scholarly journals The AP-1 binding site in the feline immunodeficiency virus long terminal repeat is not required for virus replication in feline T lymphocytes

1993 ◽  
Vol 74 (8) ◽  
pp. 1573-1580 ◽  
Author(s):  
T. Miyazawa ◽  
M. Kohmoto ◽  
Y. Kawaguchi ◽  
K. Tomonaga ◽  
T. Toyosaki ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Long Terminal Repeat ◽  
Binding Site ◽  
Virus Replication ◽  
Feline Immunodeficiency Virus ◽  
Terminal Repeat ◽  
Immunodeficiency Virus

scholarly journals Construction and In Vitro Characterization of Attenuated Feline Immunodeficiency Virus Long Terminal Repeat Mutant Viruses

2001 ◽  
Vol 75 (2) ◽  
pp. 1054-1060 ◽  
Author(s):  
Luisa Bigornia ◽  
Kristen M. Lockridge ◽  
Ellen E. Sparger
Keyword(s):  
Long Terminal Repeat ◽  
Virus Replication ◽  
Feline Immunodeficiency Virus ◽  
Terminal Repeat ◽  
Immunodeficiency Virus ◽  
Activation Pathways ◽  
Virus Expression ◽  
Transcriptional Elements

ABSTRACT AP-1- and ATF-binding sites are cis-acting transcriptional elements within the U3 domain of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) that serve as targets for cellular activation pathways and may regulate virus replication. We report that FIV LTR mutant proviruses encoding U3 deletions of the ATF-binding sequence exhibited restricted virus expression and replication in both feline lymphocytes and macrophages. In contrast, deletion of the AP-1 site had negligible effects on virus expression and replication. FIV LTR mutant proviruses encoding deletions of both the AP-1 and ATF sites or a 72-bp deletion encompassing the AP-1 site, duplicated C/EBP sites, and ATF sites were severely restricted for virus expression. These results demonstrate that deletion of either the ATF-binding site or multiplecis-acting transcriptional elements attenuates FIV. These attenuated FIV mutants provide opportunities to characterize the role of cis-acting elements in virus replication in vivo and to test LTR mutants as attenuated virus vaccines.

Regulation of gene expression directed by the long terminal repeat of the feline immunodeficiency virus

Virology ◽  
1992 ◽  
Vol 187 (1) ◽  
pp. 165-177 ◽  
Author(s):  
Ellen E. Sparger∗ ◽  
Barbara L. Shacklett ◽  
Lisa Renshaw-Gegg ◽  
Peter A. Barry ◽  
Niels C. Pedersen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Long Terminal Repeat ◽  
Feline Immunodeficiency Virus ◽  
Regulation Of Gene Expression ◽  
Terminal Repeat ◽  
Immunodeficiency Virus

Activation of feline immunodeficiency virus long terminal repeat by feline herpesvirus type 1

Virology ◽  
1991 ◽  
Vol 184 (1) ◽  
pp. 449-454 ◽  
Author(s):  
Yasushi Kawaguchi ◽  
Takayuki Miyazawa ◽  
Taisuke Horimoto ◽  
Shin-Ichi Itagaki ◽  
Masashi Fukasawa ◽  
...  
Keyword(s):  
Long Terminal Repeat ◽  
Feline Immunodeficiency Virus ◽  
Terminal Repeat ◽  
Herpesvirus Type ◽  
Immunodeficiency Virus ◽  
Feline Herpesvirus ◽  
Feline Herpesvirus Type 1
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