Olfactory receptor-dependent receptor repression in Drosophila

AbstractIn olfactory systems across phyla, most sensory neurons transcribe a single olfactory receptor gene selected from a large genomic repertoire. We describe novel receptor gene-dependent mechanisms that ensure singular expression of receptors encoded by a tandem gene array in Drosophila. Transcription from upstream genes in the cluster runs through the coding region of downstream loci to inhibit their expression in cis, via transcriptional interference. Moreover, one receptor blocks expression of other receptor proteins in trans through a post-transcriptional mechanism. These repression mechanisms operate in endogenous neurons to ensure their unique expression. Our data provide evidence for inter-olfactory receptor regulation in invertebrates, and highlight unprecedented, but potentially widespread, mechanisms for ensuring exclusive expression of chemosensory receptors, and other protein families, encoded by tandemly-arranged genes.

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Olfactory receptor–dependent receptor repression in Drosophila

Science Advances ◽

10.1126/sciadv.abe3745 ◽

2021 ◽

Vol 7 (32) ◽

pp. eabe3745

Author(s):

Kaan Mika ◽

Steeve Cruchet ◽

Phing Chian Chai ◽

Lucia L. Prieto-Godino ◽

Thomas O. Auer ◽

...

Keyword(s):

Olfactory Receptor ◽

Regulatory Networks ◽

Receptor Gene ◽

Gene Array ◽

Receptor Expression ◽

Specific Gene ◽

Coding Region ◽

Ionotropic Receptor ◽

Olfactory Systems ◽

In Cis

In olfactory systems across phyla, most sensory neurons express a single olfactory receptor gene selected from a large genomic repertoire. We describe previously unknown receptor gene–dependent mechanisms that ensure singular expression of receptors encoded by a tandem gene array [Ionotropic receptor 75c (Ir75c), Ir75b, and Ir75a, organized 5′ to 3′] in Drosophila melanogaster. Transcription from upstream genes in the cluster runs through the coding region of downstream loci and inhibits their expression in cis, most likely via transcriptional interference. Moreover, Ir75c blocks accumulation of other receptor proteins in trans through a protein-dependent, posttranscriptional mechanism. These repression mechanisms operate in endogenous neurons, in conjunction with cell type–specific gene regulatory networks, to ensure unique receptor expression. Our data provide evidence for inter-olfactory receptor regulation in invertebrates and highlight unprecedented, but potentially widespread, mechanisms for ensuring exclusive expression of chemosensory receptors, and other protein families, encoded by tandemly arranged genes.

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Patterned Arrangements of Olfactory Receptor Gene Expression in Zebrafish are Established by Radial Movement of Specified Olfactory Sensory Neurons

Scientific Reports ◽

10.1038/s41598-017-06041-1 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 7

Author(s):

Xalid Bayramli ◽

Yiğit Kocagöz ◽

Uğurcan Sakizli ◽

Stefan H. Fuss

Keyword(s):

Gene Expression ◽

Sensory Neurons ◽

Olfactory Receptor ◽

Receptor Gene ◽

Olfactory Sensory Neurons ◽

Olfactory Receptor Gene ◽

Radial Movement ◽

Receptor Gene Expression

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Faculty Opinions recommendation of The olfactory receptor gene superfamily of the mouse.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004627.52854 ◽

2002 ◽

Author(s):

Peter Mombaerts

Keyword(s):

Olfactory Receptor ◽

Receptor Gene ◽

Olfactory Receptor Gene

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Olfactory receptor gene repertoires in mammals

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/j.mrfmmm.2006.11.012 ◽

2007 ◽

Vol 616 (1-2) ◽

pp. 95-102 ◽

Cited By ~ 38

Author(s):

Sylvie Rouquier ◽

Dominique Giorgi

Keyword(s):

Olfactory Receptor ◽

Receptor Gene ◽

Olfactory Receptor Gene

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Differing from diseased stage in melanoma, the olfactory receptor gene OR56A4 is expressed

10.31219/osf.io/yz37x ◽

2021 ◽

Author(s):

Bronte Morse ◽

Kobi Decker

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Olfactory Receptor ◽

Receptor Gene ◽

Stage I ◽

Stage Ii ◽

Stage Iii ◽

Olfactory Receptor Gene ◽

Breast Cancer Patients ◽

Receptor Family

We have compared the global profiles of 100 tumors in Stage I, II and III with two independently releasedmicroarray datasets in order to understand their transcriptional behaviors accompanying a progression in breastcancer (1, 2). The olfactive receptor, family 56, subfamily A, member 4 OR56A4, was discovered to have beenone of the genes with the most varied expression when comparing initial tumors in stage I, stage II, and stageIII of breast cancer patients. In the stage III tumors, OR56A4 expression in comparison to the stage I tumorswas lower.

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