Three‐percent sucrose acts as a thermostabilizer for cell‐adapted foot‐and‐mouth disease virus without any negative effect on viral growth

2020 ◽  
Vol 128 (5) ◽  
pp. 1524-1531
Author(s):  
J.‐H. Hwang ◽  
Y. Moon ◽  
G. Lee ◽  
M.‐Y. Kim ◽  
K.‐N. Lee ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Viral Growth ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Negative Effect

scholarly journals Thermostable variants are not generally represented in foot-and-mouth disease virus quasispecies

2007 ◽  
Vol 88 (3) ◽  
pp. 859-864 ◽  
Author(s):  
Roberto Mateo ◽  
Eva Luna ◽  
Mauricio G. Mateu
Keyword(s):  
Disease Virus ◽  
General Feature ◽  
Rna Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Persistent Infections ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Negative Effect ◽  
Virus Genomes

A severe limitation to fully realize the dramatic potential for adaptation of RNA virus quasispecies may occur if mutations in vast regions of the sequence space of virus genomes lead to significant decreases in biological fitness. In this study the detection and selection by heat of thermostable variants from different foot-and-mouth disease virus (FMDV) populations were attempted, in order to explore whether FMDV may generally accept a substantial increase in thermostability without compromising its infectivity. The results obtained with both uncloned and cloned populations of different serotypes, recovered from cytolytic or persistent infections and subjected to either very few passages or extensive passaging in cells, indicate that the presence of thermostable virus variants, even in small proportions, is not a general feature of FMDV quasispecies. This suggests that no substantial increase in the thermostability of FMDV may readily occur without a negative effect on viral function.

High resolution surface structure of foot-and-mouth disease virus

1978 ◽  
Vol 36 (2) ◽  
pp. 376-377
Author(s):  
S. S. Breese ◽  
H. L. Bachrach
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Surface Structure ◽  
Disease Virus ◽  
Potassium Phosphate ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Physical Measurements ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Models for the structure of foot-and-mouth disease virus (FMDV) have been proposed from chemical and physical measurements (Brown, et al., 1970; Talbot and Brown, 1972; Strohmaier and Adam, 1976) and from rotational image-enhancement electron microscopy (Breese, et al., 1965). In this report we examine the surface structure of FMDV particles by high resolution electron microscopy and compare it with that of particles in which the outermost capsid protein VP3 (ca. 30, 000 daltons) has been split into smaller segments, two of which VP3a and VP3b have molecular weights of about 15, 000 daltons (Bachrach, et al., 1975).Highly purified and concentrated type A12, strain 119 FMDV (5 mg/ml) was prepared as previously described (Bachrach, et al., 1964) and stored at 4°C in 0. 2 M KC1-0. 5 M potassium phosphate buffer at pH 7. 5. For electron microscopy, 1. 0 ml samples of purified virus and trypsin-treated virus were dialyzed at 4°C against 0. 2 M NH4OAC at pH 7. 3, deposited onto carbonized formvar-coated copper screens and stained with phosphotungstic acid, pH 7. 3.

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