scholarly journals White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection

2006 ◽  
Vol 87 (7) ◽  
pp. 1903-1908 ◽  
Author(s):  
Xixian Xie ◽  
Feng Yang
Keyword(s):  
Virus Infection ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Early Stage ◽  
White Spot ◽  
Structural Protein ◽  
Virion Envelope ◽  
White Spot Syndrome ◽  
Triton X

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction also indicated that VP24 behaves as an envelope protein. Immunoelectron microscopy further confirmed that VP24 is located in the virion envelope. Far-Western experiments showed that VP24 interacts with VP28, another major envelope protein of the WSSV virion. To investigate the function of VP24, WSSV was neutralized with various amounts of anti-VP24 IgG and injected into crayfish. The results showed that anti-VP24 IgG could partially attenuate infection with WSSV. It is concluded that VP24 is an envelope protein and functions at an early stage in virus infection.

scholarly journals A novel envelope protein involved in White spot syndrome virus infection

2005 ◽  
Vol 86 (5) ◽  
pp. 1357-1361 ◽  
Author(s):  
Ru Huang ◽  
Yunli Xie ◽  
Jianhong Zhang ◽  
Zhengli Shi
Keyword(s):  
Escherichia Coli ◽  
Virus Infection ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Open Reading Frame ◽  
White Spot ◽  
Structural Protein ◽  
Reading Frame ◽  
Intact Virion ◽  
White Spot Syndrome

One open reading frame (designated vp76) from the White spot syndrome virus (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, vp76 was expressed in Escherichia coli and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.

scholarly journals Envelope Protein Gene VP466-a Target for PCR Detection of White Spot Syndrome Virus in Shrimp

2016 ◽  
pp. 65-68 ◽  
Author(s):  
Anwar Hossain ◽  
Santonu Kumar Sanyal ◽  
Mohammad Anwar Siddique ◽  
Raj Kumar Biswas ◽  
Munawar Sultana ◽  
...  
Keyword(s):  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Protein Gene ◽  
Polymerase Chain Reaction Method ◽  
Pcr Detection ◽  
White Spot ◽  
Structural Protein ◽  
Gene Encoding ◽  
White Spot Disease ◽  
White Spot Syndrome

White spot syndrome virus (WSSV) is an enveloped and double-stranded DNA virus that belongs to the family Nimaviridae and genus Whispovirus, causing white spot disease (WSD) in shrimp. The virus is highly virulent and leads to 100% mortality within 10 days. Detection of WSSV and segregation of infected brood shrimp, post-larvae and cultured shrimp are currently considered as containment strategies to reduce the spread of WSD. This investigation describes a polymerase chain reaction method to detect WSSV in WSD infected cultured shrimp targeting VP466 gene encoding the large structural protein in virus particle. In silico homology analysis of the primer pair designed in this work clearly identified WSSV VP466 gene sequence with 100% specificity. A total of 16 shrimp samples from 16 farms were selected, where 6 shrimp samples were with characteristics WSD spot and 10 shrimp samples were asymptomatic. Among the 16 shrimp samples, 12 showed PCR positive amplifications for major envelope protein gene VP466. Sequencing of the amplicons followed by homology searching using BLAST further confirmed the presence of WSSV. Phylogenetic analysis of VP466 gene sequences showed its close proximity to the WSSV strain of Indian origin. The present study demonstrates that the envelope protein VP466 gene as a specific target for PCR detection and characterization of WSSV in WSD infected and carrier shrimpsBangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 65-68

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