Improved Human Keratinocyte Growth on Acellular Dermal Matrix In Vitro

1998 ◽  
Vol 19 ◽  
pp. S171 ◽  
Author(s):  
L J Jennings ◽  
M L Hanumadass ◽  
S Sethi ◽  
R J Walter
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Human Keratinocyte ◽  
Acellular Dermal

Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix

2009 ◽  
Vol 610-613 ◽  
pp. 1298-1301
Author(s):  
Xiang Rong Zhang ◽  
De Wu Liu ◽  
Guang Hua Guo ◽  
Yan Peng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gradient Centrifugation ◽  
Human Mesenchymal Stem Cells ◽  
Acellular Dermal Matrix ◽  
Culture Method ◽  
Gene Encoding ◽  
Dermal Matrix ◽  
Acellular Dermal

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.

scholarly journals Three-dimensional cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds and their biocompatibility evaluation in vitro

RSC Advances ◽  
2020 ◽  
Vol 10 (27) ◽  
pp. 15926-15935 ◽  
Author(s):  
Peng Liu ◽  
Qing Li ◽  
Qiaolin Yang ◽  
Shihan Zhang ◽  
Chunping Lin ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Three Dimensional ◽  
Acellular Dermal Matrix ◽  
Biological Properties ◽  
Gingival Fibroblast ◽  
Dermal Matrix ◽  
Cell Printing ◽  
Acellular Dermal ◽  
Dimensional Cell

3D cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds showed satisfactory biological properties.

scholarly journals In Vitro Analysis of Histology, Mechanics, and Safety of Radiation-free Pre-hydrated Human Acellular Dermal Matrix

2020 ◽  
Vol 23 (6) ◽  
pp. 635
Author(s):  
Ji Young Kim ◽  
Kyung Min Yang ◽  
Ji Hyun Youn ◽  
Heejun Park ◽  
Hyung Min Hahn ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Vitro Analysis ◽  
Acellular Dermal ◽  
Human Acellular Dermal Matrix ◽  
In Vitro Analysis

An Improved Method for the Preparation for New Antibacterial Dressing Crosslinked with Carboxymethyl Chitosan

2018 ◽  
Vol 1145 ◽  
pp. 39-43
Author(s):  
Qian Wang ◽  
Ke Gong ◽  
Cheng Bo Cao ◽  
Jing Guo ◽  
Xiao Jiao Wang
Keyword(s):  
Acellular Dermal Matrix ◽  
Carboxymethyl Chitosan ◽  
Inhibition Rate ◽  
Improved Method ◽  
Dermal Matrix ◽  
Accelerate Wound Healing ◽  
Time Scanning ◽  
Acellular Dermal ◽  
Degradation Time

A new antibacterial acellular dermal matrix (AADM) dressing can not only resist infection, but also accelerate wound healing. AADM was prepared by SDS (0.40%), trypsin (0.40%) and keratin (0.30%), and then cross-linked with carboxymethyl chitosan (1%) with glutaraldehyde, whose tensile strength (MPa) is 10.66, thickness (mm), 0.53, the rate of permeable steam (g • m-2 • 24h-1), 3640, porosity (%), 81, degradation time in vitro (h), 24.33, pH, 6.5, and the average inhibition rate, more than 70%. At the same time, scanning electron microscopy showed that the structure of AADM was evacuated and the pores were interconnected. On such basis, a conclusion was drawn: the properties of AADM has been sharply increased, compared with acellular dermal matrix, which cannot resist infection, and the new antibacterial acellular dermal matrix completely meets the clinical requirements for burn dressings.

Construction of a dermis–fat composite in vivo: Optimizing heterogeneous acellular dermal matrix with in vitro pretreatment

2019 ◽  
Vol 14 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Zhu Zhu ◽  
Zhao‐Qi Yuan ◽  
Cheng Huang ◽  
Rui Jin ◽  
Di Sun ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Acellular Dermal

scholarly journals Biocompatibility Evaluation of Human and Porcine Acellular Dermal Matrix on Human Primary Gingival Fibroblasts: In Vitro Comparative Study

2021 ◽  
Author(s):  
Ehab Azab ◽  
Abdel-Rahman Youssef
Keyword(s):  
Cell Viability ◽  
Acellular Dermal Matrix ◽  
P Value ◽  
Gingival Fibroblasts ◽  
Dermal Matrix ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

Abstract Objective Allogeneic and xenogeneic acellular dermal matrix (ADM) grafts have been used to treat periodontal soft tissue defects. The purpose of the current study was to compare the effect of human ADM (AlloDerm) and porcine ADM (Derma) on human primary gingival fibroblasts in vitro regarding the biocompatibility test. Materials and Methods Gingival fibroblasts were obtained from healthy adult gingiva and seeded on AlloDerm or Derma ADM in 96-well plate. The control cells were grown on a surface-treated polystyrene cell-culture plate without matrix. The cells were cultured for 3, 7, and 14 days. The fibroblasts morphology was examined using inverted microscopy, and the cell viability of fibroblasts adherent to the dermal matrix was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay after 3, 7, and 14 days in culture. The data were statistically evaluated by one-way analysis of variance. p-Value of 0.05 was considered significant. Results Gingival fibroblasts adjacent to the AlloDerm and Derma matrices were healthy, attached to the well, and did not exhibit any cytopathic changes similar to control. There were no statistically significant differences in the cell viability between the gingival fibroblasts attached to Derma and AlloDerm on day 3 (p = 0.841), day 7 (p = 0.198), and day 14 (p = 0.788). Conclusion Considering this in vitro study’s limitations, both human and porcine ADM were compatible with the surrounding human primary gingival fibroblasts. No significant differences were observed in the cell viability between the gingival fibroblasts that were attached to Derma and AlloDerm.

In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures

2013 ◽  
Vol 49 (3) ◽  
pp. 371-381 ◽  
Author(s):  
A. M. Pabst ◽  
A. Happe ◽  
A. Callaway ◽  
T. Ziebart ◽  
S. I. Stratul ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Porcine Acellular Dermal Matrix ◽  
In Vivo Characterization ◽  
Acellular Dermal
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