity ◽  
1995 ◽  
Vol 2 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Randle Ware ◽  
Hong Jiang ◽  
Ned Braunstein ◽  
Jennifer Kent ◽  
Ethan Wiener ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
T Lymphocyte ◽  
Cd4 T Cells ◽  
Cell Receptor ◽  
Cd8 T Lymphocyte ◽  
T Cell Receptor Vβ

T-cell receptor usage in T lymphocyte clones specifically recognizing human melanoma

1993 ◽  
Vol 3 (1) ◽  
pp. 26
Author(s):  
M. Sensi ◽  
S. Salvi ◽  
C. Castelli ◽  
G. Nicolini ◽  
A. Anichini ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
T Lymphocyte ◽  
Cell Receptor ◽  
Human Melanoma ◽  
Receptor Usage

Selection for T-cell receptor Vβ–Dβ–Jβ gene rearrangements with specificity for a myelin basic protein peptide in brain lesions of multiple sclerosis

Nature ◽  
1993 ◽  
Vol 362 (6415) ◽  
pp. 68-70 ◽  
Author(s):  
Jorge R. Oksenberg ◽  
Michael A. Panzara ◽  
Ann B. Begovich ◽  
Dennis Mitchell ◽  
Henry A. Erlich ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cell ◽  
Myelin Basic Protein ◽  
T Cell Receptor ◽  
Basic Protein ◽  
Cell Receptor ◽  
Brain Lesions ◽  
Gene Rearrangements ◽  
Selection For ◽  
T Cell Receptor Vβ

scholarly journals Simplified Fluorescent Multiplex PCR Method for Evaluation of the T-Cell Receptor Vβ-Chain Repertoire

2005 ◽  
Vol 12 (4) ◽  
pp. 477-483 ◽  
Author(s):  
Sanjit Fernandes ◽  
Surendra Chavan ◽  
Vivek Chitnis ◽  
Nina Kohn ◽  
Savita Pahwa
Keyword(s):  
T Cell ◽  
Multiplex Pcr ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
Clinical Samples ◽  
Cdr3 Length ◽  
Pcr Products ◽  
Pcr Method ◽  
T Cell Receptor Vβ

ABSTRACTRationale: evaluation of the T-cell receptor (TCR) Vβ-chain repertoire by PCR-based CDR3 length analysis allows fine resolution of the usage of the TCR Vβ repertoire and is a sensitive tool to monitor changes in the T-cell compartment. A multiplex PCR method employing 24 labeled upstream Vβ primers instead of the conventionally labeled downstream Cβ primer is described. Method: RNA was isolated from purified CD4 and CD8 T-cell subsets from umbilical cord blood and clinical samples using TRI reagent followed by reverse transcription using a Cβ primer and an Omniscript RT kit. The 24 Vβ primers were multiplexed based on compatibility and product sizes into seven reactions. cDNA was amplified using 24 Vβ primers (labeled with tetrachloro-6-cardoxyfluorescein, 6-carboxyfluorescein, and hexachloro-6-carboxyfluorescein), an unlabeled Cβ primer, and Taqgold polymerase. The fluorescent PCR products were resolved on an automated DNA sequencer and analyzed using the Genotyper 2.1 software. Results: Vβ spectratypes of excellent resolution were obtained with RNA amounts of 250 ng using the labeled Vβ primers. The resolution was superior to that obtained with the labeled Cβ primer assay. Also the numbers of PCRs were reduced to 7 from the 12 required in the Cβ labeling method, and the sample processing time was reduced by half. Conclusion: The method described for T-cell receptor Vβ-chain repertoire analysis eliminates tedious dilutions and results in superior resolution with small amounts of RNA. The fast throughput makes this method suitable for automation and offers the feasibility to perform TCR Vβ repertoire analyses in clinical trials.

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