scholarly journals Effect of Biologically Active Hydrogen-Generating Powder Mixed with Food in a Model of Liver Injury in Rats

2021 ◽  
Author(s):  
Bronislava Gedulin ◽  
Marina Safonov ◽  
Vladimir L. Safonov
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Total Bilirubin ◽  
Bile Duct Ligation ◽  
Total Bilirubin Level ◽  
Biologically Active ◽  
Vehicle Group ◽  
Control Group ◽  
Good Efficacy ◽  
Duct Ligation

ABSTRACTBackgroundHydrogen was determined to have good efficacy for reducing key blood level biomarkers associated with liver injury suggesting the compound may provide novel option for the treatment of several liver diseases by decreasing of accumulation of toxins and reduction of levels of serum liver enzymes.Materials and methodsThe present pharmacological study was conducted to evaluate the effectiveness of a gastric hydrogen generating powder called “AquaActive” (AA) in a rat model of liver injury, the partial bile duct ligation (pBDL), following administration of 0.2% AA formulated in rat pellets food for 14 days.ResultsThe data indicate that treatment with AA at a dose of 150 mg/kg/day can effectively slow the progression of liver injury that is triggered by bile duct ligation in rats. At both 7 and 14 days post-pBDL surgery, treatment with AA exhibited reductions in ALT, AST, ALP, GGT and total bilirubin, most of which were statistically significant. At 7 days, the compound showed statistically significant decreases in ALP, GGT and total bilirubin levels. Although the values of some parameters decreased in the vehicle group by 14 days, additional reductions due to AA treatment were sustained for ALP, AST and for GGT and total bilirubin. GGT and total bilirubin level after 14 days of treatment compared to the vehicle-treated control group were observed to be highly significant (p<0.05).ConclusionThus AA, gastric hydrogen generating powder demonstrated a good efficacy for reducing key parameters associated with liver function in the pBDL model.

Bid-mediated apoptosis does not contribute to cholestatic liver injury following bile duct ligation

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
P Nalapareddy ◽  
S Schüngel ◽  
MP Manns ◽  
H Jaeschke ◽  
A Vogel
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Bile Duct Ligation ◽  
Duct Ligation ◽  
Cholestatic Liver Injury

scholarly journals Vitamin A Supplementation Alleviates Extrahepatic Cholestasis Liver Injury through Nrf2 Activation

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Guiyang Wang ◽  
Peng Xiu ◽  
Fu Li ◽  
Cheng Xin ◽  
Kewei Li
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Liver Function ◽  
Vitamin A ◽  
Western Blotting ◽  
Bile Duct Ligation ◽  
Retinyl Palmitate ◽  
Binding Activity ◽  
Extrahepatic Cholestasis ◽  
Duct Ligation

Aim. To investigate the role of vitamin A in liver damage induced by bile duct ligation (BDL) in rats.Methods. Thirty male Wistar rats were randomly divided into three groups: SHAM group, BDL group, and BDL + VitA group . The concentrations of retinol and retinyl palmitate in the liver were analyzed using HPLC, and liver function was evaluated by the level of TBIL, ALT, AST, and ALP in serum. Hepatic oxidative status was estimated by measuring T-SOD, CAT, GSH, MDA, and AOPP. Nrf2 expression was assessed using immunohistochemistry and western blotting, and EMSA was performed to determine Nrf2 DNA-binding activity. The expression of the downstream factors such as Ho1 and Nqo1 was also examined using immunohistochemistry and western blotting assays.Results. Vitamin A treatment restored levels of retinoids in liver, improved liver function, alleviated oxidative stress, and facilitated the translocation of Nrf2 to the nucleus in the experimental obstructive jaundice. Vitamin A was also found to increase the expression of Nrf2 downstream proteins such as Ho1 and Nqo1.Conclusion. Vitamin A was here found to ameliorate cholestatic liver injury. This effect may be related to the activation of Nrf2/ARE pathway in bile duct ligation rats.

scholarly journals Cell-specific regulatory effects of CXCR2 on cholestatic liver injury

2019 ◽  
Vol 317 (6) ◽  
pp. G773-G783 ◽  
Author(s):  
Takanori Konishi ◽  
Rebecca M. Schuster ◽  
Holly S. Goetzman ◽  
Charles C. Caldwell ◽  
Alex B. Lentsch
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Liver Damage ◽  
Chemokine Receptor ◽  
Therapeutic Target ◽  
Bile Duct Ligation ◽  
Wild Type ◽  
Neutrophil Accumulation ◽  
Duct Ligation ◽  
Cholestatic Liver Injury

The CXC chemokine receptor 2 (CXCR2) is critical for neutrophil recruitment and hepatocellular viability but has not been studied in the context of cholestatic liver injury following bile duct ligation (BDL). The present study sought to elucidate the cell-specific roles of CXCR2 on acute liver injury after BDL. Wild-type and CXCR2−/− mice were subjected BDL. CXCR2 chimeric mice were created to assess the cell-specific role of CXCR2 on liver injury after BDL. SB225002, a selective CXCR2 antagonist, was administrated intraperitoneally after BDL to investigate the potential of pharmacological inhibition. CXCR2−/− mice had significantly less liver injury than wild-type mice at 3 and 14 days after BDL. There was no difference in biliary fibrosis among groups. The chemokines CXCL1 and CXCL2 were induced around areas of necrosis and biliary structures, respectively, both areas where neutrophils accumulated after BDL. CXCR2−/− mice showed significantly less neutrophil accumulation in those injured areas. CXCR2Liver+/Myeloid+ and CXCR2Liver−/Myeloid− mice recapitulated the wild-type and CXCR2-knockout phenotypes, respectively. CXCR2Liver+/Myeloid+ mice suffered higher liver injury than CXCR2Liver+/Myeloid− and CXCR2Liver−/Myeloid+; however, only those chimeras with knockout of myeloid CXCR2 (CXCR2Liver+/Myeloid− and CXCR2Liver−/Myeloid−) showed reduction of neutrophil accumulation around areas of necrosis. Daily administration of SB225002 starting after 3 days of BDL reduced established liver injury at 6 days. In conclusion, neutrophil CXCR2 guides the cell to the site of injury, while CXCR2 on liver cells affects liver damage independent of neutrophil accumulation. CXCR2 appears to be a viable therapeutic target for cholestatic liver injury. NEW & NOTEWORTHY This study is the first to reveal cell-specific roles of the chemokine receptor CXCR2 in cholestatic liver injury caused by bile duct ligation. CXCR2 on neutrophils facilitates neutrophil recruitment to the liver, while CXCR2 on liver cells contributes to liver damage independent of neutrophils. CXCR2 may represent a viable therapeutic target for cholestatic liver injury.

scholarly journals C/EBP homologous protein-induced loss of intestinal epithelial stemness contributes to bile duct ligation-induced cholestatic liver injury in mice

Hepatology ◽  
2018 ◽  
Vol 67 (4) ◽  
pp. 1441-1457 ◽  
Author(s):  
Runping Liu ◽  
Xiaojiaoyang Li ◽  
Zhiming Huang ◽  
Derrick Zhao ◽  
Bhagyalaxmi Sukka Ganesh ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Bile Duct Ligation ◽  
Intestinal Epithelial ◽  
Homologous Protein ◽  
Duct Ligation ◽  
Cholestatic Liver Injury

scholarly journals Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

10.3791/52438 ◽  
2015 ◽  
Author(s):  
Carmen G. Tag ◽  
Sibille Sauer-Lehnen ◽  
Sabine Weiskirchen ◽  
Erawan Borkham-Kamphorst ◽  
René H. Tolba ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Bile Duct Ligation ◽  
Duct Ligation ◽  
Obstructive Cholestasis

Cholestatic liver injury model of bile duct ligation and the protection of Huang-Lian-Jie-Du decoction by NMR metabolomic profiling

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 66200-66211 ◽  
Author(s):  
Dandan Wei ◽  
Shanting Liao ◽  
Junsong Wang ◽  
Minghua Yang ◽  
Lingyi Kong
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Treatment Effects ◽  
Bile Duct Ligation ◽  
Metabolomic Profiling ◽  
Injury Model ◽  
Duct Ligation ◽  
Cholestatic Liver Injury

Bile duct ligation (BDL) induced cholestasis in rats and the treatment effects of Huang-Lian-Jie-Du decoction (HLJDD) were investigated by NMR-based metabolomics approach: biphasic feature of BDL model and bilateral adjustment of HLJDD were found.

scholarly journals Ascorbate lacks significant influence in rats with bile duct ligation-induced liver injury

2017 ◽  
Vol 80 (9) ◽  
pp. 539-550 ◽  
Author(s):  
Hsin-Ling Ho ◽  
Teh-Ia Huo ◽  
Ting Chang ◽  
Wen-Shin Lee ◽  
I-Fang Hsin ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Significant Influence ◽  
Bile Duct Ligation ◽  
Duct Ligation
Sign in / Sign up

Export Citation Format

Share Document