scholarly journals Thermostable variants are not generally represented in foot-and-mouth disease virus quasispecies

2007 ◽  
Vol 88 (3) ◽  
pp. 859-864 ◽  
Author(s):  
Roberto Mateo ◽  
Eva Luna ◽  
Mauricio G. Mateu
Keyword(s):  
Disease Virus ◽  
General Feature ◽  
Rna Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Persistent Infections ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Negative Effect ◽  
Virus Genomes

A severe limitation to fully realize the dramatic potential for adaptation of RNA virus quasispecies may occur if mutations in vast regions of the sequence space of virus genomes lead to significant decreases in biological fitness. In this study the detection and selection by heat of thermostable variants from different foot-and-mouth disease virus (FMDV) populations were attempted, in order to explore whether FMDV may generally accept a substantial increase in thermostability without compromising its infectivity. The results obtained with both uncloned and cloned populations of different serotypes, recovered from cytolytic or persistent infections and subjected to either very few passages or extensive passaging in cells, indicate that the presence of thermostable virus variants, even in small proportions, is not a general feature of FMDV quasispecies. This suggests that no substantial increase in the thermostability of FMDV may readily occur without a negative effect on viral function.

Comparative sequence analysis of representative foot-and-mouth disease virus genomes from Southeast Asia

Virus Genes ◽  
2011 ◽  
Vol 43 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Nor Faizah Abdul-Hamid ◽  
Müge Fırat-Saraç ◽  
Alan D. Radford ◽  
Nick J. Knowles ◽  
Donald P. King
Keyword(s):  
Sequence Analysis ◽  
Southeast Asia ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Comparative Sequence Analysis ◽  
Mouth Disease ◽  
Comparative Sequence ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Virus Genomes

scholarly journals Reconstruction of the Transmission History of RNA Virus Outbreaks Using Full Genome Sequences: Foot-and-Mouth Disease Virus in Bulgaria in 2011

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49650 ◽  
Author(s):  
Begoña Valdazo-González ◽  
Lilyana Polihronova ◽  
Tsviatko Alexandrov ◽  
Preben Normann ◽  
Nick J. Knowles ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rna Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Full Genome ◽  
Genome Sequences ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
History Of

scholarly journals An Antiviral Mechanism Investigated with Ribavirin as an RNA Virus Mutagen for Foot-and-mouth Disease Virus

BMB Reports ◽  
2006 ◽  
Vol 39 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Chao-Jiang Gu ◽  
Cong-Yi Zheng ◽  
Qian Zhang ◽  
Li-Li Shi ◽  
Yong Li ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rna Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Antiviral Mechanism

Probe capture enrichment next-generation sequencing of complete foot-and-mouth disease virus genomes in clinical samples

2019 ◽  
Vol 272 ◽  
pp. 113703 ◽  
Author(s):  
Nagendrakumar B. Singanallur ◽  
Danielle E. Anderson ◽  
October M. Sessions ◽  
Uma S. Kamaraj ◽  
Timothy R. Bowden ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Clinical Samples ◽  
Next Generation ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Virus Genomes ◽  
Generation Sequencing

scholarly journals Into the Deep (Sequence) of the Foot-and-Mouth Disease Virus Gene Pool: Bottlenecks and Adaptation during Infection in Naïve and Vaccinated Cattle

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 208 ◽  
Author(s):  
Ian Fish ◽  
Carolina Stenfeldt ◽  
Rachel M. Palinski ◽  
Steven J. Pauszek ◽  
Jonathan Arzt
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Genomic Variation ◽  
Selective Sweeps ◽  
Persistent Infections ◽  
Natural Host Species ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot-and-mouth disease virus (FMDV) infects hosts as a population of closely related viruses referred to as a quasispecies. The behavior of this quasispecies has not been described in detail in natural host species. In this study, virus samples collected from vaccinated and non-vaccinated cattle up to 35 days post-experimental infection with FMDV A24-Cruzeiro were analyzed by deep-sequencing. Vaccination induced significant differences compared to viruses from non-vaccinated cattle in substitution rates, entropy, and evidence for adaptation. Genomic variation detected during early infection reflected the diversity inherited from the source virus (inoculum), whereas by 12 days post infection, dominant viruses were defined by newly acquired mutations. Mutations conferring recognized fitness gain occurred and were associated with selective sweeps. Persistent infections always included multiple FMDV subpopulations, suggesting distinct foci of infection within the nasopharyngeal mucosa. Subclinical infection in vaccinated cattle included very early bottlenecks associated with reduced diversity within virus populations. Viruses from both animal cohorts contained putative antigenic escape mutations. However, these mutations occurred during later stages of infection, at which time transmission is less likely to occur. This study improves upon previously published work by analyzing deep sequences of samples, allowing for detailed characterization of FMDV populations over time within multiple hosts.

scholarly journals Deterministic, Compensatory Mutational Events in the Capsid of Foot-and-Mouth Disease Virus in Response to the Introduction of Mutations Found in Viruses from Persistent Infections

2006 ◽  
Vol 81 (4) ◽  
pp. 1879-1887 ◽  
Author(s):  
Roberto Mateo ◽  
Mauricio G. Mateu
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Phenotypic Traits ◽  
Multiplication Rate ◽  
Plaque Size ◽  
Persistent Infections ◽  
Viral Yield ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACT The evolution of foot-and-mouth disease virus (FMDV) (biological clone C-S8c1) in persistently infected cells led to the emergence of a variant (R100) that displayed increased virulence, reduced stability, and other modified phenotypic traits. Some mutations fixed in the R100 genome involved a cluster of highly conserved residues around the capsid pores that participate in interactions with each other and/or between capsid protomers. We have investigated phenotypic and genotypic changes that occurred when these replacements were introduced into the C-S8c1 capsid. The C3007V and M3014L mutations exerted no effect on plaque size or viral yield during lytic infections, or on virion stability, but led to a reduction in biological fitness; the D3009A mutation caused drastic reductions in plaque size and viability. Remarkably, competition of the C3007V mutant with the nonmutated virus invariably resulted in the fixation of the D3009A mutation in the C3007V capsid. In turn, the presence of the D3009A mutation invariably led to the fixation of the M3014L mutation. In both cases, two individually disadvantageous mutations led, together, to an increase in fitness, as the double mutants outcompeted the nonmutated genotype. The higher fitness of C3007V/D3009A was related to a faster multiplication rate. These observations provide evidence for a chain of linked, compensatory mutational events in a defined region of the FMDV capsid. Furthermore, they indicate that the clustering of unique amino acid replacements in viruses from persistent infections may also occur in cytolytic infections in response to changes caused by previous mutations without an involvement of the new mutations in the adaptation to a different environment.

scholarly journals Shifts in the Selection-Drift Balance Drive the Evolution and Epidemiology of Foot-and-Mouth Disease Virus

2008 ◽  
Vol 83 (2) ◽  
pp. 781-790 ◽  
Author(s):  
Damien C. Tully ◽  
Mario A. Fares
Keyword(s):  
Disease Virus ◽  
Protein Structures ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Adaptive Mutations ◽  
Persistent Infections ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Synonymous Sites

ABSTRACT Foot-and-mouth disease virus (FMDV) is the causative agent of an acute vesicular disease affecting wild and domesticated animals. Despite the economic burden of the disease and all efforts to eradicate it, FMD outbreaks continue to emerge unexpectedly in developed and developing countries. Correlation of the mutational dynamics of the virus with its epidemiology remains unexplored. Analysis of 103 complete genomes representing the seven serotypes shows the important role that selection plays in the genomic evolution of viral isolates for serotypes. We identified selection and relaxed constraints due to genetic drift through analyses of synonymous sites. Finally, we investigated interactions between mutations that showed coevolving patterns and analyzed, based on protein structures, slightly deleterious and compensatory mutational dynamics. Specifically, we demonstrate that structurally exposed capsid proteins present a greater number of adaptive mutations and relaxed selection than nonstructural proteins. Such events have been magnified during the evolution of the southern African virus types (SATs). These shifts in selection-drift balance have generated the great antigenic and genetic diversity observed for SAT serotypes and that are responsible for epizootics on the continent of Africa. The high number of slightly deleterious and compensatory mutations in SAT serotypes in structural proteins is testament to such balance plasticity. The significant accumulation of these coevolving mutations in African serotypes supports their contribution in generating adaptive immune-escaping mutants and in establishing persistent infections. The reverse of this pattern in nonstructural proteins reveals the neutral fixation of mutations in the more widely spread and commonly studied Euro-Asiatic serotypes.

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