Influence of the ADP/ATP ratio, 2-oxoglutarate and divalent ions on Azospirillum brasilense PII protein signalling

Microbiology ◽  
2012 ◽  
Vol 158 (6) ◽  
pp. 1656-1663 ◽  
Author(s):  
Edileusa C. M. Gerhardt ◽  
Luíza M. Araújo ◽  
Ronny R. Ribeiro ◽  
Leda S. Chubatsu ◽  
Marcelo Scarduelli ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Divalent Ions ◽  
Pii Protein

scholarly journals The Protein-Protein Interaction Network Reveals a Novel Role of the Signal Transduction Protein PII in the Control of c-di-GMP Homeostasis in Azospirillum brasilense

mSystems ◽  
2020 ◽  
Vol 5 (6) ◽  
Author(s):  
Edileusa C. M. Gerhardt ◽  
Erick Parize ◽  
Fernanda Gravina ◽  
Flávia L. D. Pontes ◽  
Adrian R. S. Santos ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Protein Interaction Network ◽  
Azospirillum Brasilense ◽  
Interaction Network ◽  
Metabolome Analysis ◽  
Content Type ◽  
Pii Protein ◽  
Protein Protein Interaction ◽  
Plant Growth Promoting ◽  
Pii Proteins

ABSTRACT The PII family comprises a group of widely distributed signal transduction proteins ubiquitous in prokaryotes and in the chloroplasts of plants. PII proteins sense the levels of key metabolites ATP, ADP, and 2-oxoglutarate, which affect the PII protein structure and thereby the ability of PII to interact with a range of target proteins. Here, we performed multiple ligand fishing assays with the PII protein orthologue GlnZ from the plant growth-promoting nitrogen-fixing bacterium Azospirillum brasilense to identify 37 proteins that are likely to be part of the PII protein-protein interaction network. Among the PII targets identified were enzymes related to nitrogen and fatty acid metabolism, signaling, coenzyme synthesis, RNA catabolism, and transcription. Direct binary PII-target complex was confirmed for 15 protein complexes using pulldown assays with recombinant proteins. Untargeted metabolome analysis showed that PII is required for proper homeostasis of important metabolites. Two enzymes involved in c-di-GMP metabolism were among the identified PII targets. A PII-deficient strain showed reduced c-di-GMP levels and altered aerotaxis and flocculation behavior. These data support that PII acts as a major metabolic hub controlling important enzymes and the homeostasis of key metabolites such as c-di-GMP in response to the prevailing nutritional status. IMPORTANCE The PII proteins sense and integrate important metabolic signals which reflect the cellular nutrition and energy status. Such extraordinary ability was capitalized by nature in such a way that the various PII proteins regulate different facets of metabolism by controlling the activity of a range of target proteins by protein-protein interactions. Here, we determined the PII protein interaction network in the plant growth-promoting nitrogen-fixing bacterium Azospirillum brasilense. The interactome data along with metabolome analysis suggest that PII functions as a master metabolic regulator hub. We provide evidence that PII proteins act to regulate c-di-GMP levels in vivo and cell motility and adherence behaviors.

Dopant aggregation and precipitation in alkali halides doped with divalent ions

1980 ◽  
Vol 41 (C6) ◽  
pp. C6-68-C6-71 ◽  
Author(s):  
J. Corish ◽  
J. M. Quigley ◽  
C. R. A. Catlow ◽  
P. W.M. Jacobs
Keyword(s):  
Alkali Halides ◽  
Divalent Ions

Снижение эффективности формирования биопленок и их устойчивости к окислительному стрессу и высушиванию у ahpC мутанта Azospirillum Brasilense

2021 ◽  
Vol 90 (1) ◽  
pp. 64-75
Author(s):  
А. В. Шелудько ◽  
Д. И. Мокеев ◽  
С. С. Евстигнеева ◽  
Ю. А. Филипьечева ◽  
А. М. Буров ◽  
...  
Keyword(s):  
Azospirillum Brasilense

scholarly journals SOYBEAN CULTIVATION IN A CROP-LIVESTOCK SYSTEM WITH Azospirillum brasilense INOCULATION

2018 ◽  
Vol 31 (1) ◽  
pp. 1-8
Author(s):  
MARCOS DA SILVA BRUM ◽  
THOMAS NEWTON MARTIN ◽  
VINÍCIUS SANTOS DA CUNHA ◽  
LUIZ FERNANDO TELEKEN GRANDO ◽  
ALEX TAGLIAPIETRA SCHONELL
Keyword(s):  
Azospirillum Brasilense ◽  
Block Design ◽  
Experimental Period ◽  
Height Control ◽  
Grain Productivity ◽  
Randomized Complete Block Design ◽  
Grazing Systems ◽  
Soybean Plants ◽  
Santa Maria ◽  
Livestock System

ABSTRACT The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.

scholarly journals Split NanoLuc technology allows quantitation of interactions between PII protein and its receptors with unprecedented sensitivity and reveals transient interactions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rokhsareh Rozbeh ◽  
Karl Forchhammer
Keyword(s):  
The Novel ◽  
Multiple Target ◽  
Interacting Protein ◽  
Effector Molecule ◽  
Target Proteins ◽  
Pii Protein ◽  
Protein X ◽  
Cellular Context ◽  
Pii Proteins ◽  
Transient Interactions

AbstractPII proteins constitute a widespread signal transduction superfamily in the prokaryotic world. The canonical PII signal proteins sense metabolic state of the cells by binding the metabolite molecules ATP, ADP and 2-oxoglutarate. Depending on bound effector molecule, PII proteins interact with and modulate the activity of multiple target proteins. To investigate the complexity of interactions of PII with target proteins, analytical methods that do not disrupt the native cellular context are required. To this purpose, split luciferase proteins have been used to develop a novel complementation reporter called NanoLuc Binary Technology (NanoBiT). The luciferase NanoLuc is divided in two subunits: a 18 kDa polypeptide termed “Large BiT” and a 1.3 kDa peptide termed “Small BiT”, which only weakly associate. When fused to proteins of interest, they reconstitute an active luciferase when the proteins of interest interact. Therefore, we set out to develop a new NanoBiT sensor based on the interaction of PII protein from Synechocystis sp. PCC6803 with PII-interacting protein X (PipX) and N-acetyl-L-glutamate kinase (NAGK). The novel NanoBiT sensor showed unprecedented sensitivity, which made it possible to detect even weak and transient interactions between PII variants and their interacting partners, thereby shedding new light in PII signalling processes.

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