Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule
2009 ◽
Vol 90
(7)
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pp. 1730-1733
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The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.
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2004 ◽
Vol 149
(11)
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pp. 2271-2276
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1998 ◽
Vol 72
(11)
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pp. 9404-9406
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