Bacillus thermotolerans sp. nov., a thermophilic bacterium capable of reducing humus

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3672-3678 ◽  
Author(s):  
Guiqin Yang ◽  
Xuemei Zhou ◽  
Shungui Zhou ◽  
Dehui Yang ◽  
Yueqiang Wang ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Cellular Fatty Acid ◽  
Thermophilic Bacterium ◽  
Anaerobic Growth ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
Gram Staining

A novel thermotolerant bacterium, designated SgZ-8T, was isolated from a compost sample. Cells were non-motile, endospore-forming, Gram-staining positive, oxidase-negative and catalase-positive. The isolate was able to grow at 20–65 °C (optimum 50 °C) and pH 6.0–9.0 (optimum 6.5–7.0), and tolerate up to 9.0 % NaCl (w/v) under aerobic conditions. Anaerobic growth occurred with anthraquinone-2,6-disulphonate (AQDS), fumarate and NO3 - as electron acceptors. Phylogenetic analysis based on the16S rRNA and gyrB genes grouped strain SgZ-8T into the genus Bacillus , with the highest similarity to Bacillus badius JCM 12228T (96.2 % for 16S rRNA gene sequence and 83.5 % for gyrB gene sequence) among all recognized species in the genus Bacillus . The G+C content of the genomic DNA was 49.3 mol%. The major isoprenoid quinone was menaquinone 7 (MK-7) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major cellular fatty acid was iso-C16 : 0. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-8T ( = CCTCC AB 2012108T = KACC 16706T) was designated the type strain of a novel species of the genus Bacillus , for which the name Bacillus thermotolerans sp. nov. is proposed.

Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 867-872 ◽  
Author(s):  
Herbert Seiler ◽  
Mareike Wenning ◽  
Verena Schmidt ◽  
Siegfried Scherer
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Acid Type

A novel Gram-staining-positive, rod-shaped, motile, strictly aerobic, endospore-forming bacterium, designated WCC 4585T, was isolated from a pharmaceutical production line. The organism grew optimally at 30 °C, at pH 8 and in the presence of 0.5 % (w/v) NaCl. Oval endospores were formed subterminally and terminally in swollen sporangia. The cell-wall diamino acid was meso-diaminopimelic acid (type A1γ) and the genomic DNA G+C content was 38.7 mol%. The major menaquinone was MK-7. The cellular fatty acid profile contained major amounts of iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, and the cellular phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. The isolate was most closely related to Bacillus oceanisediminis H2T, Bacillus infantis SMC 4352-1T, Bacillus firmus NCIMB 9366T, Bacillus circulans ATCC 4513T and Bacillus horneckiae DSM 23495T with which it shared less than 98.0 % 16S rRNA gene sequence similarity. DNA–DNA relatedness values between strain WCC 4585T and five type strains of related species were ≤27 % and sequence similarity values based on groEL sequences were ≤88.7 %. On the basis of the characteristics presented, strain WCC 4585T is proposed to represent a novel species, Bacillus gottheilii sp. nov. The type strain is WCC 4585T( = DSM 23668T = CCUG 59876T = LMG 25856T).

Arcanobacterium phocisimile sp. nov., isolated from harbour seals

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2019-2024 ◽  
Author(s):  
Muaz Hijazin ◽  
Osama Sammra ◽  
Hivda Ülbegi-Mohyla ◽  
Samy Nagib ◽  
Jörg Alber ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
Harbour Seals

A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium -like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370T (98.4 % 16S rRNA gene sequence similarity), A. canis P6775T (97.4 %), A. phocae DSM 10002T (97.4 %), A. pluranimalium M430/94/2T (95.7 %) and A. hippocoleae CCUG 44697T (95.5 %). The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolates with the genus Arcanobacterium . The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C16 : 0, C18 : 0, C18 : 1ω9c and summed feature 5 (comprising C18 : 2ω6,9c and/or anteiso-C18 : 0). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium . Based on the common origin and various physiological properties comparable to those of A. phocae , it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698T ( = LMG 27073T  = CCM 8430T).

scholarly journals Polaribacter atrinae sp. nov., isolated from the intestine of a comb pen shell, Atrina pectinata

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1654-1661 ◽  
Author(s):  
Dong-Wook Hyun ◽  
Na-Ri Shin ◽  
Min-Soo Kim ◽  
Pil Soo Kim ◽  
Mi-Ja Jung ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Pen Shell ◽  
Atrina Pectinata

A novel Gram-staining-negative, aerobic, non-motile, yellow-to-orange carotenoid-type-pigmented and rod-shaped bacterium, designated strain WP25T, was isolated from the intestine of a comb pen shell, Atrina pectinata, which was collected from the South Sea near Yeosu in Korea. The isolate grew optimally at 20 °C, at pH 7 and with 2 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain WP25T belonged to the genus Polaribacter in the family Flavobacteriaceae and the highest sequence similarity was shared with the type strain of Polaribacter sejongensis (98.5 %). The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, C15 : 1ω6c and iso-C15 : 0 3-OH. The main respiratory quinone was menaquinone MK-6. The polar lipids of strain WP25T were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and four unidentified lipids. The genomic DNA G+C content was 31.2 mol%. DNA–DNA hybridization experiments indicated <12.6 % genomic relatedness with closely related strains. Based on phylogenetic, phenotypic and genotypic analyses, strain WP25T represents a novel species in the genus Polaribacter , for which the name Polaribacter atrinae sp. nov. is proposed, with the type strain WP25T ( = KACC 17473T = JCM 19202T).

Taibaiella koreensis sp. nov., isolated from soil of a ginseng field

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 1018-1023 ◽  
Author(s):  
Heung-Min Son ◽  
MooChang Kook ◽  
Ju-Han Kim ◽  
Tae-Hoo Yi
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Biochemical Tests ◽  
Phenotypic Data ◽  
Link Type ◽  
Gram Staining ◽  
The Republic

A Gram-staining-negative, strictly aerobic, motile (by gliding), non-spore-forming and rod-shaped bacterial strain, designated THG-DT86T, was isolated from soil of a ginseng field of Pocheon province in the Republic of Korea and its taxonomic position was investigated by a polyphasic approach. Growth occurred at 10–35 °C, at pH 6.5–8.5 and with 0–1.5 % (w/v) NaCl on trypticase soy agar. Flexirubin-type pigments were found to be present. On the basis of 16S rRNA gene sequence similarity, strain THG-DT86T was shown to belong to the genus Taibaiella and was related to Taibaiella smilacinae PTJT-5T (95.3 %). The G+C content of the genomic DNA was 50.1 mol%. The only isoprenoid quinone detected in strain THG-DT86T was menaquinone-7 (MK-7) and the only polyamine was homospermidine. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0, iso-C15 : 1 G and iso-C17 : 0, and the major polar lipids were phosphatidylethanolamine, an unidentified aminophosphoglycolipid and an unidentified aminophospholipid. Phenotypic data and phylogenetic inference supported the affiliation of strain THG-DT86T to the genus Taibaiella , and a number of biochemical tests differentiated strain THG-DT86T from the recognized species of the genus Taibaiella . Therefore, the novel isolate represents a novel species, for which the name Taibaiella koreensis sp. nov. is proposed, with THG-DT86T as the type strain ( = KACC 17171T = JCM 18823T).

scholarly journals Pedobacter nutrimenti sp. nov., isolated from chilled food

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1310-1316 ◽  
Author(s):  
Julia Derichs ◽  
Peter Kämpfer ◽  
André Lipski
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated J22T, was isolated from fresh pasta filled with pork (tortellini) that had been stored at 6 °C. Based on 16S rRNA gene sequence analysis, strain J22T falls within the radiation of species of the genus Pedobacter . On the basis of 16S rRNA gene sequence similarities, the closest species phylogenetically are Pedobacter panaciterrae LMG 23400T (98.7 %), Pedobacter africanus DSM 12126T (98.5 %) and Pedobacter heparinus DSM 2366T (98.3 %). The only isoprenoid quinone of strain J22T was menaquinone 7 (MK-7). The major fatty acids were iso-C15 : 0 (34.8 %), C16 : 1 cis9 (24.9 %), C16 : 0 (7.3 %), C14 : 0 (4.8 %) and iso-C17 : 0 3-OH (4.4 %). These chemotaxonomic characteristics supported the assignment of the isolate to the genus Pedobacter . DNA–DNA hybridization experiments showed that the DNA–DNA relatedness between strain J22T and its closest phylogenetic neighbour, Pedobacter panaciterrae LMG 23400T, was less than 36 %. This indicated that the isolate was distinct from this species. Biochemical tests allowed strain J22T to be phenotypically differentiated from closely related species of the genus Pedobacter . As a consequence, strain J22T should be classified as a representative of a novel species of the genus Pedobacter for which the name Pedobacter nutrimenti sp. nov. is proposed. The type strain is J22T ( = DSM 27372T = CCUG 64422T).

scholarly journals Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier’s gangrene

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3140-3145 ◽  
Author(s):  
Piet Cools ◽  
Matthijs Oyaert ◽  
Mario Vaneechoutte ◽  
Emmanuel De Laere ◽  
Steven Vervaeke
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Fournier’S Gangrene ◽  
Rrna Gene ◽  
Fournier's Gangrene ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type

A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.

scholarly journals Streptomyces graminifolii sp. nov., isolated from bamboo (Sasa borealis) litter

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2517-2521 ◽  
Author(s):  
Hyo-Jin Lee ◽  
Kyung-Sook Whang
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

The taxonomic position of strain JL-22T, isolated from litter of a bamboo (Sasa borealis) forest, was determined using a polyphasic approach. The organism had phenotypic and morphological properties consistent with it being a member of the genus Streptomyces . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain JL-22T was closely related to Streptomyces prunicolor NRRL B-12281T (99.2 %), Streptomyces galilaeus JCM 4757T (99.0 %) and Streptomyces chartreusis NBRC 12753T (99.0 %). However, the results of DNA–DNA hybridization and physiological and biochemical tests showed that strain JL-22T could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Based on phenotypic and genotypic data, strain JL-22T represents a novel species of the genus Streptomyces , for which the name Streptomyces graminifolii sp. nov. is proposed. The type strain is JL-22T ( = KACC 17180T = NBRC 109806T).

Chitinophaga cymbidii sp. nov., isolated from Cymbidium goeringii roots

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1800-1804 ◽  
Author(s):  
Lubin Li ◽  
Lei Sun ◽  
Nan Shi ◽  
Lin Liu ◽  
Huijuan Guo ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Gram Staining ◽  
Cymbidium Goeringii

Two Gram-staining-negative, aerobic, non-spore-forming rod-shaped, non-motile bacteria, designated strains R156-2T and T58-2 were isolated from the roots of Cymbidium goeringii. The colonies were yellow-pigmented. On the basis of 16S rRNA gene sequence similarity, strains R156-2T and T58-2 were shown to be members of the genus Chitinophaga . Strains R156-2T and T58-2 showed the greatest level of sequence similarity with Chitinophaga niabensis (96.0–96.3 %). The major menaquinone was MK-7. The main cellular fatty acids were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. Phenotypic and genotypic analyses indicated that strains R156-2T and T58-2 could not be assigned to any recognized species. Therefore, strains R156-2T and T58-2 represent a novel species of the genus Chitinophaga , for which the name Chitinophaga cymbidii sp. nov. is proposed. The type strain is R156-2T ( = ACCC 05363T = KCTC 23738T). The DNA G+C content of this strain is 51.9 mol%.

scholarly journals Janibacter indicus sp. nov., isolated from hydrothermal sediment of the Indian Ocean

2014 ◽  
Vol 64 (Pt_7) ◽  
pp. 2353-2357 ◽  
Author(s):  
Gaiyun Zhang ◽  
Huihui Ren ◽  
Shuang Wang ◽  
Xiu Chen ◽  
Yanliu Yang ◽  
...  
Keyword(s):  
Indian Ocean ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Hydrothermal Sediment ◽  
Diamino Acid ◽  
The Indian Ocean

A Gram-staining-positive, aerobic and non-motile strain, 0704P10-1T, was isolated from hydrothermal sediment of the Indian Ocean. Phylogenetic, phenotypic and chemotaxonomic data for the organism supported that it belonged to the genus Janibacter . Strain 0704P10-1T showed 97.2–98.7 % 16S rRNA gene sequence similarities to the type strains of recognized members of the genus Janibacter . It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall. MK-8(H4) was the only menaquinone detected. The major fatty acids were iso-C16 : 0, C17 : 1ω8c and 10-methyl C17 : 0. Meanwhile, the results of DNA–DNA hybridization studies and other physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain 0704P10-1T from closely related species. Thus, strain 0704P10-1T represents a novel species of the genus Janibacter , for which the name Janibacter indicus sp. nov. is proposed. The type strain is 0704P10-1T ( = LMG 27493T = CGMCC 1.12511T).

Mucilaginibacter inviolabilis sp. nov., isolated from the phycosphere of Haematococcus lacustris NIES 144 culture

2021 ◽  
Author(s):  
Sang-Ah Lee ◽  
Ve Van Le ◽  
So-Ra Ko ◽  
Nakyeong Lee ◽  
Hee-Mock Oh ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Haematococcus Lacustris ◽  
The 16S Rrna Gene

A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterial strain, designated HC2T, was isolated from the phycosphere of Haematococcus lacustris NIES 144 culture. Strain HC2T was able to grow at pH 4.5–8.0, at 4–32 °C and in the presence of 0–2 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain HC2T was affiliated to the genus Mucilaginibacter and shared the highest sequence similarity with Mucilaginibacter lappiensis ANJKI2T (98.20 %) and Mucilaginibacter sabulilitoris SMS-12T (98.06 %). Strain HC2T contained summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C15 : 0 as the major fatty acids (>10.0 %). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. The respiratory quinone was menaquinone 7 (MK-7). The genomic DNA G+C content was 42.0 %. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain HC2T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter inviolabilis sp. nov. is proposed. The type strain is HC2T (=KCTC 82084T=JCM 34116T).

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