Changes of lymph metabolites in a rat model of sepsis induced by cecal ligation and puncture

2012 ◽  
Vol 73 (6) ◽  
pp. 1545-1552 ◽  
Author(s):  
Yan Li ◽  
Ming Hou ◽  
Jian-guang Wang ◽  
Tao Wang ◽  
Jian Wan ◽  
...  
Keyword(s):  
Rat Model ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture

Abstract 320: End-Tidal Carbon Dioxide Can Guide Fluid Resuscitation in a Cecal Ligation and Puncture Induced Rat Model of Sepsis

Circulation ◽  
2018 ◽  
Vol 138 (Suppl_2) ◽  
Author(s):  
Jing Xu ◽  
Guanghui Zheng ◽  
Liangliang Wu ◽  
Xiangshao Fang ◽  
Yue Wang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Survival Time ◽  
Fluid Resuscitation ◽  
Rat Model ◽  
Cecal Ligation ◽  
Control Group ◽  
Cecal Ligation And Puncture ◽  
End Tidal Carbon Dioxide ◽  
Lactate Levels ◽  
End Tidal

Introduction: Abnormal levels of end-tidal carbon dioxide (ETCO 2 ) may reflect a derangement in perfusion, metabolism, or gas exchange. It is unclear if ETCO 2 can be used for fluid resuscitation (FR) compared with traditional mean arterial pressure (MAP) as an outcome predictor in sepsis. Hypothesis: Use of ETCO2 is better than MAP in guiding fluid resuscitation to improve lactate levels and microcirculatory blood flow in sepsis. Methods: Thirty-five male Sprague-Dawley rats weighing 350-400g were randomized to: 1) SHAM, n=5; 2) cecal ligation and puncture (CLP) Control group (with CLP, without FR, n=10); 3) ETCO 2 group (with CLP, FR began when ETCO 2 ≤25 mmHg, n=10) and 4) MAP group (with CLP, FR began when MAP≤100 mmHg, n=10). Lactate level, cardiac output (CO), perfused small vessel density (PSVD) and sublingual microvascular flow index (MFI) was assessed at baseline, 2 h, 4 h, 8 h, 10 h and 12 h post-CLP. Survival duration was recorded. Results: After FR,CO in the ETCO 2 group increased compared with the MAP group 12h after CLP while lactate levels decreased compared with the Control and MAP groups (p<0.05) (Figure-1). Both sublingual PSVD and MFI decreased after CLP in the control group and MAP group but significantly improved in the ETCO 2 group 8h post-CLP. The average survival time in the ETCO 2 group was significantly greater than MAP group (Figure-2). Conclusions: ETCO 2 guided FR was associated with improved CO, lactate, microcirculatory flow, and survival time compared to MAP guided FR in a CLP-induced rat model of sepsis.

CD123 thioaptamer protects against sepsis via the blockade between IL-3/CD123 in a cecal ligation and puncture rat model

2020 ◽  
Vol 40 (1) ◽  
pp. 16-31
Author(s):  
Jiangang Zhao ◽  
Meng Wang ◽  
Ying Yang ◽  
Guoxia Wang ◽  
Fengyu Che ◽  
...  
Keyword(s):  
Rat Model ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture

scholarly journals Autonomic control in a rat model of sepsis induced by cecal ligation and puncture

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Glaucia Raquel Luciano Veiga ◽  
Lila Missay Oyama ◽  
Rubens Fazan ◽  
Roberto Pontes ◽  
Ruy Campos ◽  
...  
Keyword(s):  
Rat Model ◽  
Cecal Ligation ◽  
Autonomic Control ◽  
Cecal Ligation And Puncture

Abstract 330: LDK378 Attenuates Macro and Microcirculation in a Sepsis Rat Model Induced by Cecal Ligation and Puncture

Circulation ◽  
2018 ◽  
Vol 138 (Suppl_2) ◽  
Author(s):  
Weiwei Ge ◽  
Zhengfei Yang ◽  
Qiaohua Hu ◽  
Xuefen Liu ◽  
Qin Ling ◽  
...  
Keyword(s):  
Rat Model ◽  
Inflammatory Mediator ◽  
Myocardial Dysfunction ◽  
Cecal Ligation ◽  
Male Rats ◽  
Flow Index ◽  
Cecal Ligation And Puncture ◽  
Sublingual Microcirculation ◽  
Sepsis Model ◽  
Severe Infections

Background: Sepsis is a systemic inflammatory response syndrome caused by severe infections. Myocardial dysfunction has been associated with increased mortality in septic patients. Previous studies have demonstrated that LDK378, a second-generation ALK inhibitor, is a potential anti-inflammatory mediator. We investigated the effects of LDK378 on macro and microcirculation in a rat model of cecal ligation and puncture (CLP) induced sepsis. Hypothesis: LDK378 attenuates the deleterious effects of sepsis on macro- and micro- circulation in CLP induced septic rats. Method: Fifteen male rats weighing between 450-550g were randomized into three groups: Sham, CLP, and CLP+LDK378. Our rat sepsis model was induced by 50% cecum ligation and two punctures with a 20-gauge needle. LDK378 (20mg/kg) was administered intraperitoneal (IP) to animals at 0h, 24h and 48 hours after CLP operation in the CLP+LDK378 group. Cardiac output (CO), mean arterial pressure (MAP); sublingual microcirculation including perfused small vessel density (PVD) and microcirculatory flow index (MFI) were measured at 6h, 30h and 54h after CLP. Results: Significant reduction in CO, MAP, MFI and PVD were observed in both CLP and CLP+LDK378 groups at 6h, 30h and 54h. However, the levels of CO, MAP, MFI and PVD reduction were significantly better in the CLP+LDK378 group when compared with the CLP group at 30h and 54h after CLP. Conclusion: LDK378 attenuates macro and microcirculation parameters in our rat model of sepsis.

Abstract P551: Platelet Activation is Associated with Pulmonary Edema and Renal Injury in a Rat Model of Polymicrobial Sepsis

Hypertension ◽  
2017 ◽  
Vol 70 (suppl_1) ◽  
Author(s):  
Jeanne Ishimwe ◽  
Maggie L McCalmon ◽  
Corbin A Shields ◽  
Ashley Gnam ◽  
Jan M Williams ◽  
...  
Keyword(s):  
Renal Function ◽  
Pulmonary Edema ◽  
Organ Dysfunction ◽  
Platelet Activation ◽  
Rat Model ◽  
Renal Injury ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
Activated Platelets ◽  
Underlying Mechanisms

Sepsis, life-threatening organ dysfunction due to a dysregulated host response to infection, is positively correlated with platelet activation. Furthermore, clinical studies have also shown that platelet activation is associated with sepsis severity, suggesting a role for platelets in sepsis pathophysiology. Despite this correlation, the underlying mechanisms by which activated platelets contribute to sepsis are under investigated. In preliminary studies, we set out to determine if platelet activation is associated with multi-organ dysfunction and injury in a rat model of chronic polymicrobial abdominal sepsis. Sepsis was induced via cecal ligation and puncture (CLP) followed by cecum removal 24 hours post-CLP. At 72 hours post-CLP, blood, urine, and tissues were collected for analysis. Platelet activation was measured via flow cytometry. Lung wet/dry ratio and plasma creatinine were measured to assess lung edema and renal injury, respectively. Platelet activation doubled in CLP rats versus Sham rats. Activated platelets increased from 3.8±1.7% of the gated population in Sham animals (n=5) to 9.2±1.9% of the gated population in CLP animals (n=5; p=0.07). Lung wet/dry ratio significantly increased from 3.9±0.2 in Sham (n=8) to 6.7±1 in CLP rats (n=8; p<0.05). Furthermore, plasma creatinine increased by 33% from 0.55±0.3 mg/dL in Sham animals (n=6) to 0.73±0.06 mg/dL in CLP rats (n=8; p<0.05), indicating a decrease in renal function. These data demonstrate, for the first time, an increase in platelet activation in response to CLP, and identifies an association of activated platelets with pulmonary edema and reduced renal function in the cecal ligation and puncture rat model of abdominal polymicrobial sepsis. Future studies will investigate the underlying mechanisms by which activated platelets contribute to multi-organ dysfunction and injury in sepsis.

Effects of End-Tidal Carbon Dioxide-Guided Fluid Resuscitation on Outcomes in a Cecal Ligation and Puncture Induced Rat Model of Sepsis

Shock ◽  
2019 ◽  
Vol 51 (4) ◽  
pp. 519-525 ◽  
Author(s):  
Jing Xu ◽  
Guanghui Zheng ◽  
Liangliang Wu ◽  
Xiangshao Fang ◽  
Yue Wang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Fluid Resuscitation ◽  
Rat Model ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
End Tidal Carbon Dioxide ◽  
End Tidal

The inhibitor of interleukin-3 receptor protects against sepsis in a rat model of cecal ligation and puncture

2019 ◽  
Vol 109 ◽  
pp. 71-80 ◽  
Author(s):  
Juntao Hu ◽  
Zhanhong Tang ◽  
Jing Xu ◽  
Weiwei Ge ◽  
Qiaohua Hu ◽  
...  
Keyword(s):  
Rat Model ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
Interleukin 3

scholarly journals Analysis of blood culture in a rat model of cecal ligation and puncture induced sepsis

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Prabakar Vaittinada Ayar ◽  
Hervé Jacquier ◽  
Benjamin Deniau ◽  
Feriel Azibani ◽  
Alexandre Mebazaa ◽  
...  
Keyword(s):  
Blood Culture ◽  
Rat Model ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture
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