Using interactive virtual simulation methods and combining numerical calculation software, the 3D airline emergency evacuation simulation system is established in Virtools platform according to the received data. The system takes full account of the passengers' individual differences during the evacuation process, setting up different member parameters, which influence the passengers evacuation behavior. In order to fulfill the different types of evacuation requirements in various simulation, it can change evacuation path algorithm, cabin layout data and evacuees component parameters, to get the corresponding evacuation simulation results. Meanwhile, the system interface can change visual angle to realize human-machine interaction freely and reflect the evacuation of the evacuation process accurately. Also the reliability of the system simulation result is verified by compared with the results of actual certification trial.