Novel Method Probe-based Real-Time PCR to Detect 2 Single-Nucleotide Polymorphisms Close to Each Other

2016 ◽  
Vol 24 (9) ◽  
pp. 673-678 ◽  
Author(s):  
Frederico S. V. Malta ◽  
Zilma N. Reis ◽  
Antônio C. V. Cabral
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Real Time Pcr ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Novel Method

Loss of heterozygosity testing using real-time PCR analysis of single nucleotide polymorphisms

2005 ◽  
Vol 132 (3) ◽  
pp. 200-204 ◽  
Author(s):  
Tamara Čačev ◽  
Mladen Jokić ◽  
Radan Spaventi ◽  
Krešimir Pavelić ◽  
Sanja Kapitanović
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Loss Of Heterozygosity ◽  
Real Time Pcr ◽  
Pcr Analysis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms

2004 ◽  
Vol 18 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Kenneth Petersen ◽  
Ulla Vogel ◽  
Eszter Rockenbauer ◽  
Kirsten Vang Nielsen ◽  
Steen Kølvraa ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Real Time Pcr ◽  
Molecular Beacons ◽  
Nucleotide Polymorphisms ◽  
Allelic Discrimination ◽  
Single Nucleotide

scholarly journals Real-Time PCR Assays of Single-Nucleotide Polymorphisms Defining the Major Brucella Clades

2008 ◽  
Vol 46 (7) ◽  
pp. 2474-2474
Author(s):  
J. T. Foster ◽  
R. T. Okinaka ◽  
R. Svensson ◽  
K. Shaw ◽  
B. K. De ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Real Time Pcr ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Pcr Assays

scholarly journals Quantification of donor and recipient hemopoietic cells by real-time PCR of single nucleotide polymorphisms

Leukemia ◽  
2003 ◽  
Vol 17 (3) ◽  
pp. 630-633 ◽  
Author(s):  
F Maas ◽  
N Schaap ◽  
S Kolen ◽  
A Zoetbrood ◽  
I Buño ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Real Time Pcr ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hemopoietic Cells

scholarly journals TaqMan Real-Time PCR Assays for Single-Nucleotide Polymorphisms Which Identify Francisella tularensis and Its Subspecies and Subpopulations

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107964 ◽  
Author(s):  
Dawn N. Birdsell ◽  
Amy J. Vogler ◽  
Jordan Buchhagen ◽  
Ashley Clare ◽  
Emily Kaufman ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Francisella Tularensis ◽  
Real Time Pcr ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Pcr Assays

scholarly journals Il-18 Overproduction Associated with NLRP1 Single Nucleotide Polymorphisms Linked to Risk for Vitiligo

2021 ◽  
Vol 6 (2) ◽  
Keyword(s):  
Gene Expression ◽  
Single Nucleotide Polymorphisms ◽  
Autoimmune Diseases ◽  
Real Time ◽  
Real Time Pcr ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Risk Genotype ◽  
Human Volunteers ◽  
Cytokine Levels

Background The NLRP1gene is central to the NLR inflammasome. Variants to the NLRP1 gene are associated with vitiligo and other autoimmune diseases. We examined the effects of two single nucleotide polymorphisms (SNP) son cytokine levels and NLRP1 gene expression in 50 human volunteers. Methods NLRP1 was genotyped at SNPs rs2670660 and rs12150220, and participants who were homozygous at one or more SNP were analyzed. Plasma IL-18 and IL-1β levels were quantified using ELISA. NLRP1 gene expression was measured using real-time PCR. Results Participants with the risk genotype had significantly higher levels of plasma IL-18 than participants with protective genotype (0.439 ng/µL compared to 0.152 ng/µL, p = 0.024). Genotypes rs2670660 and rs12150220 were strongly linked in this population (p = 2.33 x 10-13). Conclusions Increased production of IL-18, suggests that at least one of the AA variants of rs2670660 or rs12150220 increases NLRP1 activity. rs2670660 and rs12150220 are strongly linked.

The mechanism and regularity of quenching the effect of bases on fluorophores: the base-quenched probe method

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3292-3301 ◽  
Author(s):  
Huihui Mao ◽  
Guanghua Luo ◽  
Yuxia Zhan ◽  
Jun Zhang ◽  
Shuang Yao ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Probe Method ◽  
Melting Curve Analysis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

The base-quenched probe method for detecting single nucleotide polymorphisms (SNPs) relies on real-time PCR and melting-curve analysis, which might require only one pair of primers and one probe.

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