Immunohistochemical diagnosis of malignant melanoma of the conjunctiva and uvea: comparison of the novel antibody against melan-A with S100 protein and HMB-45

Abstract Blue nevus is one of the melanotic lesions that can incidentally arise in the prostate gland. A literature review identified 28 previously reported cases, and although rare, the blue nevus appeared to be the commonest melanocytic lesion arising in the prostate. The differential diagnosis includes melanosis and malignant melanoma, as well as nonmelanotic lesions due to deposition of lipofuscin, hemosiderin and, rarely, homogentisic acid. The distinction among these lesions can typically be made based on morphologic grounds but may also be aided by histochemical and immunohistochemical stains such as stains for iron, S100 protein, HMB-45, and CD68 as needed. Blue nevus of the prostate is a benign lesion with no malignant potential to date, so no further treatment is warranted.

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A Case of Primary Subglottic Malignant Melanoma with a Successful Surgical Treatment

Case Reports in Oncological Medicine ◽

10.1155/2014/968926 ◽

2014 ◽

Vol 2014 ◽

pp. 1-3

Author(s):

Shahzad Ahmad ◽

Mahmoud Abdelghany ◽

Curtis Goldblatt ◽

Owen Stark ◽

Nicholas Masciotra

Keyword(s):

Surgical Treatment ◽

Malignant Melanoma ◽

Excisional Biopsy ◽

Human Melanoma ◽

Primary Malignant Melanoma ◽

Shortness Of Breath ◽

S 100 ◽

Hmb 45

Primary subglottic malignant melanoma is a very rare and underdiagnosed neoplasm. We are reporting a case of primary malignant melanoma of subglottic mucosa in a 78-year-old woman who presented to our hospital with shortness of breath and hoarseness of voice. Laryngoscopy and excisional biopsy along with immunoreactivity to S-100 and human melanoma black-45 (HMB-45) confirmed the diagnosis. The patient was treated with laryngectomy followed by radiotherapy. Five years following surgical treatment, she continues to be asymptomatic. To our knowledge, there is only one reported case of primary malignant melanoma of subglottic mucosa in the medical literatures.

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Tyrosinase Expression in Malignant Melanoma, Desmoplastic Melanoma, and Peripheral Nerve Tumors

Archives of Pathology & Laboratory Medicine ◽

10.5858/2002-126-0816-teimmd ◽

2002 ◽

Vol 126 (7) ◽

pp. 816-822 ◽

Cited By ~ 6

Author(s):

Jenny L. Boyle ◽

Helen M. Haupt ◽

Jere B. Stern ◽

Hinke A. B. Multhaupt

Keyword(s):

Peripheral Nerve ◽

S100 Protein ◽

Dermatofibrosarcoma Protuberans ◽

Antigen Retrieval ◽

Nerve Sheath Tumors ◽

Peripheral Nerve Sheath Tumors ◽

Desmoplastic Melanoma ◽

Nerve Sheath ◽

Tyrosinase Expression ◽

Hmb 45

Abstract Context.—Pathologists may encounter problems in the differential diagnosis of malignant melanoma, spindle and epithelioid neoplasms of peripheral nerves, and fibrohistiocytic tumors. Tyrosinase has been demonstrated to be a sensitive marker for melanoma. Objective.—To determine the specificity of tyrosinase expression in the differential diagnosis of melanoma, desmoplastic melanoma, and peripheral nerve sheath tumors. Design.—Immunoreactivity for tyrosinase, HMB-45 (anti-gp100 protein), S100 protein, CD34, and vimentin was studied in 70 tumors, including 15 melanomas (5 desmoplastic, 4 amelanotic, 6 melanotic), 13 malignant peripheral nerve sheath tumors; 10 schwannomas (1 pigmented), 12 neurofibromas (4 pigmented), and 20 fibrohistiocytic tumors (10 dermatofibrosarcoma protuberans and 10 dermatofibromas). Microwave-based antigen retrieval was performed in 10mM citrate buffer, pH 6.0, for 20 minutes at 121°C. Results.—All melanomas demonstrated positive immunostaining for tyrosinase, HMB-45, and S100 protein. Immunoreactivity for HMB-45 was generally stronger than that for tyrosinase in amelanotic lesions and significantly stronger in 1 of the desmoplastic lesions. The 4 pigmented neurofibromas were focally positive for tyrosinase, but did not stain for HMB-45. The pigmented schwannoma was focally positive for both tyrosinase and HMB-45. The malignant peripheral nerve sheath tumors, dermatofibrosarcoma protuberans, and dermatofibromas were nonreactive for tyrosinase and HMB-45. Conclusions.—Our results support the sensitivity of tyrosinase expression and demonstrate the relative specificity of tyrosinase as a marker for melanocytic lesions, including desmoplastic melanoma, although pigmented peripheral nerve tumors may demonstrate focal positive staining. Immunoreactivity for tyrosinase and HMB-45 may have been enhanced by the microwave-based antigen-retrieval technique used in this study.

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Malignant Melanoma With a Rhabdoid Phenotype: Histologic, Immunohistochemical, and Ultrastructural Study of a Case and Review of the Literature

Archives of Pathology & Laboratory Medicine ◽

10.5858/2004-128-686-mmwarp ◽

2004 ◽

Vol 128 (6) ◽

pp. 686-688

Author(s):

Jared J. Abbott ◽

Robin H. Amirkhan ◽

Mai P. Hoang

Keyword(s):

Malignant Melanoma ◽

Tumor Cells ◽

Rare Variant ◽

Smooth Muscle Actin ◽

S100 Protein ◽

Metastatic Lesion ◽

Primary Tumor Site ◽

Cytoplasmic Inclusions ◽

Rhabdoid Cells ◽

Rhabdoid Tumors

Abstract Malignant melanoma is known to display tremendous histologic diversity. One rare variant is the rhabdoid phenotype, so called because of the appearance of cells resembling rhabdomyoblasts seen in malignant rhabdoid tumors of the kidney. We present the histologic, immunohistochemical, and ultrastructural features of a malignant melanoma composed entirely of rhabdoid cells. A 62-year-old man presented with a 6.5-cm lung mass. Although presumed to be a metastatic lesion, extensive workup failed to reveal a primary tumor site. Histologic sections showed a mass composed entirely of polygonal neoplastic cells with prominent nucleoli and large hyaline cytoplasmic inclusions. The tumor cells were strongly immunoreactive with S100 protein, vimentin, and CD56, and were focally reactive with Mart-1. Tumor cells were negative for Melan-A, tyrosinase, HMB-45, AE1/AE3, cytokeratin (CK) 7, CK8/ 18, CK20, CK903, CAM 5.2, epithelial membrane antigen, smooth muscle actin, desmin, leukocyte common antigen, Bcl-2, CD3, CD20, CD30, CD138, κ and λ light chains, CD68, CD34, factor VIII, synaptophysin, and glial fibrillary acidic protein. Electron microscopy showed cytoplasmic whorls of intermediate filaments containing entrapped rough endoplasmic reticulum, mitochondria, and lipid. Recognition of this rare variant of malignant melanoma is important in the evaluation of tumors with rhabdoid morphology.

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Malignant Perivascular Epithelioid Cell Tumor Involving the Prostate

Archives of Pathology & Laboratory Medicine ◽

10.5858/2003-127-e96-mpecti ◽

2003 ◽

Vol 127 (2) ◽

pp. e96-e98 ◽

Cited By ~ 6

Author(s):

Chin-Chen Pan ◽

An-Hang Yang ◽

Hung Chiang

Keyword(s):

Seminal Vesicle ◽

Tumor Cells ◽

S100 Protein ◽

Epithelioid Cell ◽

Cell Tumor ◽

Epithelioid Cells ◽

Perivascular Epithelioid Cell Tumor ◽

Perivascular Epithelioid Cell ◽

Granular Cytoplasm ◽

Hmb 45

Abstract Perivascular epithelioid cell tumor (PEComa) is a neoplasm chiefly composed of HMB-45–positive epithelioid cells with clear-to-granular cytoplasm and a perivascular distribution. We describe such a tumor involving the prostate and seminal vesicle in a 46-year-old man. The tumor had characteristic histologic features of PEComa. Immunohistochemically, the tumor cells were positive for HMB-45 but negative for epithelial markers, Melan-A, and S100 protein. The tumor behaved in a malignant fashion, and the patient died of the disease 4 years after diagnosis.

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Comparative Immunoreactivity of CO-68 and HMB-45 in Malignant Melanoma, Neural Tumors and Nevi

Pathology - Research and Practice ◽

10.1016/s0344-0338(97)80103-3 ◽

1997 ◽

Vol 193 (7) ◽

pp. 497-502 ◽

Cited By ~ 21

Author(s):

Ifat A. Shah, M.D. ◽

Osama S. Gani, M.D. ◽

Leslie Wheler, R.N.

Keyword(s):

Malignant Melanoma ◽

Hmb 45

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Primary retromolar malignant melanoma in a Latin American patient

Journal of International Medical Research ◽

10.1177/0300060520919245 ◽

2020 ◽

Vol 48 (4) ◽

pp. 030006052091924

Author(s):

Carlos-Martín Ardila ◽

Joan-Michael Arce-Jaramillo ◽

Efraín Álvarez-Martínez

Keyword(s):

Latin America ◽

Malignant Melanoma ◽

Latin American ◽

Surgical Excision ◽

Primary Malignant Melanoma ◽

Ki 67 ◽

Metastatic Lesions ◽

Immunohistochemistry Staining ◽

American Patient ◽

Hmb 45

Primary malignant melanoma of the oral mucosa is rare and has scarcely been reported in patients from Latin America. The most frequently affected sites include the palate and gingiva of the maxilla. In this report, we describe primary malignant melanoma in a Latin American patient. A 3 × 3.5-cm black tumor was observed on the upper left retromolar area. The lesion exhibited a sessile base, irregular edges, soft consistency, and pain on palpation. Immunohistochemistry staining results were positive for S100 and HMB-45; the Ki-67 index was 20%. Contrast tomography of the skull, face, abdomen, and thorax, as well as ultrasound of the liver, did not reveal metastatic lesions. The patient underwent wide surgical excision of the lesion, followed by radiotherapy; he was subsequently rehabilitated with a somato prosthesis.

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