Actinophytocola xinjiangensis sp. nov., isolated from virgin forest soil

2011 ◽  
Vol 61 (12) ◽  
pp. 2928-2932 ◽  
Author(s):  
Xiaoxuan Guo ◽  
Danheng Qiu ◽  
Jisheng Ruan ◽  
Ying Huang
Keyword(s):  
Forest Soil ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
West China ◽  
North West ◽  
Virgin Forest ◽  
Phyletic Line ◽  
Isoprenoid Quinones

An aerobic, non-motile actinobacterium, strain QAIII60T, was isolated from virgin forest soil of Kanas Nature Reserve, Xinjiang, north-west China. The isolate produced a very scant aerial mycelium that fragmented into cylindrical spores and a non-fragmented substrate mycelium with occasional septa. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, ribose and rhamnose (trace). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0, iso-C16 : 1 H and C17 : 1ω6c. The isoprenoid quinones consisted of MK-9(H4) and MK-10(H2). The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QAIII60T formed a distinct phyletic line that was most closely, albeit loosely, associated with the genus Actinophytocola. A number of physiological characteristics differentiated the isolate from members of the genus Actinophytocola. On the basis of these data, we propose that strain QAIII60T ( = CGMCC 4.4663T  = NBRC 106673T) be assigned as the type strain of a novel species, Actinophytocola xinjiangensis sp. nov.

Actinoallomurus acaciae sp. nov., an endophytic actinomycete isolated from Acacia auriculiformis A. Cunn. ex Benth.

2010 ◽  
Vol 60 (3) ◽  
pp. 554-559 ◽  
Author(s):  
Arinthip Thamchaipenet ◽  
Chantra Indananda ◽  
Chakrit Bunyoo ◽  
Kannika Duangmal ◽  
Atsuko Matsumoto ◽  
...  
Keyword(s):  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Acacia Auriculiformis ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Endophytic Actinomycete ◽  
Phyletic Line ◽  
Actinomycete Strain ◽  
Short Spiral

A novel endophytic actinomycete, strain GMKU 931T, was isolated from the root of a wattle tree, Acacia auriculiformis A. Cunn. ex Benth., collected at Kasetsart University, Bangkok, Thailand. Strain GMKU 931T produced short spiral chains of smooth-surfaced spores on the aerial mycelium. Lysine and meso-diaminopimelic acid were present in the cell-wall peptidoglycan. Whole-cell hydrolysates contained galactose, madurose and mannose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major fatty acids were iso-C16 : 0 and iso-C16 : 1. The major phospholipids were phosphatidylinositol and phosphatidylglycerol. A phylogenetic analysis based on 16S rRNA gene sequences suggested that strain GMKU 931T forms a distinct phyletic line within the recently proposed genus Actinoallomurus. The significant differences in phenotypic and genotypic data indicate that strain GMKU 931T represents a novel species of the genus Actinoallomurus, for which the name Actinoallomurus acaciae sp. nov. is proposed. The type strain is GMKU 931T (=BCC 28622T =NBRC 104354T =NRRL B-24610T).

scholarly journals Amycolatopsis salitolerans sp. nov., a filamentous actinomycete isolated from a hypersaline habitat

2012 ◽  
Vol 62 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Tong-Wei Guan ◽  
Zhan-Feng Xia ◽  
Shu-Kun Tang ◽  
Nan Wu ◽  
Zheng-Jun Chen ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
West China ◽  
North West ◽  
Actinomycete Strain

A novel actinomycete strain, designated TRM F103T, was isolated from a hypersaline habitat of the Tarim basin in Xinjiang province, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Amycolatopsis and was most closely related to Amycolatopsis halophila YIM 93223T (99.3 % 16S rRNA gene sequence similarity). However, DNA–DNA relatedness between these two strains, based on triplicate experiments, was only 31.6 %. The isolate contained meso-diaminopimelic acid and ribose, glucose and galactose as the major whole-cell sugars. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C16 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and glucosamine-containing phospholipids. The G+C content of the genomic DNA was 66.4 mol%. The phenotypic data clearly distinguished the isolate from its closest relatives. The combined phylogenetic, chemotaxonomic and phenotypic data indicate that the isolate represents a novel species of the genus Amycolatopsis. The proposed name is Amycolatopsis salitolerans sp. nov., with TRM F103T ( = JCM 15899T = CCTCC AB 208326T) as the type strain.

scholarly journals Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae

2011 ◽  
Vol 61 (3) ◽  
pp. 592-597 ◽  
Author(s):  
Yu-Qin Zhang ◽  
Hong-Yu Liu ◽  
Li-Yan Yu ◽  
Jae-Chan Lee ◽  
Dong-Jin Park ◽  
...  
Keyword(s):  
New Genus ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
West China ◽  
Qinghai Province ◽  
North West ◽  
The Family ◽  
Distinct Lineage ◽  
Diamino Acid

A Gram-positive, aerobic, non-motile actinobacterium, designated strain 6014T, was isolated from a soil sample collected from Qinghai province, north-west China, and subjected to a polyphasic taxonomic study. The isolate formed elementary branching hyphae and abundant aerial mycelia with globose sporangia on ISP 4 and R2A media. Whole-cell hydrolysates of strain 6014T contained arabinose, galactose and ribose as diagnostic sugars and meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids consisted of phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, two unknown phospholipids and an unknown glycolipid. The menaquinone system contained MK-9(H2) and MK-9(H4). The major fatty acids were C14 : 0, i-C15 : 0, C16 : 0 and 10-methyl-C16 : 1. The genomic DNA G+C content of the isolate was 69.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6014T fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to genera of the family Streptosporangiaceae. Based on data from this polyphasic study, strain 6014T can be readily distinguished from previously described organisms and represents a member of a novel species within a new genus in the suborder Streptosporangineae. The name Sinosporangium album gen. nov., sp. nov. is proposed with 6014T (=DSM 45181T =KCTC 19655T) as the type strain.

scholarly journals Streptomyces tailanensis sp. nov., an actinomycete isolated from riverside silt

2020 ◽  
Vol 70 (4) ◽  
pp. 2760-2765 ◽  
Author(s):  
Beibei Sun ◽  
Zhanfeng Xia ◽  
Linlin Yuan ◽  
Chuanxing Wan ◽  
Lili Zhang
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
West China ◽  
Content Type ◽  
North West ◽  
Link Type

A novel actinobacterium, designated TRM68348T, was isolated from the silt collected from the Tailan River in Xinjiang Province, north-west China. The strain was aerobic and Gram-stain-positive. The aerial mycelium was densely straight or tortuous, with a few branches of hyphae and no spores. The whole-cell sugar pattern of strain TRM68348T consisted of ribose and glucose. The diagnostic diamino was ll-diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannose and an unidentified phospholipid. The predominant menaquinones were MK-9 (H10), MK-9 (H6) and MK-9 (H2). The major fatty acids (>5 %) were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and summed feature 6. The G+C content of the genomic DNA was 69.93 mol%. Phylogenetic analysis showed that strain TRM68348T shared 16S rRNA gene sequence similarity of 98.14 % to the closest described species Streptomyces capitiformicae 1H-SSA4T. Strain TRM68348T had a relatively low DNA–DNA relatedness value with S. capitiformicae 1H-SSA4T as determined by calculating the average nucleotide identity value (92.78 %). Strain TRM68348T could also be differentiated from S. capitiformicae 1H-SSA4T based on morphological and physiological characteristics. On the basis of the evidence from this polyphasic study, the strain is concluded to represent a novel species of the genus Streptomyces , for which the name Streptomyces tailanensis sp. nov. is proposed. The type strain is TRM68348T (=CCTCC AA 2018086T=KCTC 49274T).

scholarly journals Pseudonocardia kunmingensis sp. nov., an actinobacterium isolated from surface-sterilized roots of Artemisia annua L.

2011 ◽  
Vol 61 (9) ◽  
pp. 2292-2297 ◽  
Author(s):  
Guo-Zhen Zhao ◽  
Jie Li ◽  
Hai-Yu Huang ◽  
Wen-Yong Zhu ◽  
Dong-Jin Park ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Artemisia Annua ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
West China ◽  
Sequence Similarities

A Gram-positive, aerobic, actinobacterial strain with rod-shaped spores, designated YIM 63158T, was isolated from the surface-sterilized roots of Artemisia annua L. collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63158T belonged to the genus Pseudonocardia. The closest neighbours were ‘Pseudonocardia sichuanensis’ KLBMP 1115 (99.9 % 16S rRNA gene sequence similarity), Pseudonocardia adelaidensis EUM 221T (99.1 %) and Pseudonocardia zijingensis DSM 44774T (98.8 %); sequence similarities to other members of the genus Pseudonocardia ranged from 98.6 to 94.4 %. The chemotaxonomic characteristics, such as the cell-wall diaminopimelic acid, whole-cell sugars, fatty acid components and major menaquinones, suggested that the isolate belonged to the genus Pseudonocardia. The G+C content of the genomic DNA was 73.3 mol%. On the basis of physiological, biochemical and chemotaxonomic data, including low DNA–DNA relatedness between the isolate and other members of the genus Pseudonocardia, it is proposed that strain YIM 63158T represents a novel species in this genus, with the name Pseudonocardia kunmingensis sp. nov. The type strain is YIM 63158T ( = DSM 45301T  = CCTCC AA 208081T).

scholarly journals Paraglycomyces xinjiangensis gen. nov., sp. nov., a halophilic actinomycete

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 4263-4269 ◽  
Author(s):  
Xiao-Xia Luo ◽  
Xiao-Xue Han ◽  
Fei Zhang ◽  
Chuan-Xing Wan ◽  
Li-Li Zhang
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
West China ◽  
North West ◽  
The Family ◽  
Nacl Concentration ◽  
Halophilic Actinomycete ◽  
Sequence Similarities

A novel halophilic actinobacterium, designated strain TRM 49201T, was isolated from a hypersaline soil in Xinjiang Province, north-west China. The strain was aerobic, Gram-stain-positive and halophilic. The aerial mycelium was chaotic with irregular branches, and spherical sporangia containing several spherical spores developed at mycelial aggregations. The strain had an optimum NaCl concentration for growth of 8–13 % (w/v). The whole-cell sugar pattern of strain TRM 49201T consisted of xylose and ribose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-10(H2). The polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylglycerol, phosphatidylcholine and four unknown phospholipids. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 70 mol%. Phylogenetic analysis showed that strain TRM 49201T can be distinguished from representatives of Glycomyces, Stackebrandtia and Haloglycomyces, the three existing genera in the family Glycomycetaceae, based on low 16S rRNA gene sequence similarities ( < 94.42 %). Strain TRM 49201T is thus considered to represent a novel species of a new genus in the family Glycomycetaceae, for which the name Paraglycomyces xinjiangensis gen. nov., sp. nov. is proposed. The type strain of Paraglycomyces xinjiangensis is TRM 49201T ( = NRRL B-24926T = CCTCC AA 2013002T = KACC 17683T).

Longimycelium tulufanense gen. nov., sp. nov., a filamentous actinomycete of the family Pseudonocardiaceae

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 2813-2818 ◽  
Author(s):  
Zhan-Feng Xia ◽  
Tong-Wei Guan ◽  
Ji-Sheng Ruan ◽  
Ying Huang ◽  
Li-Li Zhang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
North West ◽  
Link Type ◽  
The Family

A novel filamentous actinomycete strain, designated TRM 46004T, was isolated from sediment of Aiding Lake in Tulufan Basin (42° 64′ N 89° 26′ E), north-west China. The isolate was characterized using a polyphasic approach. The isolate formed abundant aerial mycelium with few branches and vegetative mycelium, occasionally twisted and coiled; spherical sporangia containing one to several spherical spores developed at the ends of short sporangiophores on aerial mycelium. The G+C content of the DNA was 65.2 mol%. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and xylose, galactose and ribose as the major whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H10). The major fatty acids were iso-C16 : 0 and anteiso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 46004T formed a distinct lineage within the family Pseudonocardiaceae and showed 91.7–96.1 % 16S rRNA gene sequence similarity with members of the family Pseudonocardiaceae . On the basis of the evidence from this polyphasic study, a novel genus and species, Longimycelium tulufanense gen. nov., sp. nov., are proposed. The type strain of Longimycelium tulufanense is TRM 46004T ( = CGMCC 4.5737T = NBRC 107726T).

scholarly journals Bacillus galliciensis sp. nov., isolated from faeces of wild seahorses (Hippocampus guttulatus)

2010 ◽  
Vol 60 (4) ◽  
pp. 892-895 ◽  
Author(s):  
José Luis Balcázar ◽  
José Pintado ◽  
Miquel Planas
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Positive Staining ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
North West ◽  
Hippocampus Guttulatus

A Gram-positive-staining, motile, rod-shaped, endospore-forming bacterium (BFLP-1T) was isolated from faeces of wild long-snouted seahorses (Hippocampus guttulatus) captured in north-west Spain (Toralla, Galicia). Strain BFLP-1T grew at 10–30 °C and pH 5.5–9 (optimally at 20 °C and pH 7.2) and with 0–7 % (w/v) NaCl (optimally with 2 % NaCl). The G+C content of the DNA was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BFLP-1T was a member of the genus Bacillus and was most closely related to Bacillus herbersteinensis D-1,5aT (96.6 %), B. shackletonii LMG 18435T (96.0 %) and B. isabeliae CVS-8T (95.9 %). Chemotaxonomic data (peptidoglycan type, meso-diaminopimelic acid; major menaquinone, MK-7; predominant fatty acids, anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1 ω11c; major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminoglycophospholipid) supported the affiliation of strain BFLP-1T to the genus Bacillus. Comparative analysis of 16S rRNA gene sequences and chemotaxonomic and phenotypic features indicated that strain BFLP-1T represents a novel species within the genus Bacillus, for which the name Bacillus galliciensis sp. nov. is proposed. The type strain is BFLP-1T (=DSM 21539T =LMG 24668T).

Streptomyces aidingensis sp. nov., an actinomycete isolated from lake sediment

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3204-3208 ◽  
Author(s):  
Zhan-Feng Xia ◽  
Ji-Sheng Ruan ◽  
Ying Huang ◽  
Li-Li Zhang
Keyword(s):  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
North West ◽  
Link Type ◽  
Gram Staining ◽  
Actinomycete Strain

A novel actinomycete strain, designated TRM 46012T, was isolated from sediment of Aiding Lake in Tulufan Basin (42° 64′ N 89° 26′ E), north-west China. The strain was aerobic and Gram-staining-positive with an optimum NaCl concentration for growth of 0–5 % (w/v). The isolate had sparse aerial mycelium and produced bud-shaped spores at the end of the aerial mycelium on ISP medium 4. The isolate contained ll-diaminopimelic acid as the diagnostic diamino acid and ribose as the major whole-cell sugar. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified phospholipid and three unidentified glycolipids. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The major fatty acids were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0. The G+C content of the DNA was 74.4 mol%. Phylogenetic analysis showed that strain TRM 46012T had 16S rRNA gene sequence similarity of 95.7 % with the most closely related species with a validly published name, Streptomyces cheonanensis , and it could be distinguished from all species in the genus Streptomyces by using the data from this polyphasic taxonomic study. On the basis of these data, strain TRM 46012T should be designated as a representative of a novel species of the genus Streptomyces , for which the name Streptomyces aidingensis sp. nov. is proposed. The type strain is TRM 46012T ( = CGMCC 4.5739T = NBRC 108211T).

scholarly journals Actinomadura alba sp. nov., isolated from soil in Yunnan, China

2007 ◽  
Vol 57 (8) ◽  
pp. 1735-1739 ◽  
Author(s):  
Yong-Xia Wang ◽  
Xiao-Yang Zhi ◽  
Hua-Hong Chen ◽  
Yu-Qin Zhang ◽  
Shu-Kun Tang ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Genetic Data ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
The Family ◽  
Evolutionary Radiation ◽  
Actinomycete Strain

A novel actinomycete, strain YIM 45681T, which was isolated from soil in a suburb of Kunming, Yunnan Province, China, was subjected to phenotypic and genotypic characterization. The micro-organism, which produces short spore chains arranged in spirals on the aerial mycelium, was shown to have meso-diaminopimelic acid in the cell wall. The sugars present in whole-cell hydrolysates were ribose, xylose, galactose, madurose and glucose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The phospholipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C16 : 1 H, C17 : 1 ω8c, 10-methyl C17 : 0 and C16 : 0. A comparative analysis of 16S rRNA gene sequences indicated that the organism formed a distinct clade within the evolutionary radiation of the family Thermomonosporaceae, and that it was closely associated with members of the genus Actinomadura. A broad range of phenotypic and genetic data supported the suggestion that this organism represents a novel species of the genus Actinomadura, for which the name Actinomadura alba sp. nov. is proposed, with YIM 45681T (=DSM 45045T =CCTCC AA206005T) as the type strain.

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