Identification of the IFN-β response in H3N2 canine influenza virus infection

Canine parvovirus (CPV) and canine influenza virus (CIV) are highly contagious virus infecting members of the canine family including dogs, coyotes, foxes and wolves. The research work was carried out to determine the prevalence of CPV and CIV in dogs (stray and pet dogs) in Dhaka, Mymensingh, Feni and Chittagong Districts by using Rapigen CPV and CIV Ag test kit. Fecal samples were collected from 50 dogs of different age and sex from different spots of Dhaka and Chittagong District. Nasal fluid samples were collected from 50 dogs of different age and sex from different spots. The samples were tested with Rapigen canine parvovirus Ag test kit and Rapigen canine influenza Ag test kit. Overall prevalence of CPV in pet dogs was 22% and in stray dogs was 30%. The prevalence of canine parvovirus in relation to age was gradually decreasing with higher age group and the prevalence was 28% in 1-6 months age, 16.66% in 7-12 months age group 11.11%o ver 18 months age group. The prevalence of canine parvovirus was significantly higher in male (24.13%) than that in female (19.04%). All samples showed negative test result for canine influenza virus. Prevalence of canine influenza virus infection were 00.00% in all age groups and sex. Overall prevalence of CIV in all pet and stray dogs were 00.00%.Asian J. Med. Biol. Res. March 2016, 2(1): 138-142

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Serological evidence of H3N2 canine influenza virus infection among horses with dog exposure

Transboundary and Emerging Diseases ◽

10.1111/tbed.13104 ◽

2019 ◽

Vol 66 (2) ◽

pp. 915-920

Author(s):

Pei Zhou ◽

Aijian Luo ◽

Xiangyu Xiao ◽

Xue Hu ◽

Dan Shen ◽

...

Keyword(s):

Influenza Virus ◽

Virus Infection ◽

Influenza Virus Infection ◽

Serological Evidence ◽

Canine Influenza Virus ◽

Canine Influenza

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Serological evidence of canine influenza virus infection in shelter dogs in Turkey

Medycyna Weterynaryjna ◽

10.21521/mw.5986 ◽

2018 ◽

Vol 74 (6) ◽

pp. 5986-2018 ◽

Cited By ~ 1

Author(s):

HAKAN AYDIN ◽

AKIN KIRBAS ◽

MEHMET OZKAN TIMURKAN ◽

MUSTAFA SINAN AKTAS ◽

GULIZAR ACAR KIRMIZI ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Influenza Virus ◽

Virus Infection ◽

Reverse Transcriptase ◽

Influenza Virus Infection ◽

Enzyme Linked Immunosorbent Assay ◽

Chain Reaction ◽

Canine Influenza Virus ◽

Polymerase Chain ◽

Canine Influenza

Influenza virus infection is an important disease which occurs in humans and a variety of animals. Because of the wide host adaptation and segmented genome, there is always the possibility of mutations and interspecies transmission of the influenza virus. Our study is the first to draw attention to canine influenza infection in Turkey. For this purpose, 208 sera and swab samples were collected from dogs with respiratory and nonrespiratory signs in various seasons. Out of the 208 dogs, 94 (45.2%) were male and 114 (54.8%) were female; the average age was 4.7 years. A total of 208 sera samples were tested for the presence of canine influenza virusspecific antibodies by the indirect enzyme-linked immunosorbent assay. The seroprevalence of canine influenza virus infection was 11/208 (5.8%). With regard to seasonal distribution, the highest rate of seropositivity was detected in spring, and the lowest in summer. Molecular detection of the canine influenza virus from nasal swab samples was done by reverse transcriptase polymerase chain reaction using specific universal primers for the hemagglutinin gene. Influenza virus nucleic acid could not be detected by reverse transcriptase polymerase chain reaction. In this study, we revealed for the first time the existence of the canine influenza virus in Turkey. Although the seroprevalence was relatively low, it would be useful to investigate the canine influenza virus on a large scale and among dogs with infectious respiratory disease in the Turkish dog population..

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Seroprevalence of avian origin H3N2 canine influenza virus infection in pet dogs in Shenzhen, China

African Journal of Microbiology Research ◽

10.5897/ajmr11.1082 ◽

2011 ◽

Vol 5 (32) ◽

Cited By ~ 1

Author(s):

Fu-Rong Zhao

Keyword(s):

Influenza Virus ◽

Virus Infection ◽

Influenza Virus Infection ◽

Pet Dogs ◽

Canine Influenza Virus ◽

Avian Origin ◽

Canine Influenza

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Evidence of H3N2 canine influenza virus infection before 2007

Veterinary Record ◽

10.1136/vr.100718 ◽

2012 ◽

Vol 171 (19) ◽

pp. 477.1-477 ◽

Cited By ~ 11

Author(s):

Y-N Lee ◽

D-H Lee ◽

H-J J Lee ◽

J-K Park ◽

S-S Yuk ◽

...

Keyword(s):

Influenza Virus ◽

Virus Infection ◽

Influenza Virus Infection ◽

Canine Influenza Virus ◽

Canine Influenza

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Integrated Lung and Tracheal mRNA-Seq and miRNA-Seq Analysis of Dogs with an Avian-Like H5N1 Canine Influenza Virus Infection

Frontiers in Microbiology ◽

10.3389/fmicb.2018.00303 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 8

Author(s):

Cheng Fu ◽

Jie Luo ◽

Shaotang Ye ◽

Ziguo Yuan ◽

Shoujun Li

Keyword(s):

Influenza Virus ◽

Virus Infection ◽

Influenza Virus Infection ◽

Canine Influenza Virus ◽

Canine Influenza

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H3N2 Canine Influenza Virus Infection in a Dog

Veterinary Pathology ◽

10.1177/0300985816681411 ◽

2016 ◽

Vol 54 (3) ◽

pp. 527-530 ◽

Cited By ~ 8

Author(s):

C. E. Watson ◽

C. Bell ◽

K. Toohey-Kurth

Keyword(s):

Influenza Virus ◽

Virus Infection ◽

Influenza Virus Infection ◽

Canine Influenza Virus ◽

Canine Influenza

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Role of CARD Region of MDA5 Gene in Canine Influenza Virus Infection

Viruses ◽

10.3390/v12030307 ◽

2020 ◽

Vol 12 (3) ◽

pp. 307 ◽

Cited By ~ 1

Author(s):

Cheng Fu ◽

Shaotang Ye ◽

Yongbo Liu ◽

Shoujun Li

Keyword(s):

Innate Immunity ◽

Influenza Virus ◽

Cytokine Production ◽

Influenza Virus Infection ◽

Luciferase Assay ◽

Canine Influenza Virus ◽

Card Domain ◽

Canine Influenza ◽

Receptor Family

MDA5 belongs to the RIG-I-like receptor family, which is involved in innate immunity. During viral infection, MDA5 generates an antiviral response by recognizing the ligand to activate interferon. However, the role and mechanism of MDA5 in canine influenza virus (CIV) infection are unclear. To understand the mechanism of canine MDA5-mediated innate immunity during CIV infection, we detected the distribution of MDA5 in beagles, and the structural prediction showed that MDA5 was mainly composed of a CARD domain, RD domain, and DExD/H helix structure. Moreover, we found that MDA5 inhibits CIV replication. Furthermore, in the dual luciferase assay, we revealed that the CARD region of MDA5 strongly activated the IFN-β promoter and mainly transmitted signals through the CARD region. Overexpression of the CARD region of MDA5 revealed that the MDA5-mediated signaling pathway could transmit signals by activating the IRF3/NF-κB and IRF3 promoters, promoting the expression of antiviral proteins and cytokine release, thereby inhibiting CIV replication. Upon silencing of MDA5, cytokine production decreased, while the replication ability of CIV was increased. Thus, this study revealed a novel mechanism by which MDA5 mediated CIV infection and provided new avenues for the development of antiviral strategies.

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Phosphoproteomics to Characterize Host Response During H3N2 Canine Influenza Virus Infection of Dog Lung

Frontiers in Veterinary Science ◽

10.3389/fvets.2020.585071 ◽

2020 ◽

Vol 7 ◽

Author(s):

Yongbo Liu ◽

Cheng Fu ◽

Shaotang Ye ◽

Yingxin Liang ◽

Zhonghe Qi ◽

...

Keyword(s):

Virus Infection ◽

Influenza Virus Infection ◽

Enrichment Analysis ◽

Influenza Viruses ◽

Host Protein ◽

Differentially Expressed ◽

H3n2 Virus ◽

Post Inoculation ◽

Canine Influenza Virus ◽

Canine Influenza

Avian-origin H3N2 canine influenza viruses (CIVs) cause severe contagious respiratory disease in dogs, and quickly adapt to new environments. To further understand the mechanism of virus infection and host-virus interactions, we characterized the complete phosphoproteome of dogs infected with H3N2 CIV. Nine-week-old Beagle dogs were inoculated intranasally with 106 EID50 of A/canine/Guangdong/04/2014 (H3N2) virus. Lung sections were harvested at 5 days post-inoculation (dpi) and processed for global and quantitative analysis of differentially expressed phosphoproteins. A total of 1,235 differentially expressed phosphorylated proteins were identified in the dog lung after H3N2 CIV infection, and 3,016 modification sites were identified among all differentially expressed proteins. We then performed an enrichment analysis of functional annotations using Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) database analyses to predict the functions of the identified differential phosphoproteins. Our data indicate that H3N2 CIV infection causes dramatic changes in the host protein phosphorylation of dog lungs. To our knowledge, this is the first study to assess the effect of H3N2 CIV infection on the phosphoproteome of beagles. These data provide novel insights into H3N2-CIV-triggered regulatory phosphorylation circuits and signaling networks and may improve our understanding of the mechanisms underlying CIV pathogenesis in dogs.

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