scholarly journals Bacillus subtilis spore surface display of photodecarboxylase for the transformation of lipids to hydrocarbons

2020 ◽  
Author(s):  
Marianna Karava ◽  
Peter Gockel ◽  
Johannes Kabisch
Keyword(s):  
Bacillus Subtilis ◽  
Immobilized Lipase ◽  
Surface Display ◽  
Immobilized Enzymes ◽  
Spore Surface ◽  
Oleaginous Yeasts ◽  
Biotechnological Production ◽  
One Pot ◽  
Bacillus Subtilis Spore ◽  
3D Printed

AbstractThe display of enzymes on the surface of spores allows the rapid and very simple biotechnological production of immobilized enzymes. Here we describe the development of a Bacillus subtilis spore display platform and its application to produce hydrocarbons from lipids obtained from the oleaginous yeasts Yarrowia lipolytica, Cutaneotrichosporon oleaginosus as well as olive oil.Lipid hydrolysis was examined in a bienzymatic one-pot cascade using a commercially immobilized lipase (RO lipase) as well as spores with and without additional heterologous lipase expression. Decarboxylation of the released fatty acids was achieved displaying a photodecarboxylase (CvFAP) on the spore surface. Differences in composition of the formed hydrocarbons were observed depending on the lipids source. Using 3D printed lighting equipment titers of up to 64.0 ± 5.6 mg/L hydrocarbons were produced.

Bacillus subtilis Spore Surface Display of Haloalkane Dehalogenase DhaA

2019 ◽  
Vol 76 (10) ◽  
pp. 1161-1167 ◽  
Author(s):  
Fuli Wang ◽  
Tianyu Song ◽  
Hui Jiang ◽  
Chengxin Pei ◽  
Qibin Huang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Haloalkane Dehalogenase ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display

scholarly journals Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

2019 ◽  
Vol 29 (2) ◽  
pp. 179-190 ◽  
Author(s):  
Zhang Guoyan ◽  
An Yingfeng ◽  
Hossain M Zabed ◽  
Guo Qi ◽  
Miaomiao Yang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display ◽  
Display Technology

scholarly journals A system of vectors for Bacillus subtilis spore surface display

2014 ◽  
Vol 13 (1) ◽  
pp. 30 ◽  
Author(s):  
Adam Iwanicki ◽  
Iwona Piątek ◽  
Małgorzata Stasiłojć ◽  
Anna Grela ◽  
Tomasz Łęga ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display

scholarly journals Bacillus subtilis spore surface display of photodecarboxylase for the transformation of lipids to hydrocarbons

2021 ◽  
Vol 5 (6) ◽  
pp. 1727-1733
Author(s):  
Marianna Karava ◽  
Peter Gockel ◽  
Johannes Kabisch
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Efficient Strategy ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display ◽  
Cost Efficient

We suggest spore display as a simple and cost efficient strategy for the production of immobilized photodecarboxylase utilized for the conversion of oils to biofuels.

scholarly journals Assembly of Multiple CotC Forms into the Bacillus subtilis Spore Coat

2004 ◽  
Vol 186 (4) ◽  
pp. 1129-1135 ◽  
Author(s):  
Rachele Isticato ◽  
Giovanni Esposito ◽  
Rita Zilhão ◽  
Sofia Nolasco ◽  
Giuseppina Cangiano ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Posttranslational Modifications ◽  
Mother Cell ◽  
Spore Coat ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Cell Compartment ◽  
Bacillus Subtilis Spore ◽  
Molecular Masses

ABSTRACT We report evidence that the CotC polypeptide, a previously identified component of the Bacillus subtilis spore coat, is assembled into at least four distinct forms. Two of these, having molecular masses of 12 and 21 kDa, appeared 8 h after the onset of sporulation and were probably assembled on the forming spore immediately after their synthesis, since no accumulation of either of them was detected in the mother cell compartment, where their synthesis occurs. The other two components, 12.5 and 30 kDa, were generated 2 h later and were probably the products of posttranslational modifications of the two early forms occurring directly on the coat surface during spore maturation. None of the CotC forms was found either on the spore coat or in the mother cell compartment of a cotH mutant. This indicates that CotH serves a dual role of stabilizing the early forms of CotC and promoting the assembly of both early and late forms on the spore surface.

scholarly journals Removal of Ni2+ and Cd2+ by Surface Display of Polyhistidine on Bacillus subtilis Spore Using CotE Anchor Protein

2019 ◽  
Vol 24 (2) ◽  
pp. 375-381 ◽  
Author(s):  
Wooil Kim ◽  
Daeun Kim ◽  
Sanggeun Back ◽  
Yong-suk Lee ◽  
Afrouzossadat Hosseini Abari ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Subtilis Spore ◽  
Anchor Protein ◽  
Bacillus Subtilis Spore
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