scholarly journals Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the 2009 H1N1 Swine-Origin Influenza Virus Epidemic in Milwaukee, Wisconsin

2010 ◽  
Vol 12 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Eric T. Beck ◽  
Lisa A. Jurgens ◽  
Sue C. Kehl ◽  
Michael E. Bose ◽  
Teresa Patitucci ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Influenza A ◽  
Influenza B ◽  
Rt Pcr ◽  
Pcr Assay ◽  
2009 H1n1 ◽  
Syncytial Virus

scholarly journals Comparative evaluation of real-time PCR and conventional RT-PCR during a 2 year surveillance for influenza and respiratory syncytial virus among children with acute respiratory infections in Kolkata, India, reveals a distinct seasonality of infection

2009 ◽  
Vol 58 (12) ◽  
pp. 1616-1622 ◽  
Author(s):  
Anurodh S. Agrawal ◽  
Mehuli Sarkar ◽  
Sekhar Chakrabarti ◽  
K. Rajendran ◽  
Harpreet Kaur ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Influenza A ◽  
Clinical Symptoms ◽  
Influenza B Virus ◽  
Influenza B ◽  
Rt Pcr ◽  
Syncytial Virus ◽  
Tract Infections

Acute respiratory tract infections (ARTIs) are one of the most common causes of morbidity and mortality in young children worldwide. Influenza virus and respiratory syncytial virus (RSV) are the predominant aetiological agents during seasonal epidemics, and thus rapid and sensitive molecular tests for screening for such agents and timely identification of epidemics are required. This study compared real-time quantitative PCR (qPCR) with conventional RT-PCR for parallel identification of influenza A virus (IAV) or influenza B virus (IBV) and RSV. A total of 1091 respiratory samples was examined from children with suspected ARTIs between January 2007 and December 2008. Of these, 275 (25.21 %) were positive for either influenza or RSV by qPCR compared with 262 (24 .01%) positive by RT-PCR. Overall, IAV, IBV and RSV were detected in 121 (11.09 %), 59 (5.41 %) and 95 (8.71 %) samples, respectively. In spite of overlapping clinical symptoms, RSV and influenza virus showed distinct seasonal peaks. IAV correlated positively and RSV negatively with rainfall and temperature. No distinct seasonality was observed in IBV infections. This is, to the best of our knowledge, the first report of a systemic surveillance of respiratory viruses with seasonal correlation and prevalence rates from eastern India. This 2 year comparative analysis also confirmed the feasibility of using qPCR in developing countries, which will not only improve the scope for prevention of epidemics, but will also provide crucial epidemiological data from tropical regions.

Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus

2010 ◽  
Vol 167 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Weston C. Hymas ◽  
Alan Mills ◽  
Sheri Ferguson ◽  
Janine Langer ◽  
Rosemary C. She ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Real Time ◽  
Influenza A ◽  
H1n1 Influenza ◽  
Influenza B ◽  
Rt Pcr ◽  
H1n1 Influenza Virus ◽  
Pcr Assay ◽  
2009 H1n1

scholarly journals Clinical Performance of the AllplexTM Respiratory Panel 1 Test Compared to SimplexaTM Flu A/B and RSV for Detection of Influenza Virus and Respiratory Syncytial Virus Infection Including Their Subtyping

2019 ◽  
Vol 28 (4) ◽  
pp. 380-386 ◽  
Author(s):  
Jeeyong Kim ◽  
Jeonghun Nam ◽  
Woongsik Jang ◽  
Chae Seung Lim
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Influenza A ◽  
Clinical Performance ◽  
Diagnostic Tools ◽  
Influenza B Virus ◽  
Influenza B ◽  
Rt Pcr ◽  
Predictive Values ◽  
Syncytial Virus

Objective: TheAllplexTM Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B), and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to the SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools. Materials and Methods: A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR. Results: The sensitivity of ARP was 95.56, 100, and 95.24% for Flu A, Flu B, and RSV, respectively. The specificity of ARP was 100, 100, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89 and 100% for Flu A, 100 and 100% for Flu B, and 100 and 100% for RSV. Conclusion: The Allplex panelshowed high sensitivity, specificity, positive predictive, and negative predictive values for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 min and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.

scholarly journals Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 234
Author(s):  
Sarah Al-Beltagi ◽  
Cristian Alexandru Preda ◽  
Leah V. Goulding ◽  
Joe James ◽  
Juan Pu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Influenza A Virus ◽  
Broad Spectrum ◽  
Influenza A ◽  
Respiratory Viruses ◽  
Influenza Viruses ◽  
Virus Challenge ◽  
2009 H1n1 ◽  
Syncytial Virus

The long-term control strategy of SARS-CoV-2 and other major respiratory viruses needs to include antivirals to treat acute infections, in addition to the judicious use of effective vaccines. Whilst COVID-19 vaccines are being rolled out for mass vaccination, the modest number of antivirals in use or development for any disease bears testament to the challenges of antiviral development. We recently showed that non-cytotoxic levels of thapsigargin (TG), an inhibitor of the sarcoplasmic/endoplasmic reticulum (ER) Ca2+ ATPase pump, induces a potent host innate immune antiviral response that blocks influenza A virus replication. Here we show that TG is also highly effective in blocking the replication of respiratory syncytial virus (RSV), common cold coronavirus OC43, SARS-CoV-2 and influenza A virus in immortalized or primary human cells. TG’s antiviral performance was significantly better than remdesivir and ribavirin in their respective inhibition of OC43 and RSV. Notably, TG was just as inhibitory to coronaviruses (OC43 and SARS-CoV-2) and influenza viruses (USSR H1N1 and pdm 2009 H1N1) in separate infections as in co-infections. Post-infection oral gavage of acid-stable TG protected mice against a lethal influenza virus challenge. Together with its ability to inhibit the different viruses before or during active infection, and with an antiviral duration of at least 48 h post-TG exposure, we propose that TG (or its derivatives) is a promising broad-spectrum inhibitor against SARS-CoV-2, OC43, RSV and influenza virus.

scholarly journals A comparison of influenza and respiratory syncytial virus infections among infants admitted to hospital with acute respiratory infections

1976 ◽  
Vol 77 (3) ◽  
pp. 383-392 ◽  
Author(s):  
E. O. Caul ◽  
D. K. Waller ◽  
S. K. R. Clarke ◽  
B. D. Corner
Keyword(s):  
Influenza Virus ◽  
Respiratory Syncytial Virus ◽  
Rural Areas ◽  
Influenza A ◽  
Respiratory Infections ◽  
Influenza B Virus ◽  
Influenza B ◽  
Virus Infections ◽  
Syncytial Virus ◽  
One Year

SUMMARYAmong 741 children under 5 years admitted to hospital with respiratory infections during two winters, infection with influenza A virus was diagnosed in 70 (9%), with influenza B virus in 8 (1%), and with respiratory syncytial virus (RSV) in 259 (35 %). Both influenza virus and RSV infections were diagnosed most frequently in children under the age of one year, and diagnosed more frequently in males than females. Influenza illnesses were more severe in boys than girls. Both infections occurred more often, but were not more severe, in children from a conurbation than in those from ‘rural’ areas. Convulsions were the cause of 36% of admissions with influenza A infections, but were rare in RSV infections. Bronchiolitis was the reason for 39% of admissions with RSV infections, but was rare in influenza infections. It is suggested that infants admitted to hospital are a good source of influenza virus strains for monitoring arttigenic variation.

scholarly journals Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay

2003 ◽  
Vol 109 (1) ◽  
pp. 39-45 ◽  
Author(s):  
M Gueudin ◽  
A Vabret ◽  
J Petitjean ◽  
S Gouarin ◽  
J Brouard ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Virus Rna ◽  
Syncytial Virus

Evaluation of the incidence of identified types of respiratory viruses in a selected population of inhabitants of the kuyavian-pomeranian voivodeship

2018 ◽  
Vol 54 (3) ◽  
pp. 151-158
Author(s):  
Małgorzata Smoguła ◽  
Marta Pawłowska ◽  
Celestyna Mila-Kierzenkowska ◽  
Joanna Malinowska ◽  
Jerzy Kasprzak ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Real Time ◽  
Genetic Material ◽  
Respiratory Viruses ◽  
Influenza B Virus ◽  
Influenza B ◽  
Rt Pcr ◽  
Syncytial Virus ◽  
Tract Infections ◽  
Epidemiological Situation

Introduction: Respiratory tract infections are caused by various factors including respiratory viruses, for example influenza virus and respiratory syncytial virus (RSV). Surveillance of influenza and other respiratory viruses is carried out as part of the SENTINEL and NON SENTINEL programs in Poland.<br>Aim of study: Determination of the frequency of selected types of influenza and RSV viruses using real-time reverse transcription polymerase chain reaction method (real-time RT-PCR) in a selected population of inhabitants of the kuyavian-pomeranian voivodeship in the epidemic season 2017/2018, i.e. from 1 October 2017 to 30 April 2018.<br>Material and methods: 201 throat and nasal swabs were tested using real-time RT-PCR method for the detection of the presence of genetic material RNA of influenza virus and RSV. The study population was divided into 7 groups depending on age.<br>Results. Positive samples accounted for 48.26% of the swabs tested. The genetic material RNA of influenza virus was found in 95 (47.26) samples and RSV in 2 (1,00%) samples. Influenza B virus was most frequently isolated. The most prevalence of infections was observed in people under 25 years of age.<br>Conclusions: Surveillance of influenza in the SENTINEL and NON SENTINEL programs constitute an important role in assessing the virological and epidemiological situation prevailing in a given area. The results cover part of the population, hence the data may be underestimated. It is necessary to continue studies and observe the virological and epidemiological situation in the polish population.

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