scholarly journals Characterization of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, and Bacillus amyloliquefaciens by Pyrolysis Gas-Liquid Chromatography, Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization, Biochemical Tests, and API Systems

1980 ◽  
Vol 30 (2) ◽  
pp. 448-459 ◽  
Author(s):  
A. G. O'DONNELL ◽  
J. R. NORRIS ◽  
R. C. W. BERKELEY ◽  
D. CLAUS ◽  
T. KANEKO ◽  
...  
1972 ◽  
Vol 44 (6) ◽  
pp. 1058-1061 ◽  
Author(s):  
E. Reiner ◽  
Judy J. Hicks ◽  
Mary M. Ball ◽  
William J. Martin

2017 ◽  
pp. 73
Author(s):  
Jerzy Rzedowski ◽  
Evangelina Ortiz

Using gas-liquid chromatography and analyzing terpenoid contents the clarification of some taxonomic problems in Bursera is attempted. Initial experiences indicated a considerable intraspecific chemical variability and it was found that sampling of various individuals per population is necessary in order to achieve an adequate characterization of most of the species. A concrete case is studied of a population from the region of Cañón del Zopilote (state of Guerrero, Mexico), distinguished by a conspicuous pubescence and apparently reproductively isolated from remaining individuals of B. schlechtendalii Engl., which are perfectly glabrous. The terpenoid spectrum of this population is shown to be significantly different and such fact allows to determine its status of independent species, described as such in this paper.


1982 ◽  
Vol 45 (3) ◽  
pp. 229-233 ◽  
Author(s):  
NORMAN J. STERN

Pyrolysis gas-liquid chromatography (PGLC) and stepwise discriminant analysis (SDA) were ineffective when used to differentiate selected genera, species and strains of foodborne microorganisms. Each of 18 individual bacterial strains analyzed was grown, harvested and subjected to PGLC analysis. The resulting pyrolysis products were separated on a high resolution capillary column and the elution patterns (pyrograms) were subjected to stepwise discriminant analysis of 26 (a–z) characteristic peaks. Classification with the combination of PGLC and SDA was 87% accurate for gram-negative strains of bacteria and 94% accurate for gram-positive strains of bacteria. PGLC-stepwise discriminant· analysis correctly discriminated 80% of the bacterial strains according to the known gram-stain reactions. Only 63% were correctly classified to the genus level when all samples were compared. These findings point out the weak points for this method of bacterial analysis.


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