scholarly journals Evaluation of protection against Chlamydophila abortus challenge after DNA immunization with the major outer-membrane protein-encoding gene in pregnant and non-pregnant mice

2003 ◽  
Vol 52 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Céline Héchard ◽  
Olivier Grépinet ◽  
Annie Rodolakis
Gene ◽  
1995 ◽  
Vol 158 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Marcos Fernández-Mora ◽  
Ricardo Oropeza ◽  
JoséLuis Puente ◽  
Edmundo Calva

2005 ◽  
Vol 12 (6) ◽  
pp. 770-777 ◽  
Author(s):  
Morag Livingstone ◽  
Gary Entrican ◽  
Sean Wattegedera ◽  
David Buxton ◽  
Iain J. McKendrick ◽  
...  

ABSTRACT Chlamydophila abortus is one of the major causes of infectious abortion in pregnant sheep (enzootic abortion of ewes or EAE) worldwide. Organisms shed in infected placentas and uterine discharges at lambing time are the main sources of environmental contamination, responsible for transmission to susceptible animals and possible human contacts. In the present study, a recently developed test, based on a recombinant fragment of the polymorphic outer membrane protein POMP90 (rOMP90-4 indirect enzyme-linked immunosorbent assay [iELISA]) and one based on the variable segment 2 (VS2) region of the major outer membrane protein (MOMP) (MOMP VS2 iELISA) were compared using sera from C. abortus-infected ewes at different stages throughout pregnancy. The rOMP90 iELISA detected antibody much earlier in pregnancy than the MOMP iELISA, which, like the complement fixation test, detected antibody only at the time of abortion or lambing. No anti-MOMP antibody response could be detected in three of seven experimentally infected ewes. Furthermore, the rOMP90 iELISA detected antibody in an animal that seroconverted during the course of the study, which the MOMP iELISA failed to detect. Overall, the results show that the rOMP90-4 iELISA is considerably more sensitive than the MOMP VS2 iELISA for identifying animals infected with C. abortus. Earlier detection of infection will allow appropriate control measures to be taken to reduce environmental contamination, thus limiting the spread of infection, financial losses, and the possible risks of zoonotic transmission to humans.


Sign in / Sign up

Export Citation Format

Share Document