scholarly journals Analysis of the study of the cerebellar pinceau by Korn and Axelrad

2013 ◽  
Author(s):  
Antonin Blot ◽  
Boris Barbour
Keyword(s):  
Purkinje Cell ◽  
Gap Junctions ◽  
Initial Segment ◽  
Axon Initial Segment ◽  
Cerebellar Purkinje Cell ◽  
Basket Cell ◽  
Mauthner Cell ◽  
Parallel Fibres ◽  
Chemical Synapses ◽  
Stimulation Of

The axon initial segment of each cerebellar Purkinje cell is ensheathed by basket cell axons in a structure called the pinceau, which is largely devoid of chemical synapses and gap junctions. These facts and ultrastructural similarities with the axon cap of the teleost Mauthner cell led to the conjecture that the pinceau mediates ephaptic (via the extracellular field) inhibition. Korn and Axelrad published a study in 1980 in which they reported confirmation of this conjecture. We have analysed their results and show that most are likely to be explained by an artefactual signal arising from the massive stimulation of parallel fibres they employed. We reproduce their experiments and confirm that all of their results are consistent with this artefact. Their data therefore provide no evidence regarding the operation of the pinceau.

scholarly journals Purkinje cell neurotransmission patterns cerebellar basket cells into zonal modules that are defined by distinct pinceau sizes

2020 ◽  
Author(s):  
Joy Zhou ◽  
Amanda M. Brown ◽  
Elizabeth P. Lackey ◽  
Marife Arancillo ◽  
Tao Lin ◽  
...  
Keyword(s):  
Purkinje Cell ◽  
Purkinje Cells ◽  
Initial Segment ◽  
Molecular Diversity ◽  
Basket Cell ◽  
Basket Cells ◽  
Conditional Allele ◽  
Functional Zones ◽  
Cell Somata ◽  
Ramón Y Cajal

AbstractRamón y Cajal proclaimed the neuron doctrine based on circuit features he exemplified using cerebellar basket cell projections. Basket cells form dense inhibitory plexuses that wrap Purkinje cell somata and terminate as pinceaux at the initial segment of axons. Here, we demonstrate that HCN1, Kv1.1, PSD95 and GAD67 unexpectedly mark patterns of basket cell pinceaux that map onto Purkinje cell functional zones. Using cell-specific genetic tracing with an Ascl1CreERT2 mouse conditional allele, we reveal that basket cell zones comprise different sizes of pinceaux. We tested whether Purkinje cells instruct the assembly of inhibitory projections into zones, as they do for excitatory afferents. Genetically silencing Purkinje cell neurotransmission blocks the formation of sharp Purkinje cell zones and disrupts excitatory axon patterning. The distribution of pinceaux into size-specific zones is eliminated without Purkinje cell output. Our data uncover the cellular and molecular diversity of a foundational synapse that revolutionized neuroscience.

329 Synaptic responses between a cerebellar purkinje cell and parallel fibres by temporal pattern stimulation

1997 ◽  
Vol 28 ◽  
pp. S62
Author(s):  
Koutaro Torii ◽  
Takashi Kitsukawa ◽  
Raymond T. Kado ◽  
Kazuhiko Yamaguchi ◽  
Susumu Kunifuji ◽  
...  
Keyword(s):  
Purkinje Cell ◽  
Temporal Pattern ◽  
Cerebellar Purkinje Cell ◽  
Pattern Stimulation ◽  
Parallel Fibres ◽  
Synaptic Responses

scholarly journals Purkinje cell neurotransmission patterns cerebellar basket cells into zonal modules defined by distinct pinceau sizes

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Joy Zhou ◽  
Amanda M Brown ◽  
Elizabeth P Lackey ◽  
Marife Arancillo ◽  
Tao Lin ◽  
...  
Keyword(s):  
Purkinje Cell ◽  
Purkinje Cells ◽  
Initial Segment ◽  
Molecular Diversity ◽  
Basket Cell ◽  
Basket Cells ◽  
Conditional Allele ◽  
Functional Zones ◽  
Cell Somata ◽  
Ramón Y Cajal

Ramón y Cajal proclaimed the neuron doctrine based on circuit features he exemplified using cerebellar basket cell projections. Basket cells form dense inhibitory plexuses that wrap Purkinje cell somata and terminate as pinceaux at the initial segment of axons. Here, we demonstrate that HCN1, Kv1.1, PSD95 and GAD67 unexpectedly mark patterns of basket cell pinceaux that map onto Purkinje cell functional zones. Using cell-specific genetic tracing with an Ascl1CreERT2 mouse conditional allele, we reveal that basket cell zones comprise different sizes of pinceaux. We tested whether Purkinje cells instruct the assembly of inhibitory projections into zones, as they do for excitatory afferents. Genetically silencing Purkinje cell neurotransmission blocks the formation of sharp Purkinje cell zones and disrupts excitatory axon patterning. The distribution of pinceaux into size-specific zones is eliminated without Purkinje cell GABAergic output. Our data uncover the cellular and molecular diversity of a foundational synapse that revolutionized neuroscience.

Gap junctions in the prothoracic glands of the tobacco hornworm, Manduca sexta

1992 ◽  
Vol 50 (1) ◽  
pp. 706-707
Author(s):  
Ji-da Dai ◽  
M. Joseph Costello ◽  
Lawrence I. Gilbert
Keyword(s):  
Gap Junctions ◽  
Small Molecules ◽  
Manduca Sexta ◽  
Freeze Fracture ◽  
Cell Communication ◽  
Cellular Level ◽  
Thin Sections ◽  
Prothoracic Glands ◽  
Polyhydroxylated Steroids ◽  
Stimulation Of

Insect molting and metamorphosis are elicited by a class of polyhydroxylated steroids, ecdysteroids, that originate in the prothoracic glands (PGs). Prothoracicotropic hormone stimulation of steroidogenesis by the PGs at the cellular level involves both calcium and cAMP. Cell-to-cell communication mediated by gap junctions may play a key role in regulating signal transduction by controlling the transmission of small molecules and ions between adjacent cells. This is the first report of gap junctions in the PGs, the evidence obtained by means of SEM, thin sections and freeze-fracture replicas.

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