Antiandrogenic actions of medroxyprogesterone acetate on epithelial cells within normal human breast tissues cultured ex vivo

2014 ◽  
Vol 21 (1) ◽  
pp. 79-88 ◽  
Author(s):  
Aleksandra M. Ochnik ◽  
Nicole L. Moore ◽  
Tanja Jankovic-Karasoulos ◽  
Tina Bianco-Miotto ◽  
Natalie K. Ryan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Medroxyprogesterone Acetate ◽  
Ex Vivo ◽  
Human Breast ◽  
Normal Human Breast ◽  
Normal Human ◽  
Breast Tissues

Clonal analysis of expression of epithelial antigens in cultures of normal human breast

1986 ◽  
Vol 80 (1) ◽  
pp. 91-101
Author(s):  
P.A. Edwards ◽  
I.M. Brooks ◽  
H.J. Bunnage ◽  
A.V. Foster ◽  
M.L. Ellison ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Epithelial Cells ◽  
Phase Contrast ◽  
Single Cells ◽  
Full Range ◽  
Human Breast ◽  
Intact Tissue ◽  
Normal Human Breast ◽  
Normal Human ◽  
Luminal Epithelial Cells

Cells from normal human breast epithelium were cloned in monolayer culture and the clones were stained with monoclonal antibodies. Tissue was from reduction mammoplasty operations. Cloning efficiencies were 5–30%. Two types of clone were identified: 10 to 30% were of relatively spread cells whose boundaries were often difficult to see by phase-contrast microscopy but where they were visible they appeared as dark lines. The edges of the clones usually appeared to be under tension. These clones were stained by two monoclonal antibodies, LICR-LON-M8 and M24, that stain luminal epithelial cells in the intact tissue, but not myoepithelial or stromal cells. Within a clone the cells showed a full range of antigenic phenotypes. This was confirmed for clones grown from single cells that had been isolated manually. The second type of clone was more compact with little evidence of tension at the edges, and cell boundaries were clearly visible and bright under phase contrast. These clones were not stained by antibodies M8 or M24. Both types of clone stained with a third monoclonal antibody that is specific for luminal epithelial cells in the intact tissue, LICR-LON-M18, but the distribution of staining was different in the different types of clone. The simplest interpretation of the two types of clone is that luminal epithelial cells give rise to the spread type of clone while the myoepithelial cells give rise to the more abundant and vigorous compact clones. Alternatively, the compact clones may be from luminal epithelial cells that have lost differentiated characteristics.

Depolarization of autofluorescence from malignant and normal human breast tissues

2001 ◽  
Vol 40 (7) ◽  
pp. 1147 ◽  
Author(s):  
Samarendra K. Mohanty ◽  
Nirmalya Ghosh ◽  
Shovan K. Majumder ◽  
Pradeep K. Gupta
Keyword(s):  
Human Breast ◽  
Normal Human Breast ◽  
Normal Human ◽  
Breast Tissues

scholarly journals Cigarette smoke condensate-induced transformation of normal human breast epithelial cells in vitro

Oncogene ◽  
2004 ◽  
Vol 23 (35) ◽  
pp. 5880-5889 ◽  
Author(s):  
Satya Narayan ◽  
Aruna S Jaiswal ◽  
Diana Kang ◽  
Pratima Srivastava ◽  
Gokul M Das ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Cigarette Smoke Condensate ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Normal Human Breast ◽  
Normal Human ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells

Correlation of CD44 expression with proliferative activity of normal human breast epithelial cells in culture

1998 ◽  
Vol 50 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Nancy L. Cooper ◽  
Peter Bardy ◽  
Julinor Bacani ◽  
Urve Kuusk ◽  
Graeme J. Dougherty ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proliferative Activity ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Cd44 Expression ◽  
Cells In Culture ◽  
Normal Human Breast ◽  
Normal Human ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells

scholarly journals Diallyl sulfide inhibits PhIP-induced DNA strand breaks in normal human breast epithelial cells

2007 ◽  
Author(s):  
Chantell Wilson ◽  
Ayoola Aboyade-Cole ◽  
Oneil Newell ◽  
Selina Darling-Reed ◽  
Ebenezer Oriaku ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dna Strand Breaks ◽  
Human Breast ◽  
Diallyl Sulfide ◽  
Breast Epithelial Cells ◽  
Strand Breaks ◽  
Normal Human Breast ◽  
Normal Human ◽  
Dna Strand ◽  
Human Breast Epithelial Cells
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