scholarly journals Concordance Among Pathologists in the Second Cardiac Allograft Rejection Gene Expression Observational Study (CARGO II)

2012 ◽  
Vol 94 (11) ◽  
pp. 1172-1177 ◽  
Author(s):  
Maria G. Crespo-Leiro ◽  
Andreas Zuckermann ◽  
Christoph Bara ◽  
Paul Mohacsi ◽  
Uwe Schulz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Observational Study ◽  
Allograft Rejection ◽  
Cardiac Allograft ◽  
Cardiac Allograft Rejection

Abstract 19055: MicroRNA Gene Expression of Heart Transplant Endomyocardial Biopsy

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
Eleanor Chang ◽  
Gregory Fishbein ◽  
Maral Bakir ◽  
Galyna Bondar ◽  
Nicholas Jackson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endomyocardial Biopsy ◽  
Allograft Rejection ◽  
Empirical Bayes ◽  
Expression Profiles ◽  
Target Prediction ◽  
Cardiac Allograft ◽  
Protein Kinase Activity ◽  
Cardiac Allograft Rejection ◽  
Clinical Phenotypes

Introduction Endomyocardial biopsy is the standard surveillance method to detect cardiac allograft rejection. While microRNAs (miRNA) play a major role in regulating mRNA, their nature and role in the biology is not well understood. We hypothesized that specific mRNA-miRNA networks can be identified underlying the clinical phenotypes of different forms of cardiac allograft rejection. Method Twenty one tissue samples from 14 post-HTx patients were subjected to genome wide miRNA sequencing. A non-parametric empirical Bayes framework removed batch effect and filtered genes with low variability. Weighted Gene Correlation Network Analysis (WGCNA) clustered genes into related eigengene modules based on their gene expression. Identified miRNAs were subjected to target prediction and compared with mRNA expression profiles previously identified on the same biopsies. Gene Ontology (GO) was used for biological interpretation of selected genes. Results 1270 miRNAs were used to construct 9 eigengene modules. Module-Trait relationship were then investigated as shown in Figure. The top ten miRNA probe sets filtered by the highest intra-module correlation and statistical significance were hsa-miR-141-3p, hsa-miR-150-5p, hsa-miR-605, hsa-miR-582-5p, hsa-miR-3150b-3p, hsa-miR-508-3p, hsa-miR-652-5p, hsa-miR-26a-1-3p, hsa-miR-3667-3p and hsa-miR-3911. Target prediction analysis resulted in 724 gene targets. GO analysis revealed 184 categories enriched by these genes including regulation of protein kinase activity, cardiac muscle cell differentiation and epithelial cell migration among others. Compared to mRNA previously identified in the same heart biopsies showed 685 overlapping gene targets. Conclusion WGCNA identified miRNA modules correlated with different clinical phenotypes of rejection. MRNA-miRNA pairs were identified to help understand the biology of rejection and as interesting candidates for diagnostic or therapeutic applications.

CD27 but not CD70 and 4-1BB intragraft gene expression is a risk factor for acute cardiac allograft rejection in humans

2002 ◽  
Vol 34 (2) ◽  
pp. 474-475 ◽  
Author(s):  
N Shulzhenko ◽  
A Morgun ◽  
A.P Chinellato ◽  
G.F Rampim ◽  
R.V.Z Diniz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Risk Factor ◽  
Allograft Rejection ◽  
Cardiac Allograft ◽  
Cardiac Allograft Rejection

scholarly journals Detection of Cardiac Allograft Rejection and Response to Immunosuppressive Therapy With Peripheral Blood Gene Expression

Circulation ◽  
2004 ◽  
Vol 110 (25) ◽  
pp. 3815-3821 ◽  
Author(s):  
Phillip A. Horwitz ◽  
Emily J. Tsai ◽  
Mary E. Putt ◽  
Joan M. Gilmore ◽  
John J. Lepore ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immunosuppressive Therapy ◽  
Peripheral Blood ◽  
Allograft Rejection ◽  
Cardiac Allograft ◽  
Blood Gene Expression ◽  
Cardiac Allograft Rejection ◽  
Peripheral Blood Gene Expression

The physiological and pathophysiological modulation of the endocrine function of the heart

2001 ◽  
Vol 79 (8) ◽  
pp. 705-714 ◽  
Author(s):  
Adolfo J de Bold ◽  
Kenneth K -Y Ma ◽  
Ying Zhang ◽  
Mercedes L Kuroski de Bold ◽  
Michael Bensimon ◽  
...  
Keyword(s):  
Gene Expression ◽  
G Protein ◽  
Allograft Rejection ◽  
Ventricular Hypertrophy ◽  
Cardiac Allograft ◽  
Cardiac Allograft Rejection ◽  
Atrial Muscle ◽  
Np Gene ◽  
G Protein Coupled

Under physiological conditions, the endocrine heart contributes to the maintenance of cardiovascular homeostasis through the polypeptide hormones ANF and BNP, which are members of the natriuretic peptide (NP) family. Given that NPs are of interest from the basic and clinical points of view, the genetic expression and secretion of ANF and BNP as well as the nature of the interaction of these hormones with their receptors has been the subject of extensive studies since the discovery of ANF in 1980. Following hemodynamic overload, increased secretion of NPs by the heart can be seen. This change may occur without an increase in gene expression as observed for atrial NPs following acute volume expansion, or it can occur with an increase in both ANF and BNP gene expression in atria only as seen in mineralocorticoid escape during which it is obvious that a critical decrease in hormone stores must be reached before transcriptional activation occurs. Chronic hemodynamic pressure or volume overload results in increased expression of NPs in atria and ventricles. Under these circumstances, the increased production of BNP by hypertrophic ventricles changes the normal plasma concentration ratio of ANF to BNP, a fact that has clinical diagnostic and prognostic implications. There are exceptions to this rule: chronic, severe L-NAME hypertension, which may occur without left ventricular hypertrophy, does not cause this effect and increased ventricular NP gene expression can occur in mineralocorticoid hypertension before detectable ventricular hypertrophy. Atrial and ventricular NP gene expression appears to be under different transcriptional control because pharmacological treatments such as chronic ACE inhibition or ETA receptor blockade can reverse the increased ventricular NP expression but has no detectable effect on atrial NP gene expression. This is not unlike the myosin heavy chain switch that is observed in certain pathologies, and can be pharmacologically reversed in a manner similar to NPs in the ventricles but it does not occur in atrial muscle. These observations made in vivo or using isolated adult atria often differ strikingly from results obtained using the mixed phenotype afforded by cardiocytes in culture, indicating that the kinds of questions addressed by each approach must be judiciously chosen. G-protein coupled receptor-mediated actions of neurohumors such as endothelin and phenylephrine are normally used to stimulate NP gene expression and release in different in vitro models. The main physiological stimulus for increased ANF release, atrial muscle stretch, also appears to rely on G-protein-coupled mechanisms. Alternative agonists and receptor types at play are suggested by the finding that circulating levels of BNP are selectively increased before and during overt cardiac allograft rejection episodes in human patients. The data suggest that enhanced BNP plasma levels could form a basis for a noninvasive test for cardiac allograft rejection. However, the molecular mechanism by which expression of NPs are regulated in the transplanted heart is not well understood. Conditioned medium from mixed lymphocyte reaction cultures, considered an in vitro model of transplantation immunity, induces specific upregulation of BNP as do individual pro-inflammatory cytokines. Findings such as these suggest that the study of NPs will continue to produce a wealth of information relevant to basic and clinical scientists.Key words: atrial natriuretic factor, hypertension, hypertrophy, heart failure, cytokines.

DIFFERENTIAL NF-??B AND I??B GENE EXPRESSION DURING DEVELOPMENT OF CARDIAC ALLOGRAFT REJECTION VERSUS CD154 MONOCLONAL ANTIBODY-INDUCED TOLERANCE1

2001 ◽  
Vol 71 (7) ◽  
pp. 835-840 ◽  
Author(s):  
Vilmos Csizmadia ◽  
Wei Gao ◽  
Saege A. Hancock ◽  
James B. Rottman ◽  
Zihao Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Monoclonal Antibody ◽  
Allograft Rejection ◽  
Cardiac Allograft ◽  
Cardiac Allograft Rejection

171: Does induction therapy influence the utility of gene expression testing for cardiac allograft rejection?

2007 ◽  
Vol 26 (2) ◽  
pp. S121
Author(s):  
M. Mehra ◽  
M.C. Deng ◽  
R. Starling ◽  
S. Murali ◽  
D. Pauly ◽  
...  
Keyword(s):  
Gene Expression ◽  
Induction Therapy ◽  
Allograft Rejection ◽  
Cardiac Allograft ◽  
Cardiac Allograft Rejection
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