Engineering and Characterization of a Novel Fusion Protein Incorporating B7.2 and an Anti–ErbB-2 Single-Chain Antibody Fragment for the Activation of Jurkat T Cells

2001 ◽  
Vol 24 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Keith W. Marshall ◽  
James D. Marks
Keyword(s):  
T Cells ◽  
Fusion Protein ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Jurkat T Cells ◽  
Single Chain Antibody Fragment

T-cell activation and cytokine production via a bispecific single-chain antibody fragment targeted to blood-stage malaria parasites

Blood ◽  
2003 ◽  
Vol 101 (6) ◽  
pp. 2300-2306 ◽  
Author(s):  
Shigeto Yoshida ◽  
Tominari Kobayashi ◽  
Hiroyuki Matsuoka ◽  
Chisato Seki ◽  
William L. Gosnell ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell Activation ◽  
Cell Activation ◽  
Recombinant Baculovirus ◽  
Antibody Fragment ◽  
Antibody Fragments ◽  
Therapeutic Drug ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Single Chain Antibody Fragment

A novel bispecific single-chain antibody fragment (biscFv) has been constructed to address the possibility of a new approach to malaria therapeutic drug development. The biscFv consists of 2 different single-chain antibody fragments linked by a flexible peptide linker (Gly4-Ser)3. Of the 2 scFv fragments, one is directed against a conserved epitope of the 19-kDa C-terminal fragment of the major surface protein of human malignant malaria parasite, Plasmodium falciparum, and the other is directed against the CD3 antigen of human T cells. The biscFv expressed by a recombinant baculovirus retained the antigen-binding properties of the corresponding univalent single-chain antibody fragments and formed a bridge between P falciparum and T cells. In cooperation with T cells, the biscFv specifically induced not only interferon γ and tumor necrosis factor α, but also a significant increase of merozoite phagocytosis and growth inhibition of P falciparum in vitro. Thus, the biscFv possesses highly selective malaria-targeting properties and stimulates T cells to induce cytokines, presumably resulting in activation of macrophages, neutrophils, and natural killer cells, and parasite killing in vivo.

Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment

2012 ◽  
Vol 522 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Francesca Paoletti ◽  
Francesca Malerba ◽  
Petr V. Konarev ◽  
Michela Visintin ◽  
Raffaella Scardigli ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Single Chain Antibody Fragment

Bacterial expression and characterization of an anti-desipramine single-chain antibody fragment

1996 ◽  
Vol 18 (12) ◽  
pp. 729-738 ◽  
Author(s):  
Gaofeng Lin ◽  
Paul R. Pentel ◽  
Weilin L. Shelver ◽  
Daniel E. Keyler ◽  
Catherine A. Ross ◽  
...  
Keyword(s):  
Antibody Fragment ◽  
Bacterial Expression ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Single Chain Antibody Fragment

scholarly journals Bacterial expression and characterization of an anti-CD22 single-chain antibody fragment

2020 ◽  
Vol 170 ◽  
pp. 105594
Author(s):  
Diana Mikiewicz ◽  
Natalia Łukasiewicz ◽  
Marcin Zieliński ◽  
Violetta Cecuda-Adamczewska ◽  
Anna Bierczyńska-Krzysik ◽  
...  
Keyword(s):  
Antibody Fragment ◽  
Bacterial Expression ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Single Chain Antibody Fragment

Generation and Characterization of a Mouse Single-Chain Antibody Fragment Specific for Disialoganglioside (GD2)

Hybridoma ◽  
1997 ◽  
Vol 16 (4) ◽  
pp. 335-346 ◽  
Author(s):  
SANDRINE MOUTEL ◽  
STEPHANE BIRKLE ◽  
VALERIE LAURENCE ◽  
JEAN MICHON ◽  
WOLF HERMAN FRIDMAN ◽  
...  
Keyword(s):  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Single Chain Antibody Fragment
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