scholarly journals Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens

2018 ◽  
Vol 67 (11) ◽  
pp. 1576-1580 ◽  
Author(s):  
Yolanda I. I. Ho ◽  
Ann H. Wong ◽  
Raymond W. M. Lai
Keyword(s):  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Influenza A ◽  
Molecular Detection ◽  
Influenza B ◽  
Rt Pcr ◽  
Syncytial Virus ◽  
Respiratory Specimens

scholarly journals Comparative evaluation of real-time PCR and conventional RT-PCR during a 2 year surveillance for influenza and respiratory syncytial virus among children with acute respiratory infections in Kolkata, India, reveals a distinct seasonality of infection

2009 ◽  
Vol 58 (12) ◽  
pp. 1616-1622 ◽  
Author(s):  
Anurodh S. Agrawal ◽  
Mehuli Sarkar ◽  
Sekhar Chakrabarti ◽  
K. Rajendran ◽  
Harpreet Kaur ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Influenza A ◽  
Clinical Symptoms ◽  
Influenza B Virus ◽  
Influenza B ◽  
Rt Pcr ◽  
Syncytial Virus ◽  
Tract Infections

Acute respiratory tract infections (ARTIs) are one of the most common causes of morbidity and mortality in young children worldwide. Influenza virus and respiratory syncytial virus (RSV) are the predominant aetiological agents during seasonal epidemics, and thus rapid and sensitive molecular tests for screening for such agents and timely identification of epidemics are required. This study compared real-time quantitative PCR (qPCR) with conventional RT-PCR for parallel identification of influenza A virus (IAV) or influenza B virus (IBV) and RSV. A total of 1091 respiratory samples was examined from children with suspected ARTIs between January 2007 and December 2008. Of these, 275 (25.21 %) were positive for either influenza or RSV by qPCR compared with 262 (24 .01%) positive by RT-PCR. Overall, IAV, IBV and RSV were detected in 121 (11.09 %), 59 (5.41 %) and 95 (8.71 %) samples, respectively. In spite of overlapping clinical symptoms, RSV and influenza virus showed distinct seasonal peaks. IAV correlated positively and RSV negatively with rainfall and temperature. No distinct seasonality was observed in IBV infections. This is, to the best of our knowledge, the first report of a systemic surveillance of respiratory viruses with seasonal correlation and prevalence rates from eastern India. This 2 year comparative analysis also confirmed the feasibility of using qPCR in developing countries, which will not only improve the scope for prevention of epidemics, but will also provide crucial epidemiological data from tropical regions.

scholarly journals Clinical Performance of the AllplexTM Respiratory Panel 1 Test Compared to SimplexaTM Flu A/B and RSV for Detection of Influenza Virus and Respiratory Syncytial Virus Infection Including Their Subtyping

2019 ◽  
Vol 28 (4) ◽  
pp. 380-386 ◽  
Author(s):  
Jeeyong Kim ◽  
Jeonghun Nam ◽  
Woongsik Jang ◽  
Chae Seung Lim
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Influenza A ◽  
Clinical Performance ◽  
Diagnostic Tools ◽  
Influenza B Virus ◽  
Influenza B ◽  
Rt Pcr ◽  
Predictive Values ◽  
Syncytial Virus

Objective: TheAllplexTM Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B), and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to the SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools. Materials and Methods: A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR. Results: The sensitivity of ARP was 95.56, 100, and 95.24% for Flu A, Flu B, and RSV, respectively. The specificity of ARP was 100, 100, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89 and 100% for Flu A, 100 and 100% for Flu B, and 100 and 100% for RSV. Conclusion: The Allplex panelshowed high sensitivity, specificity, positive predictive, and negative predictive values for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 min and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.

scholarly journals Monitoring epidemic viral respiratory infections using one-step real-Time Triplex RT-PCR targeting influenza A and B viruses and respiratory syncytial virus

2011 ◽  
Vol 83 (4) ◽  
pp. 695-701 ◽  
Author(s):  
Solesne Papillard-Marechal ◽  
Vincent Enouf ◽  
Aurélie Schnuriger ◽  
Astrid Vabret ◽  
Edouard Macheras ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Influenza A ◽  
Respiratory Infections ◽  
Rt Pcr ◽  
One Step ◽  
Syncytial Virus ◽  
Viral Respiratory Infections ◽  
Pcr Targeting ◽  
Viral Respiratory

scholarly journals A comparison of influenza and respiratory syncytial virus infections among infants admitted to hospital with acute respiratory infections

1976 ◽  
Vol 77 (3) ◽  
pp. 383-392 ◽  
Author(s):  
E. O. Caul ◽  
D. K. Waller ◽  
S. K. R. Clarke ◽  
B. D. Corner
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Rural Areas ◽  
Influenza A ◽  
Respiratory Infections ◽  
Influenza B Virus ◽  
Influenza B ◽  
Virus Infections ◽  
Syncytial Virus ◽  
One Year

SUMMARYAmong 741 children under 5 years admitted to hospital with respiratory infections during two winters, infection with influenza A virus was diagnosed in 70 (9%), with influenza B virus in 8 (1%), and with respiratory syncytial virus (RSV) in 259 (35 %). Both influenza virus and RSV infections were diagnosed most frequently in children under the age of one year, and diagnosed more frequently in males than females. Influenza illnesses were more severe in boys than girls. Both infections occurred more often, but were not more severe, in children from a conurbation than in those from ‘rural’ areas. Convulsions were the cause of 36% of admissions with influenza A infections, but were rare in RSV infections. Bronchiolitis was the reason for 39% of admissions with RSV infections, but was rare in influenza infections. It is suggested that infants admitted to hospital are a good source of influenza virus strains for monitoring arttigenic variation.

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