Plasma Assay of Salbutamol by Means of High-Performance Liquid Chromatography with Amperometric Determination Using a Loop Column for Injection of Plasma Extracts. Application to the Evaluation of Subcutaneous Administration of Salbutamol

1992 ◽  
Vol 14 (3) ◽  
pp. 243-248 ◽  
Author(s):  
L. Tamisier-Karolak ◽  
B. Delhotal-Landes ◽  
P. Jolliet-Riant ◽  
J. Milliez ◽  
D. Jannet ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Subcutaneous Administration ◽  
Amperometric Determination ◽  
Plasma Assay

scholarly journals Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Faithful Makita-Chingombe ◽  
Anthony T. Podany ◽  
Timothy Mykris ◽  
Farai Muzambi ◽  
Richard W. Browne ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cross Validation ◽  
Medical Center ◽  
Resource Limited ◽  
Aids Clinical Trials ◽  
The University ◽  
University Of Zimbabwe ◽  
Plasma Assay

An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

scholarly journals Separation and Quantification of Eight Antidiabetic Drugs on A High-Performance Liquid Chromatography: Its Application to Human Plasma Assay

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Karunanidhi S. Lakshmi ◽  
Tirumala Rajesh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Solid Phase ◽  
Internal Standard ◽  
Antidiabetic Drugs ◽  
Standard Analysis ◽  
Interday Precision ◽  
Plasma Assay

An analytical method based on isocratic reverse phase high-performance liquid chromatography was developed and validated for the separation and quantification of eight antidiabetic drugs: rosiglitazone, pioglitazone, glipizide, gliclazide, repaglinide, nateglinide, glibenclamide, and glimepiride for their application in human plasma assay. Metformin is used as internal standard. Analysis was done on Onyx monolithic C18 column (100×4.6 mm, i.d., 5 μm) using a mixture of 0.05% formic acid in water and methanol in the ratio of 42 : 58 (v/v) fixed at a flow rate of 0.5 mL/min, and they were monitored at 234 nm. Separation was achieved in less than 20 min. The calibration curves were linear in the range of 50–2000 ng/mL. The method was validated for its recovery, intra- and interday precision, stability, specificity, and selectivity. Plasma samples were prepared using solid-phase extraction of analytes. Hence, the developed method was found to be suitable for the routine analysis of selected antidiabetic drugs in biological matrices.

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