Improving the glial differentiation of human Schwann-like adipose-derived stem cells with graphene oxide substrates

There is urgent need to improve the clinical outcome of peripheral nerve injury. Many efforts are directed towards the fabrication of bioengineered conduits, which could deliver stem cells to the site of injury to promote and guide peripheral nerve regeneration. The aim of this study is to assess whether graphene and related nanomaterials can be useful in the fabrication of such conduits. A comparison is made between graphene oxide (GO) and reduced GO substrates. Our results show that the graphene substrates are highly biocompatible, and the reduced GO substrates are more effective in increasing the gene expression of the biomolecules involved in the regeneration process compared to the other substrates studied.

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Current progress in use of adipose derived stem cells in peripheral nerve regeneration

World Journal of Stem Cells ◽

10.4252/wjsc.v7.i1.51 ◽

2015 ◽

Vol 7 (1) ◽

pp. 51 ◽

Cited By ~ 37

Author(s):

Shomari DL Zack-Williams

Keyword(s):

Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Adipose Derived Stem Cells

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The influence of reduced graphene oxide on stem cells: a perspective in peripheral nerve regeneration

Regenerative Biomaterials ◽

10.1093/rb/rbab032 ◽

2021 ◽

Vol 8 (4) ◽

Author(s):

Xiangyun Yao ◽

Zhiwen Yan ◽

Xu Wang ◽

Huiquan Jiang ◽

Yun Qian ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Graphene Oxide ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Reduced Graphene Oxide ◽

Peripheral Nerve Regeneration ◽

Embryonic Stem ◽

Composite Scaffolds ◽

Reduced Graphene

Abstract Graphene and its derivatives are fascinating materials for their extraordinary electrochemical and mechanical properties. In recent decades, many researchers explored their applications in tissue engineering and regenerative medicine. Reduced graphene oxide (rGO) possesses remarkable structural and functional resemblance to graphene, although some residual oxygen-containing groups and defects exist in the structure. Such structure holds great potential since the remnant-oxygenated groups can further be functionalized or modified. Moreover, oxygen-containing groups can improve the dispersion of rGO in organic or aqueous media. Therefore, it is preferable to utilize rGO in the production of composite materials. The rGO composite scaffolds provide favorable extracellular microenvironment and affect the cellular behavior of cultured cells in the peripheral nerve regeneration. On the one hand, rGO impacts on Schwann cells and neurons which are major components of peripheral nerves. On the other hand, rGO-incorporated composite scaffolds promote the neurogenic differentiation of several stem cells, including embryonic stem cells, mesenchymal stem cells, adipose-derived stem cells and neural stem cells. This review will briefly introduce the production and major properties of rGO, and its potential in modulating the cellular behaviors of specific stem cells. Finally, we present its emerging roles in the production of composite scaffolds for nerve tissue engineering.

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