Three-dimensional cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds and their biocompatibility evaluation in vitro

The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.

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Effect of In Vitro Gingival Fibroblast Seeding on the In Vivo Incorporation of Acellular Dermal Matrix Allografts in Dogs

Journal of Periodontology ◽

10.1902/jop.2007.060060 ◽

2007 ◽

Vol 78 (2) ◽

pp. 296-303 ◽

Cited By ~ 26

Author(s):

Arthur B. Novaes ◽

Julie Teresa Marchesan ◽

Guilherme O. Macedo ◽

Daniela B. Palioto

Keyword(s):

Acellular Dermal Matrix ◽

Gingival Fibroblast ◽

Dermal Matrix ◽

Acellular Dermal

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Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix

Materials Science Forum ◽

10.4028/www.scientific.net/msf.610-613.1298 ◽

2009 ◽

Vol 610-613 ◽

pp. 1298-1301

Author(s):

Xiang Rong Zhang ◽

De Wu Liu ◽

Guang Hua Guo ◽

Yan Peng

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Gradient Centrifugation ◽

Human Mesenchymal Stem Cells ◽

Acellular Dermal Matrix ◽

Culture Method ◽

Gene Encoding ◽

Dermal Matrix ◽

Acellular Dermal

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.

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Three-Dimensional Bioprinting of a Full-Thickness Functional Skin Model Using Acellular Dermal Matrix and Gelatine Methacrylamide Bioink

SSRN Electronic Journal ◽

10.2139/ssrn.3814585 ◽

2021 ◽

Author(s):

Ronghua Jin ◽

Yuecheng Cui ◽

Haojiao Chen ◽

Zhenzhen Zhang ◽

Tingting Weng ◽

...

Keyword(s):

Three Dimensional ◽

Acellular Dermal Matrix ◽

Full Thickness ◽

Skin Model ◽

Dermal Matrix ◽

Acellular Dermal

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In vitro Biomimetic Construction of Hydroxyapatite–Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture

Tissue Engineering Part A ◽

10.1089/ten.tea.2010.0196 ◽

2011 ◽

Vol 17 (5-6) ◽

pp. 765-776 ◽

Cited By ~ 25

Author(s):

Hongshi Zhao ◽

Guancong Wang ◽

Shunpeng Hu ◽

Jingjie Cui ◽

Na Ren ◽

...

Keyword(s):

Matrix Composite ◽

Composite Scaffold ◽

Acellular Dermal Matrix ◽

Dermal Matrix ◽

Porcine Acellular Dermal Matrix ◽

Acellular Dermal

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In Vitro Analysis of Histology, Mechanics, and Safety of Radiation-free Pre-hydrated Human Acellular Dermal Matrix

Journal of Breast Cancer ◽

10.4048/jbc.2020.23.e64 ◽

2020 ◽

Vol 23 (6) ◽

pp. 635

Author(s):

Ji Young Kim ◽

Kyung Min Yang ◽

Ji Hyun Youn ◽

Heejun Park ◽

Hyung Min Hahn ◽

...

Keyword(s):

Acellular Dermal Matrix ◽

Dermal Matrix ◽

Vitro Analysis ◽

Acellular Dermal ◽

Human Acellular Dermal Matrix ◽

In Vitro Analysis

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An Improved Method for the Preparation for New Antibacterial Dressing Crosslinked with Carboxymethyl Chitosan

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1145.39 ◽

2018 ◽

Vol 1145 ◽

pp. 39-43

Author(s):

Qian Wang ◽

Ke Gong ◽

Cheng Bo Cao ◽

Jing Guo ◽

Xiao Jiao Wang

Keyword(s):

Acellular Dermal Matrix ◽

Carboxymethyl Chitosan ◽

Inhibition Rate ◽

Improved Method ◽

Dermal Matrix ◽

Accelerate Wound Healing ◽

Time Scanning ◽

Acellular Dermal ◽

Degradation Time

A new antibacterial acellular dermal matrix (AADM) dressing can not only resist infection, but also accelerate wound healing. AADM was prepared by SDS (0.40%), trypsin (0.40%) and keratin (0.30%), and then cross-linked with carboxymethyl chitosan (1%) with glutaraldehyde, whose tensile strength (MPa) is 10.66, thickness (mm), 0.53, the rate of permeable steam (g • m-2 • 24h-1), 3640, porosity (%), 81, degradation time in vitro (h), 24.33, pH, 6.5, and the average inhibition rate, more than 70%. At the same time, scanning electron microscopy showed that the structure of AADM was evacuated and the pores were interconnected. On such basis, a conclusion was drawn: the properties of AADM has been sharply increased, compared with acellular dermal matrix, which cannot resist infection, and the new antibacterial acellular dermal matrix completely meets the clinical requirements for burn dressings.

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