Flow cytometric analysis of intraepithelial lymphocytes from human small intestinal biopsies reveals populations of CD4+CD8+ and CD8αα+ cells

1993 ◽  
Vol 5 (11) ◽  
pp. 907-912 ◽  
Author(s):  
Sara Lynch ◽  
Dermot Kelleher ◽  
Conleth Feighery ◽  
Donald G. Weir ◽  
Cliona OʼFarrelly
Keyword(s):  
Flow Cytometric Analysis ◽  
Intraepithelial Lymphocytes ◽  
Flow Cytometric ◽  
Small Intestinal

scholarly journals GPR18 is required for a normal CD8αα intestinal intraepithelial lymphocyte compartment

2014 ◽  
Vol 211 (12) ◽  
pp. 2351-2359 ◽  
Author(s):  
Xiaoming Wang ◽  
Hayakazu Sumida ◽  
Jason G. Cyster
Keyword(s):  
T Cells ◽  
Lamina Propria ◽  
Flow Cytometric Analysis ◽  
Cell Subset ◽  
Intraepithelial Lymphocytes ◽  
Orphan Receptor ◽  
Flow Cytometric ◽  
Intestinal Intraepithelial Lymphocyte ◽  
Mixed Chimeras ◽  
G Protein Coupled

Intraepithelial lymphocytes (IELs) play an important role in maintaining the physiology of the small intestine. The majority of mouse IELs express CD8αα and are either γδ or αβ T cells. Although the development and homing of CD8αα IELs have been studied in some detail, the factors controlling their homeostasis and positioning are incompletely understood. Here we demonstrate that G protein–coupled receptor 18 (GPR18) is abundantly expressed in CD8αα IELs and that mice lacking this orphan receptor have reduced numbers of γδT IELs. Mixed bone marrow chimera experiments reveal a markedly reduced contribution of GPR18-deficient cells to the CD8αα IEL compartment and a reduction in the CD8αβ T cell subset. These defects could be rescued by transduction with a GPR18-expressing retrovirus. The GPR18-deficient γδT IELs that remained in mixed chimeras had elevated Thy1, and there were less granzyme B+ and Vγ7+ cells, indicating a greater reduction in effector-type cells. Flow cytometric analysis indicated GPR18 deficiency more strongly affected the CD8αα cells in the intraepithelial compared with the adjacent lamina propria compartment. These findings establish a requirement for GPR18 in CD8αα and CD8αβ IELs, and we suggest the receptor has a role in augmenting the accumulation of CD8 T cells in the intraepithelial versus lamina propria compartment.

Flow cytometric analysis of intestinal intraepithelial lymphocytes in the diagnosis of refractory celiac sprue

2008 ◽  
Vol 20 (5) ◽  
pp. 478-487 ◽  
Author(s):  
Laura B. Sanchez-Muñoz ◽  
Almudena Santón ◽  
Ana Cano ◽  
Antonio Lopez ◽  
Julia Almeida ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Intraepithelial Lymphocytes ◽  
Celiac Sprue ◽  
Flow Cytometric ◽  
Intestinal Intraepithelial Lymphocytes

Flow cytometric analysis of colonic and small intestinal mucosal lymphocytes obtained by endoscopic biopsy in the healthy dog

2000 ◽  
Vol 77 (1-2) ◽  
pp. 103-119 ◽  
Author(s):  
Ioana M Sonea ◽  
Albert E Jergens ◽  
Randy E Sacco ◽  
Yoshia Niyo ◽  
Ellen Merten ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Endoscopic Biopsy ◽  
Flow Cytometric ◽  
Small Intestinal
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