Construction of gene transfer vectors based on simian adenovirus 7

The complete nucleotide sequence of an isolate of simian adenovirus 7 (SAdV-7) was determined. The genome organization of this isolate was found to be similar to that of other primate adenoviruses with two principal notable points: severe truncation of the E1A and E1B 19K proteins and an E3 region encoding only the 12.5K homologue. The viral gene products of SAdV-7 are most closely related to simian adenovirus 1 (SAdV-1), and like SAdV-1, are related to the human adenovirus species HAdV-F, such as the enteric adenoviruses HAdV-40 and HAdV-41 and the recently defined HAdV-G (HAdV-52). Two kinds of gene transfer vectors were made: a replication-competent SAdV-7-based vector with no genomic deletion, and a standard replication-incompetent vector deleted for E1. Importantly, the E1-deleted vector could be propagated to high titre by trans-complementation in human HEK 293 cells.

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Valproic acid enhances gene expression from viral gene transfer vectors

Journal of Virological Methods ◽

10.1016/j.jviromet.2004.11.023 ◽

2005 ◽

Vol 125 (1) ◽

pp. 23-33 ◽

Cited By ~ 25

Author(s):

Shongshan Fan ◽

Casey A. Maguire ◽

Servio H. Ramirez ◽

Birgit Bradel-Tretheway ◽

Ramil Sapinoro ◽

...

Keyword(s):

Gene Expression ◽

Valproic Acid ◽

Gene Transfer ◽

Viral Gene ◽

Viral Gene Transfer ◽

Transfer Vectors

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661. Development of Non-Human Adenovirus Based Gene Transfer Vectors

Molecular Therapy ◽

10.1016/s1525-0016(16)41103-2 ◽

2003 ◽

Vol 7 (5) ◽

pp. S257

Keyword(s):

Gene Transfer ◽

Human Adenovirus ◽

Transfer Vectors

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Antimicrobial activity of antiproteinases

Biochemical Society Transactions ◽

10.1042/bst0300111 ◽

2002 ◽

Vol 30 (2) ◽

pp. 111-115 ◽

Cited By ~ 81

Author(s):

J.-M. Sallenave

Keyword(s):

Gene Transfer ◽

Molecular Mass ◽

Neutrophil Elastase ◽

Antimicrobial Properties ◽

Specific Inhibitor ◽

Antimicrobial Activities ◽

Viral Gene ◽

Transfer Vectors

Low-molecular-mass neutrophil elastase inhibitors have been shown to be important in the control of lung inflammation. In addition to inhibiting the enzyme neutrophil elastase, these low-molecular-mass compounds (10 kDa) have been shown to have other activities. For example, secretory leucocyte proteinase inhibitor (SLPI) and elastase-specific inhibitor/SKALP (skin-derived antileucoproteinase)/elafin have also been shown to have ‘defensin’-like antimicrobial activities. Indeed, these inhibitors have antimicrobial properties in vitro against bacteria, fungi and, potentially, HIV. In addition, we have shown, using an adenovirus-mediated gene transfer overexpression strategy, that elafin is also active against Pseudomonas aeruginosa infection in mice in vivo. The mechanism of action is currently under investigation. In addition to these direct or indirect effects on microbes, it has been shown that lipopolysaccharide is able to up-regulate SPLI production in macrophages in vitro, and that the addition of recombinant SLPI to human monocytes or the transfection of macrophages with SPLI can down-regulate pro-inflammatory mediators such as tumour necrosis factor, presumably to limit self-damaging excessive inflammation. Using viral gene transfer vectors, we are currently investigating the potential of these inhibitors in various models of inflammation in vivo.

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Regulation of the pyruvate metabolism node by monogene and polygene engineering of HEK-293 cells

RSC Advances ◽

10.1039/c9ra07418j ◽

2019 ◽

Vol 9 (61) ◽

pp. 35760-35770

Author(s):

Li Xie ◽

Junqing Miao ◽

Xiangchao Li ◽

Xiaoping Yi ◽

Ju Chu

Keyword(s):

Human Adenovirus ◽

Pyruvate Metabolism ◽

Hek 293 ◽

Hek 293 Cells ◽

293 Cells

HEK-293 cells are increasingly being used in the production of human adenovirus (HAdV) vaccines.

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Infection kinetics of human adenovirus serotype 41 in HEK 293 cells

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762009000500013 ◽

2009 ◽

Vol 104 (5) ◽

pp. 736-744 ◽

Cited By ~ 11

Author(s):

Joselma Siqueira-Silva ◽

Fernanda Perez Yeda ◽

Anne-Laure Favier ◽

Paulette Mezin ◽

Misael Leonardo Silva ◽

...

Keyword(s):

Human Adenovirus ◽

Adenovirus Serotype ◽

Hek 293 ◽

Hek 293 Cells ◽

293 Cells ◽

Kinetics Of

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Spacers Increase the Accessibility of Peptide Ligands Linked to the Carboxyl Terminus of Adenovirus Minor Capsid Protein IX

Journal of Virology ◽

10.1128/jvi.78.7.3470-3479.2004 ◽

2004 ◽

Vol 78 (7) ◽

pp. 3470-3479 ◽

Cited By ~ 69

Author(s):

Jort Vellinga ◽

Martijn J. W. E. Rabelink ◽

Steve J. Cramer ◽

Diana J. M. van den Wollenberg ◽

Hans Van der Meulen ◽

...

Keyword(s):

Gene Transfer ◽

Capsid Protein ◽

Human Adenovirus ◽

Peptide Ligands ◽

Electron Microscopic ◽

Spacer Length ◽

Minor Capsid Protein ◽

Transfer Vectors ◽

Group B ◽

Protein Ix

ABSTRACT The efficiency and specificity of gene transfer with human adenovirus (hAd)-derived gene transfer vectors would be improved if the native viral tropism could be modified. Here, we demonstrate that the minor capsid protein IX (pIX), which is present in 240 copies in the Ad capsid, can be exploited as an anchor for heterologous polypeptides. Protein IX-deleted hAd5 vectors were propagated in hAd5 helper cells expressing pIX variants, with heterologous carboxyl-terminal extensions of up to 113 amino acids in length. The extensions evaluated consist of alpha-helical spacers up to 75 Å in length and to which peptide ligands were fused. The pIX variants were efficiently incorporated into the capsids of Ad particles. On intact particles, the MYC-tagged-pIX molecules were readily accessible to anti-MYC antibodies, as demonstrated by electron microscopic analyses of immunogold-labeled virus particles. The labeling efficiency improved with increasing spacer length, suggesting that the spacers lift and expose the ligand at the capsid surface. Furthermore, we found that the addition of an integrin-binding RGD motif to the pIX markedly stimulated the transduction of coxsackievirus group B and hAd receptor-deficient endothelioma cells, demonstrating the utility of pIX modification in gene transfer. Our data demonstrate that the minor capsid protein IX can be used as an anchor for the addition of polypeptide ligands to Ad particles.

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