scholarly journals Manuka honey inhibits the development of Streptococcus pyogenes biofilms and causes reduced expression of two fibronectin binding proteins

Microbiology ◽  
2012 ◽  
Vol 158 (3) ◽  
pp. 781-790 ◽  
Author(s):  
Sarah E. Maddocks ◽  
Marta Salinas Lopez ◽  
Richard S. Rowlands ◽  
Rose A. Cooper
Keyword(s):  
Streptococcus Pyogenes ◽  
Binding Proteins ◽  
Manuka Honey ◽  
Fibronectin Binding

scholarly journals Protein F1 and Streptococcus pyogenes Resistance to Phagocytosis

2007 ◽  
Vol 75 (6) ◽  
pp. 3188-3191 ◽  
Author(s):  
Kendra A. Hyland ◽  
Beinan Wang ◽  
P. Patrick Cleary
Keyword(s):  
Epithelial Cells ◽  
Protein Expression ◽  
Streptococcus Pyogenes ◽  
Binding Proteins ◽  
M Protein ◽  
Partial Inhibition ◽  
Fibronectin Binding ◽  
Negative Mutant

ABSTRACT Streptococcus pyogenes is a major cause of pharyngitis in humans and encodes several fibronectin-binding proteins. M protein and protein F1 (PrtF1/SfbI) are differentially regulated by CO2 and O2, respectively, and both mediate the invasion of epithelial cells. This study examined whether PrtF1/SfbI shares other properties with M protein. Expression of the PrtF1/SfbI protein by an M-negative mutant conferred resistance to phagocytosis and partial inhibition of C3 deposition on the S. pyogenes surface.

scholarly journals Function of the Fibronectin-Binding Serum Opacity Factor of Streptococcus pyogenes in Adherence to Epithelial Cells

2004 ◽  
Vol 72 (7) ◽  
pp. 4302-4308 ◽  
Author(s):  
Sonja Oehmcke ◽  
Andreas Podbielski ◽  
Bernd Kreikemeyer
Keyword(s):  
Epithelial Cells ◽  
Streptococcus Pyogenes ◽  
Binding Proteins ◽  
Dissociation Constants ◽  
Cell Adherence ◽  
Latex Beads ◽  
Serum Opacity Factor ◽  
Order Of Magnitude ◽  
Fibronectin Binding ◽  
Adherence Inhibition

ABSTRACT The serum opacity factor (SOF) of Streptococcus pyogenes is a serotyping tool and pathogenesis factor. Using SOF-coated latex beads in cell adherence assays and antiserum directed against SOF in S. pyogenes-HEp-2 cell adherence inhibition experiments, we demonstrate SOF involvement in the fibronectin-mediated adherence of S. pyogenes to epithelial cells. SOF exclusively targets the 30-kDa N-terminal region of fibronectin. The interaction revealed association and dissociation constants 1 order of magnitude lower than those of other S. pyogenes fibronectin-binding proteins.

scholarly journals Distribution and Antigenicity of Fibronectin Binding Proteins (SfbI and SfbII) of Streptococcus pyogenes Clinical Isolates from the Northern Territory, Australia

2000 ◽  
Vol 38 (1) ◽  
pp. 389-392
Author(s):  
Alison M. Goodfellow ◽  
Megan Hibble ◽  
Susanne R. Talay ◽  
Bernd Kreikemeyer ◽  
Bart J. Currie ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Immunoglobulin G ◽  
Binding Proteins ◽  
Northern Territory ◽  
Clinical Isolates ◽  
Group A Streptococci ◽  
Streptococcal Infections ◽  
Group A ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

ABSTRACT Fibronectin binding proteins play an important role in the adherence and invasion of group A streptococci (GAS). Genotypically distinct GAS isolates were screened for the presence and expression of two streptococcal fibronectin binding protein genes, sfbI and sfbII . Of the tested strains, 64 and 36% were shown to harbor and express the sfbI and sfbII genes, respectively. All sfbII -positive strains tested were also positive for sfbI , but only 28% of the sfbII -negative strains were positive for sfbI . High levels of immunoglobulin G antibodies to both SfbI and SfbII were found in sera from 80 subjects with defined streptococcal infections.

scholarly journals Association between Resistance to Erythromycin and the Presence of the Fibronectin Binding Protein F1 Gene, prtF1, in Streptococcus pyogenes Isolates from German Pediatric Patients

2005 ◽  
Vol 49 (7) ◽  
pp. 2990-2993 ◽  
Author(s):  
Maria Haller ◽  
Kirsten Fluegge ◽  
Sandra Jasminder Arri ◽  
Brit Adams ◽  
Reinhard Berner
Keyword(s):  
Streptococcus Pyogenes ◽  
Pediatric Patients ◽  
Group A Streptococcus ◽  
Binding Protein ◽  
Macrolide Resistance ◽  
Group A ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding ◽  
Cell Invasiveness

ABSTRACT A total of 301 German pediatric group A streptococcus isolates were screened for the presence of macrolide resistance and the fibronectin binding protein F1 gene (prtF1) encoding an adhesin and cell invasiveness protein. The prtF1 gene was present significantly more often among macrolide-resistant isolates. The majority of these were not clonally related.

scholarly journals Fibronectin‐Binding Proteins and Fibrinogen‐Binding Clumping Factors Play Distinct Roles in Staphylococcal Arthritis and Systemic Inflammation

2005 ◽  
Vol 191 (5) ◽  
pp. 791-798 ◽  
Author(s):  
Niklas Palmqvist ◽  
Timothy Foster ◽  
J. Ross Fitzgerald ◽  
Elisabet Josefsson ◽  
Andrzej Tarkowski
Keyword(s):  
Systemic Inflammation ◽  
Binding Proteins ◽  
Fibrinogen Binding ◽  
Fibronectin Binding

scholarly journals Dissociation Rate Constants of Human Fibronectin Binding to Fibronectin-binding Proteins on LivingStaphylococcus aureusIsolated from Clinical Patients

2012 ◽  
Vol 287 (9) ◽  
pp. 6693-6701 ◽  
Author(s):  
Nadia N. Casillas-Ituarte ◽  
Brian H. Lower ◽  
Supaporn Lamlertthon ◽  
Vance G. Fowler ◽  
Steven K. Lower
Keyword(s):  
Rate Constants ◽  
Binding Proteins ◽  
Dissociation Rate ◽  
Human Fibronectin ◽  
Fibronectin Binding ◽  
Dissociation Rate Constants
Sign in / Sign up

Export Citation Format

Share Document