Biochemical and genetic characterization of Rbf1p, a putative transcription factor of Candida albicans

Microbiology ◽  
1997 ◽  
Vol 143 (2) ◽  
pp. 429-435 ◽  
Author(s):  
N. Ishii ◽  
M. Yamamoto ◽  
F. Yoshihara ◽  
M. Arisawa ◽  
Y. Aoki
Keyword(s):  
Transcription Factor ◽  
Candida Albicans ◽  
Genetic Characterization ◽  
Putative Transcription Factor

Biochemical and genetic characterization of the porcine Prophet of Pit-1 pituitary transcription factor

2000 ◽  
Vol 168 (1-2) ◽  
pp. 77-87 ◽  
Author(s):  
Kyle W. Sloop ◽  
Amy McCutchan Schiller ◽  
Timothy P.L. Smith ◽  
John R. Blanton ◽  
Gary A. Rohrer ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Genetic Characterization

scholarly journals Identification and Characterization of Aspergillus nidulans Mutants Impaired in Asexual Development under Phosphate Stress

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1520 ◽  
Author(s):  
Ainara Otamendi ◽  
Eduardo A. Espeso ◽  
Oier Etxebeste
Keyword(s):  
Transcription Factor ◽  
Aspergillus Nidulans ◽  
Genomic Library ◽  
Phenotypic Trait ◽  
Mutant Form ◽  
Multicopy Suppressor ◽  
Putative Transcription Factor ◽  
Recessive Mutant ◽  
Identification And Characterization

The transcription factor BrlA plays a central role in the production of asexual spores (conidia) in the fungus Aspergillus nidulans. BrlA levels are controlled by signal transducers known collectively as UDAs. Furthermore, it governs the expression of CDP regulators, which control most of the morphological transitions leading to the production of conidia. In response to the emergence of fungal cells in the air, the main stimulus triggering conidiation, UDA mutants such as the flbB deletant fail to induce brlA expression. Nevertheless, ΔflbB colonies conidiate profusely when they are cultured on a medium containing high H2PO4− concentrations, suggesting that the need for FlbB activity is bypassed. We used this phenotypic trait and an UV-mutagenesis procedure to isolate ΔflbB mutants unable to conidiate under these stress conditions. Transformation of mutant FLIP166 with a wild-type genomic library led to the identification of the putative transcription factor SocA as a multicopy suppressor of the FLIP (Fluffy, aconidial, In Phosphate) phenotype. Deregulation of socA altered both growth and developmental patterns. Sequencing of the FLIP166 genome enabled the identification and characterization of PmtCP282L as the recessive mutant form responsible for the FLIP phenotype. Overall, results validate this strategy for identifying genes/mutations related to the control of conidiation.

Mapping of functional domains and characterization of the transcription factor Cph1 that mediate morphogenesis in Candida albicans

2015 ◽  
Vol 83 ◽  
pp. 45-57 ◽  
Author(s):  
Protiti Maiti ◽  
Priyanka Ghorai ◽  
Sumit Ghosh ◽  
Mohan Kamthan ◽  
Rakesh Kumar Tyagi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Candida Albicans ◽  
Functional Domains

scholarly journals Chromosome 5 of human pathogenCandida albicanscarries multiple genes for negative control of caspofungin and anidulafungin susceptibility

2016 ◽  
pp. AAC.01888-16 ◽  
Author(s):  
Sumanun Suwunnakorn ◽  
Hironao Wakabayashi ◽  
Elena Rustchenko
Keyword(s):  
Transcription Factor ◽  
Candida Albicans ◽  
Fungal Pathogen ◽  
Negative Control ◽  
Negative Regulation ◽  
Chromosome 5 ◽  
Multiple Genes ◽  
Putative Transcription Factor ◽  
Reversible Loss ◽  
Major Drug

Candida albicansis an important fungal pathogen with a diploid genome that can adapt to caspofungin, a major drug from the echinocandin class, by a reversible loss of one copy of chromosome 5 (Ch5). Here we explore a hypothesis that more than one gene for negative regulation of echinocandin tolerance is carried on Ch5. We constructedC. albicansstrains that each lacked one of the following Ch5 genes:CHT2for chitinase;PGA4for glucanosyltransferase, andCSU51, a putative transcription factor. We demonstrate that independent deletion of each of these genes increased caspofungin and anidulafungin, another echinocandin, tolerance. Our data indicate that Ch5 carries multiple genes for negative control of echinocandin tolerance, although the final number has yet to be established.

scholarly journals Identification and Functional Characterization of Rca1, a Transcription Factor Involved in both Antifungal Susceptibility and Host Response in Candida albicans

2012 ◽  
Vol 11 (7) ◽  
pp. 916-931 ◽  
Author(s):  
Patrick Vandeputte ◽  
Sylvain Pradervand ◽  
Françoise Ischer ◽  
Alix T. Coste ◽  
Sélène Ferrari ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Candida Albicans ◽  
Signaling Pathway ◽  
Large Scale ◽  
Antifungal Susceptibility ◽  
Functional Characterization ◽  
Therapeutic Strategies ◽  
Content Type ◽  
High Level

ABSTRACTThe identification of novel transcription factors associated with antifungal response may allow the discovery of fungus-specific targets for new therapeutic strategies. A collection of 241Candida albicanstranscriptional regulator mutants was screened for altered susceptibility to fluconazole, caspofungin, amphotericin B, and 5-fluorocytosine. Thirteen of these mutants not yet identified in terms of their role in antifungal response were further investigated, and the function of one of them, a mutant of orf19.6102 (RCA1), was characterized by transcriptome analysis. Strand-specific RNA sequencing and phenotypic tests assigned Rca1 as the regulator of hyphal formation through the cyclic AMP/protein kinase A (cAMP/PKA) signaling pathway and the transcription factor Efg1, but also probably through its interaction with a transcriptional repressor, most likely Tup1. The mechanisms responsible for the high level of resistance to caspofungin and fluconazole observed resulting fromRCA1deletion were investigated. From our observations, we propose that caspofungin resistance was the consequence of the deregulation of cell wall gene expression and that fluconazole resistance was linked to the modulation of the cAMP/PKA signaling pathway activity. In conclusion, our large-scale screening of aC. albicanstranscription factor mutant collection allowed the identification of new effectors of the response to antifungals. The functional characterization of Rca1 assigned this transcription factor and its downstream targets as promising candidates for the development of new therapeutic strategies, as Rca1 influences host sensing, hyphal development, and antifungal response.

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