CHARACTERIZATION OF T CELL RECEPTORS UTILIZED BY A HUMAN TUMOR SPECIFIC CYTOTOXIC T LYMPHOCYTE LINE

Antigen-binding receptors on T lymphocytes and IgG antibodies with the same antigen-binding specificity as the T-cell receptors display shared or identical idiotypes. This was shown using a system where adult F1 hybrid rats between two inbred strains were inoculated with T lymphocytes from one parental strain. Such F1 hybrid rats produce antibodies directed against idiotypic determinants present on IgG alloantibodies, produced in the T donor genotype strain and with specificity for the alloantigens of the other parental strain. The idiotypic nature of the F1 antialloantibody serum against the parental alloantibodies was demonstrated both by indirect hemagglutination tests or by gel diffusion using alloantisera with different specificity as targets. Furthermore, the F1 anti-T-lymphocyte sera could be shown to contain antibodies against idiotypic parental T lymphocytes as well. This was shown by the capacity of the antisera, in the presence of complement, to wipe out the relevant parental T-cell reactivity against the other parental strain (as measured in MLC or GVH) whilst leaving the T-lymphocyte reactivity against a third, unrelated allogeneic strain intact. These findings demonstrate that F1 hybrid rats inoculated with parental T lymphocytes make anti-idiotypic antibodies directed against both the T cell receptors and IgG alloantibodies of that parental strain with specificity for alloantigens of the other parental strain. In order to prove identity between the anti-idiotypic antibodies against the B and T-cell antigen-binding molecules the following experiments were carried out; highly purified IgG from relevant alloantibody-containing serum in immunosorbent from could be shown to selectively remove both anti-idiotypic activities from the F1 antiserum. Further more, parental normal T lymphocytes could be shown capable of removing from the anti-idiotypic antisera all those antibodies that would cause agglutination of the relevant alloantibody-coated erythrocytes in the indirect agglutination assay. We would thus conclude that T and B lymphocytes reactive against a given antigenic determinant use receptors with antigen-binding areas coded for by the same variable gene subset(s).

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Immunoglobulins, MHC and T Cell receptors genes in Cetaceans

10.1101/2020.10.24.353342 ◽

2020 ◽

Author(s):

Francisco Gambón-Deza

Keyword(s):

T Cell ◽

Mhc Class I ◽

Marine Environment ◽

T Lymphocyte ◽

T Cell Receptors ◽

Immunoglobulin M ◽

Class I ◽

Cell Receptors ◽

Vh Genes ◽

Lymphocyte Receptors

AbstractCetaceans correspond to mammals that have returned to the marine environment. Adaptive changes are very significant with the conversion of the limbs into flippers. It is studied the changes that have occurred in immunoglobulins, MHC class I and II and T cell receptors genes. Constant regions of immunoglobulins are similar to those of the rest of mammals. An exception is the IgD gene, which is composed of three CH domains but CH1 similar to CH1 of immunoglobulin M. In the IGHV locus, it exist a decrease in the number of VH genes with the absence of genes within Clan I. The number of Vλ genes is greater than that of Vκ. In the genes for T lymphocyte receptors, it exists a decrease in the number of Vα genes with loss of significant clades and subclades. In Vβ and Vγ, there is also the loss of clades. These declines of Vα, Vβ and Vγ are not present Artiodactyla, and they are specific to Cetaceans. In MHC present tree evolutive lines of class I genes. These species have DQ, DR, DO and DM genes, but they are no present DP genes.

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Identification and characterization of rat T cell subpopulations expressing T cell receptors α/β and γ/δ

European Journal of Immunology ◽

10.1002/eji.1830200217 ◽

1990 ◽

Vol 20 (2) ◽

pp. 343-349 ◽

Cited By ~ 40

Author(s):

Alexandra Lawetzky ◽

Georg Tiefenthaler ◽

Ralph Kubo ◽

Thomas Hünig

Keyword(s):

T Cell ◽

T Cell Receptors ◽

Cell Receptors ◽

Cell Subpopulations ◽

T Cell Subpopulations ◽

Identification And Characterization

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Cloning and Expression of Human Membrane-Bound and Soluble Engineered T Cell Receptors for Immunotherapy

Journal of Biomedicine and Biotechnology ◽

10.1155/jbb/2006/68091 ◽

2006 ◽

Vol 2006 ◽

pp. 1-9

Author(s):

Nehad M. Alajez ◽

Saman Eghtesad ◽

Olivera J. Finn

Keyword(s):

T Cells ◽

T Cell ◽

T Cell Receptors ◽

Mammalian Cells ◽

Human Tumor ◽

Cloning And Expression ◽

Single Chain ◽

Cell Receptors ◽

Mammalian Expression ◽

Membrane Bound

We report here the design and construction of several gene vectors for expression in mammalian cells of membrane-bound and soluble human T cell receptors (TR). We designed a vector (TR-ALPHA-IRES-TR-BETA pEF4) that encodes high-level expression of the full-length TR on the surface of T cells. Furthermore, we engineered TR that does not require the presence of endogenous CD3 molecules for surface expression and thus expression is not limited to T cells. We also constructed a vector encoding a single-chain TR (scTR) as a fusion protein of V-ALPHA-V-BETA-C-BETA with CD3Z. Since it is encoded and expressed as a single molecule, this scTR is well suited for gene therapy. Lastly, we successfully used a mammalian expression vector for generation of soluble human TR. The approaches we used here for manipulation of a human tumor-specific TR can be useful for other investigators interested in TR-based immunotherapy.

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