scholarly journals A novel envelope protein involved in White spot syndrome virus infection

2005 ◽  
Vol 86 (5) ◽  
pp. 1357-1361 ◽  
Author(s):  
Ru Huang ◽  
Yunli Xie ◽  
Jianhong Zhang ◽  
Zhengli Shi
Keyword(s):  
Escherichia Coli ◽  
Virus Infection ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Open Reading Frame ◽  
White Spot ◽  
Structural Protein ◽  
Reading Frame ◽  
Intact Virion ◽  
White Spot Syndrome

One open reading frame (designated vp76) from the White spot syndrome virus (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, vp76 was expressed in Escherichia coli and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.

scholarly journals Characterization of an envelope protein (VP110) of White spot syndrome virus

2006 ◽  
Vol 87 (7) ◽  
pp. 1909-1915 ◽  
Author(s):  
Li Li ◽  
Shumei Lin ◽  
Feng Yang
Keyword(s):  
Mass Spectrometry ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Open Reading Frame ◽  
Host Cells ◽  
White Spot ◽  
Genome Database ◽  
Reading Frame ◽  
Rgd Motif ◽  
White Spot Syndrome

A protein of 110 kDa (termed VP110) from the envelope fraction of White spot syndrome virus (WSSV) was identified by SDS-PAGE and mass spectrometry. The resulting amino acid sequence matched an open reading frame (wsv035) containing an Arg–Gly–Asp (RGD) motif in the WSSV genome database. To validate the mass-spectrometry result, the C-terminal segment of the wsv035 open reading frame was expressed in Escherichia coli as a fusion protein, which was used to produce specific antibody. Analysis by Western blotting and immunoelectron microscopy demonstrated that VP110 was an envelope protein of WSSV. An interaction analysis was performed between VP110 and the host cells, using a fluorescence assay and a competitive-inhibition assay. The results showed that VP110 was capable of attaching to host cells and that adhesion could be inhibited by synthetic RGDT peptides, suggesting that the RGD motif in the VP110 sequence may play a role in WSSV infection.

scholarly journals White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection

2006 ◽  
Vol 87 (7) ◽  
pp. 1903-1908 ◽  
Author(s):  
Xixian Xie ◽  
Feng Yang
Keyword(s):  
Virus Infection ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Early Stage ◽  
White Spot ◽  
Structural Protein ◽  
Virion Envelope ◽  
White Spot Syndrome ◽  
Triton X

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction also indicated that VP24 behaves as an envelope protein. Immunoelectron microscopy further confirmed that VP24 is located in the virion envelope. Far-Western experiments showed that VP24 interacts with VP28, another major envelope protein of the WSSV virion. To investigate the function of VP24, WSSV was neutralized with various amounts of anti-VP24 IgG and injected into crayfish. The results showed that anti-VP24 IgG could partially attenuate infection with WSSV. It is concluded that VP24 is an envelope protein and functions at an early stage in virus infection.

Immunogenicity and protective efficacy of a major White Spot Syndrome Virus (WSSV) envelope protein VP24 expressed in Escherichia coli against WSSV

2014 ◽  
Vol 123 ◽  
pp. 17-24 ◽  
Author(s):  
Ancy Thomas ◽  
Naduvilamuriparampu Saidumuhammed Sudheer ◽  
Karthik Viswanathan ◽  
Viswanath Kiron ◽  
Issac S. Bright Singh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Protective Efficacy ◽  
White Spot ◽  
White Spot Syndrome

scholarly journals Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus

2002 ◽  
Vol 83 (2) ◽  
pp. 471-477 ◽  
Author(s):  
Xiaobo Zhang ◽  
Canhua Huang ◽  
Xun Xu ◽  
Choy L. Hew
Keyword(s):  
Fusion Protein ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Protein Gene ◽  
Temporal Analysis ◽  
White Spot ◽  
Outer Envelope ◽  
Reading Frame ◽  
Transmission Electron ◽  
White Spot Syndrome

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. In the present study, an open reading frame (termed the p22 gene) was revealed from a WSSV cDNA library. The gene was expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli and purified. Specific antibody was raised using the purified fusion protein (GST–P22). Temporal analysis showed that the p22 gene was a late gene. After binding between purified WSSV virions and anti-GST–P22 IgG followed by labelling with gold-labelled secondary antibody, the gold particles, under a transmission electron microscope, could be found along the outer envelope of WSSV virions. This experiment suggests that the p22 gene encodes an envelope protein of the virus.

scholarly journals Envelope Protein Gene VP466-a Target for PCR Detection of White Spot Syndrome Virus in Shrimp

2016 ◽  
pp. 65-68 ◽  
Author(s):  
Anwar Hossain ◽  
Santonu Kumar Sanyal ◽  
Mohammad Anwar Siddique ◽  
Raj Kumar Biswas ◽  
Munawar Sultana ◽  
...  
Keyword(s):  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Protein Gene ◽  
Polymerase Chain Reaction Method ◽  
Pcr Detection ◽  
White Spot ◽  
Structural Protein ◽  
Gene Encoding ◽  
White Spot Disease ◽  
White Spot Syndrome

White spot syndrome virus (WSSV) is an enveloped and double-stranded DNA virus that belongs to the family Nimaviridae and genus Whispovirus, causing white spot disease (WSD) in shrimp. The virus is highly virulent and leads to 100% mortality within 10 days. Detection of WSSV and segregation of infected brood shrimp, post-larvae and cultured shrimp are currently considered as containment strategies to reduce the spread of WSD. This investigation describes a polymerase chain reaction method to detect WSSV in WSD infected cultured shrimp targeting VP466 gene encoding the large structural protein in virus particle. In silico homology analysis of the primer pair designed in this work clearly identified WSSV VP466 gene sequence with 100% specificity. A total of 16 shrimp samples from 16 farms were selected, where 6 shrimp samples were with characteristics WSD spot and 10 shrimp samples were asymptomatic. Among the 16 shrimp samples, 12 showed PCR positive amplifications for major envelope protein gene VP466. Sequencing of the amplicons followed by homology searching using BLAST further confirmed the presence of WSSV. Phylogenetic analysis of VP466 gene sequences showed its close proximity to the WSSV strain of Indian origin. The present study demonstrates that the envelope protein VP466 gene as a specific target for PCR detection and characterization of WSSV in WSD infected and carrier shrimpsBangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 65-68

scholarly journals The Unique Stacked Rings in the Nucleocapsid of the White Spot Syndrome Virus Virion Are Formed by the Major Structural Protein VP664, the Largest Viral Structural Protein Ever Found

2005 ◽  
Vol 79 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Jiann-Horng Leu ◽  
Jyh-Ming Tsai ◽  
Han-Ching Wang ◽  
Andrew H.-J. Wang ◽  
Chung-Hsiung Wang ◽  
...  
Keyword(s):  
Immunoelectron Microscopy ◽  
Time Course ◽  
White Spot Syndrome Virus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
White Spot ◽  
Sequencing Analysis ◽  
Structural Protein ◽  
Reading Frame ◽  
White Spot Syndrome

ABSTRACT One unique feature of the shrimp white spot syndrome virus (WSSV) genome is the presence of a giant open reading frame (ORF) of 18,234 nucleotides that encodes a long polypeptide of 6,077 amino acids with a hitherto unknown function. In the present study, by applying proteomic methodology to analyze the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile of purified WSSV virions by liquid chromatography-mass spectrometry (LC-MS/MS), we found that this giant polypeptide, designated VP664, is one of the viral structural proteins. The existence of the corresponding 18-kb transcript was confirmed by sequencing analysis of reverse transcription-PCR products, which also showed that vp664 was intron-less. A time course analysis showed that this transcript was actively transcribed at the late stage, suggesting that this gene product should contribute primarily to the assembly and morphogenesis of the virion. Several polyclonal antisera against this giant protein were prepared, and one of them was successfully used for immunoelectron microscopy analysis to localize the protein in the virion. Immunoelectron microscopy with a gold-labeled secondary antibody showed that the gold particles were regularly distributed around the periphery of the nucleocapsid with a periodicity that matched the characteristic stacked ring subunits that appear as striations. From this and other evidence, we argue that this giant ORF in fact encodes the major WSSV nucleocapsid protein.

Sign in / Sign up

Export Citation Format

Share Document