scholarly journals Osmunda pulchella sp. nov. from the Jurassic of Sweden—reconciling molecular and fossil evidence in the phylogeny of Osmundaceae

2014 ◽  
Author(s):  
Benjamin Bomfleur ◽  
Guido W Grimm ◽  
Stephen McLoughlin

The systematic classification of Osmundaceae has long remained controversial. Recent molecular data indicate that Osmunda is paraphyletic, and needs to be separated into Osmundastrum and Osmunda s. str. Here we describe an exquisitely preserved Jurassic Osmunda rhizome (O. pulchella sp. nov.) that combines diagnostic features of Osmundastrum and Osmunda, calling molecular evidence for paraphyly into question. We assembled a new morphological matrix based on rhizome anatomy, and used network analyses to establish phylogenetic relationships between fossil and extant members of modern Osmundaceae. We re-analysed the original molecular data to evaluate root-placement support. Finally, we integrated morphological and molecular data-sets using the evolutionary placement algorithm. Osmunda pulchella and five additional, newly identified Jurassic Osmunda species show anatomical character suites intermediate between Osmundastrum and Osmunda. Molecular evidence for paraphyly is ambiguous: a previously unrecognized signal from spacer sequences favours an alternative root placement that would resolve Osmunda s.l. as monophyletic. Our evolutionary placement analysis identifies fossil species as ancestral members of modern genera and subgenera. Altogether, the seemingly conflicting evidence from morphological, anatomical, molecular, and palaeontological data can be elegantly reconciled under the assumption that Osmunda is indeed monophyletic; the recently proposed root-placement in Osmundaceae—based solely on molecular data—likely results from un- or misinformative out-group signals.

1993 ◽  
Vol 24 (2) ◽  
pp. 121-137 ◽  
Author(s):  
Ward C. Wheeler ◽  
Ranhy Bang ◽  
Randall T. Schuh

AbstractThe monophyly of the 7 infraorders of Heteroptera and history of higher group concepts and interrelationships within the Heteroptera are briefly reviewed. Data from 31 morphological characters are combined with 669 bases of 18S nuclear rDNA for 29 taxa, including several outgroups to the Heteroptera, to produce a phylogeny based on the total available evidence. The molecular data alone and in conjunction with morphological data indicate that: the Homoptera are probably not monophyletic; the Auchenorrhyncha are the sister group of Coleorrhyncha + Heteroptera ; the Enicocephalomorpha are the sister group of remaining Heteroptera; the Dipsocoromorpha are the sister group of remaining Heteroptera; the Gerromorpha are the sister group of remaining Heteroptera; the Nepomorpha are the sister group of remaining Heteroptera; the Leptopodomorpha are the sister group of the Cimicomorpha + Pentatomomorpha. The molecular evidence corroborates the morphologically based theory of a sister group relationship between Aradoidea and trichophoran Pentatomomorpha. This scheme deviates from that previously published by Schuh, in which the Leptopodomorpha were treated as the sister group of the Nepomorpha.


Zootaxa ◽  
2004 ◽  
Vol 680 (1) ◽  
pp. 1 ◽  
Author(s):  
ARNE NYGREN

Autolytinae is revised based on available types, and newly collected specimens. Out of 170 nominal species, 18 are considered as incertae sedis, 43 are regarded as junior synonyms, and 25 are referred to as nomina dubia. The relationships of Autolytinae is assessed from 51 morphological characters and 211 states for 76 ingroup-taxa, and 460 molecular characters from mitochondrial 16S rDNA and nuclear 18S rDNA for 31 ingroup-taxa; outgroups include 12 non-autolytine syllid polychaetes. Two analyses are provided, one including morphological data only, and one with combined morphological and molecular data sets. The resulting strict consensus tree from the combined data is chosen for a reclassification. Three main clades are identified: Procerini trib. n., Autolytini Grube, 1850, and Epigamia gen. n. Proceraea Ehlers, 1864 and Myrianida Milne Edwards, 1845 are referred to as nomen protectum, while Scolopendra Slabber, 1781, Podonereis Blainville, 1818, Amytis Savigny, 1822, Polynice Savigny, 1822, and Nereisyllis Blainville, 1828 are considered


Phytotaxa ◽  
2019 ◽  
Vol 388 (4) ◽  
pp. 275 ◽  
Author(s):  
BEATRIZ BRUNELLI ◽  
DANIELA MILSTEIN ◽  
SUNG M. BOO ◽  
MUTUE T. FUJII

The Gelidium species are susceptible to a great phenotypic plasticity. In this genus, studies integrating morphological and molecular data have been increasingly used to define species. To date nine Gelidium species are reported along the Brazilian coast. Gelidium floridanum is the most frequently recorded species in Brazilian but it is also often confused with morphologically similar species, especially in the field or when reproductive structures are absent. In this study we analyzed specimens from Brazil previously identified as G. floridanum based on the morphological and molecular data (cox1 and rbcL DNA sequences). Newly collected specimens from Brazil showed a DNA sequence divergence from G. floridanum in the order of 1.0–1.4% for rbcL and 4.2% for cox1, enough to be considered a new species, here described as Gelidium guimaraesiae sp. nov.


2021 ◽  
Author(s):  
E. J. Thompson ◽  
Melodina Fabillo

The taxonomy of Neurachninane has been unstable, with its member genera consisting of Ancistrachne, Calyptochloa, Cleistochloa, Dimorphochloa, Neurachne, Paraneurachne and Thyridolepis, changing since its original circumscription that comprised only the latter three genera. Recent studies on the phylogeny of Neurachninae have focused primarily on molecular data. We analysed the phylogeny of Neurachninae on the basis of molecular data from seven molecular loci (plastid markers: matK, ndhF, rbcL, rpl16, rpoC2 and trnLF, and ribosomal internal transcribed spacer, ITS) and morphological data from 104 morphological characters, including new taxonomically informative micromorphology of upper paleas. We devised an impact assessment scoring (IAS) protocol to aid selection of a tree for inferring the phylogeny of Neurachninae. Combining morphological and molecular data resulted in a well resolved phylogeny with the highest IAS value. Our findings support reinstatement of subtribe Neurachninae in its original sense, Neurachne muelleri and Dimorphochloa rigida. We show that Ancistrachne, Cleistochloa and Dimorphochloa are not monophyletic and Ancistrachne maidenii, Calyptochloa, Cleistochloa and Dimorphochloa form a new group, the cleistogamy group, united by having unique morphology associated with reproductive dimorphism.


2010 ◽  
Vol 41 (3) ◽  
pp. 231-274 ◽  
Author(s):  
Jinzhong Fu ◽  
Owen Lonsdale ◽  
Brian Wiegmann ◽  
Stephen Marshall

AbstractIn this paper, the Clusiidae (Diptera: Schizophora) is analyzed phylogenetically using morphological and molecular data sets, and then redefined on the basis of derived morphological characters. The biology and distribution of the Clusiidae are also reviewed, a key is provided to the World genera, the status of the genus Craspedochaeta Czerny is reevaluated and the type of Heterochroa pictipennis Wulp is discussed. Molecular data sets include genomic DNA sequences from the mitochondrial genes COI (cytochrome oxidase subunit I) and COII (cytochrome oxidase subunit II), the large ribosomal nuclear subunit 28S, and the nuclear protein-coding carbomoylphosphate synthase (CPS) domain of CAD (or “rudimentary”). Genes were analyzed separately, in combination with each other, and in combination with a morphological data set. Although individual molecular data sets often provided conflicting phylogenetic signals, the topologies of the cladograms produced from each data set alone or in combination were largely similar. Most genus-level relationships and several basal divergences were unresolved, but Apiochaeta was very strongly and consistently supported as Sobarocephalinae, not Clusiinae. The Clusiinae and Sobarocephalinae are subsequently redefined using an adjusted morphological tree — retaining Apiochaeta in the Sobarocephalinae — that is only slightly longer (8.4%, or seven steps) than the most parsimonious tree. Our results illustrate the benefits of multiple independent data sets for phylogenetic reconstruction in order to verify and refine existing classifications.


MycoKeys ◽  
2020 ◽  
Vol 63 ◽  
pp. 163-172
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.


Zootaxa ◽  
2007 ◽  
Vol 1668 (1) ◽  
pp. 591-638 ◽  
Author(s):  
RALPH E. HARBACH

The taxonomy, classification and phylogeny of family Culicidae are reviewed. The application of explicit methods of phylogenetic analysis has revealed weaknesses in the traditional classification of mosquitoes, but little progress has been made to achieve a robust, stable classification that reflects evolutionary relationships. The current phenetic classification is discussed in view of phylogeny reconstructions based on cladistic analyses of morphological and molecular data. It is concluded that the generic and suprageneric relationships and the validity and monophyly of the generic and subgeneric groupings of Culicidae are in need of extensive reappraisal. If the classification is to reflect evolutionary history, changes to the nomenclature of mosquitoes are inevitable. There is strong morphological and molecular evidence that subfamily Anophelinae and tribes Aedini, Culicini and Sabethini of subfamily Culicinae are monophyletic, but the other taxonomic groupings are not demonstrably monophyletic or have not been subjected to phylogenetic analyses.


MycoKeys ◽  
2019 ◽  
Vol 61 ◽  
pp. 1-26
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.


Phytotaxa ◽  
2021 ◽  
Vol 500 (1) ◽  
pp. 45-50
Author(s):  
XIA YU ◽  
MENG-YAO ZENG ◽  
GUI-ZHEN CHEN ◽  
XIN-YU XU ◽  
YUAN-YANG BI ◽  
...  

A new species of Cymbidium (Orchidaceae), C. weishanense, from Yunnan Province, China, is illustrated and described based on morphological and molecular evidence. Morphological comparison indicates that C. weishanense is similar to C. concinnum but differs in several floral characteristics. Molecular analyses based on nuclear ribosomal (ITS) and plastid DNA (matK and rbcL) support the status of C. weishanense as a new species. Due to the low support for nrITS, it is possible that this is a natural hybrid of C. concinnum with C. insigne or another related species.


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