scholarly journals AN ECONOMIC, SQUARE-SHAPED FLAT-FIELD ILLUMINATION MODULE FOR TIRF-BASED SUPER-RESOLUTION MICROSCOPY

2021 ◽  
Author(s):  
Jeff Y L Lam ◽  
Yunzhao Wu ◽  
Eleni Dimou ◽  
Ziwei Zhang ◽  
Matthew R Cheetham ◽  
...  
Keyword(s):  
High Throughput ◽  
Total Internal Reflection ◽  
Quantitative Imaging ◽  
Super Resolution ◽  
Cost Effective ◽  
Cellular Systems ◽  
Cost Effective Method ◽  
Flat Field ◽  
Super Resolution Microscopy ◽  
Highly Uniform

Super-resolution (SR) microscopy allows complex biological assemblies to be observed with remarkable resolution. However, the presence of uneven Gaussian-shaped illumination hinders its use in quantitative imaging or high-throughput assays. Methods developed to circumvent this problem are often expensive, hard-to-implement, or not applicable to total internal reflection fluorescence (TIRF) imaging. We herein demonstrate a cost-effective method to overcome these challenges using a small square-core multimodal optical fibre as the coupler. We characterise our method with synthetic, recombinant and cellular systems imaged under TIRF and highly inclined and laminated optical sheet (HILO) illuminations to demonstrate its ability to produce highly uniform images under all conditions.

scholarly journals 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples

2019 ◽  
Author(s):  
Ruisheng Lin ◽  
Alexander H. Clowsley ◽  
Tobias Lutz ◽  
David Baddeley ◽  
Christian Soeller
Keyword(s):  
Quantitative Imaging ◽  
Super Resolution ◽  
Cost Effective ◽  
Dna Origami ◽  
Robust Test ◽  
Imaging Quality ◽  
Multiple Test ◽  
Resolution Imaging ◽  
2D Imaging ◽  
Super Resolution Microscopy

AbstractAssessment of the imaging quality in localisation-based super-resolution techniques relies on an accurate characterisation of the imaging setup and analysis procedures. Test samples can provide regular feedback on system performance and facilitate the implementation of new methods. While multiple test samples for regular, 2D imaging are available, they are not common for more specialised imaging modes. Here, we analyse robust test samples for 3D and quantitative super-resolution imaging, which are straightforward to use, are time-and cost-effective and do not require experience beyond basic laboratory and imaging skills. We present two options for assessment of 3D imaging quality, the use of microspheres functionalised for DNA-PAINT and a commercial DNA origami sample. A method to establish and assess a qPAINT workflow for quantitative imaging is demonstrated with a second, commercially available DNA origami sample.

scholarly journals Simple, Fast, and Cost-Effective Fabrication of Wafer-Scale Nanohole Arrays on Silicon for Antireflection

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Di Di ◽  
Xuezhong Wu ◽  
Peitao Dong ◽  
Chaoguang Wang ◽  
Jian Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Crystalline Silicon ◽  
Colloidal Crystal ◽  
Cost Effective ◽  
Wafer Scale ◽  
Crystalline Silicon Solar Cells ◽  
Cost Effective Method ◽  
Nanohole Arrays ◽  
Coating Method ◽  
Hole Arrays

A simple, fast, and cost-effective method was developed in this paper for the high-throughput fabrication of nanohole arrays on silicon (Si), which is utilized for antireflection. Wafer-scale polystyrene (PS) monolayer colloidal crystal was developed as templates by spin-coating method. Metallic shadow mask was prepared by lifting off the oxygen etched PS beads from the deposited chromium film. Nanohole arrays were fabricated by Si dry etching. A series of nanohole arrays were fabricated with the similar diameter but with different depth. It is found that the maximum depth of the Si-hole was determined by the diameter of the Cr-mask. The antireflection ability of these Si-hole arrays was investigated. The results show that the reflection decreases with the depth of the Si-hole. The deepest Si-hole arrays show the best antireflection ability (reflection < 9%) at long wavelengths (>600 nm), which was about 28 percent of the nonpatterned silicon wafer’s reflection. The proposed method has the potential for high-throughput fabrication of patterned Si wafer, and the low reflectivity allows the application of these wafers in crystalline silicon solar cells.

scholarly journals Photonic chip-based multimodal super-resolution microscopy for histopathological assessment of cryopreserved tissue sections

2021 ◽  
Author(s):  
Luis E. Villegas-Hernandez ◽  
Vishesh Kumar Dubey ◽  
Mona Nystad ◽  
Jean-Claude Tinguely ◽  
David A. Coucheron ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Molecule ◽  
Operating Cost ◽  
Super Resolution ◽  
Anatomical Variations ◽  
Histopathological Analysis ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Imaging Tool ◽  
Super Resolution Microscopy

Histopathological assessment involves the identification of anatomical variations in tissues that are associated with diseases. While diffraction-limited optical microscopes assist in the diagnosis of a wide variety of pathologies, their resolving capabilities are insufficient to visualize some anomalies at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the system complexity, high operating cost, lack of multimodality, and low-throughput imaging of these methods limit their wide adoption in clinical settings. In this study, we interrogate the photonic chip as an attractive high-throughput super-resolution microscopy platform for histopathology. Using cryopreserved ultrathin tissue sections of human placenta, mouse kidney, and zebrafish eye retina prepared by the Tokuyasu method, we validate the photonic chip as a multi-modal imaging tool for histo-anatomical analysis. We demonstrate that photonic-chip platform can deliver multi-modal imaging capabilities such as total internal reflection fluorescence microscopy, intensity fluctuation-based optical nanoscopy, single-molecule localization microscopy, and correlative light-electron microscopy. Our results demonstrate that the photonic chip-based super-resolution microscopy platform has the potential to deliver high-throughput multimodal histopathological analysis of cryopreserved tissue samples.

scholarly journals Genetic Analysis of colorectal carcinoma using high throughput SNP genotyping technique within the population of Jammu and Kashmir

2021 ◽  
Author(s):  
Bhanu Sharma ◽  
Shabab Angurana ◽  
Amrita Bhat ◽  
Sonali Verma ◽  
Divya Bakshi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Throughput ◽  
Cost Effective ◽  
Snp Genotyping ◽  
Colorectal Carcinogenesis ◽  
P Value ◽  
Jammu And Kashmir ◽  
Cost Effective Method ◽  
Underlying Causes ◽  
Multiple Samples

Abstract Background SNP genotyping has become increasingly more common place to understand the genetic basis of complex diseases like cancer. SNP-genotyping through massARRAY is a cost-effective method to quantitatively analyse the variation of gene expression in multiple samples, making it a potential tool to identify the underlying causes of colorectal carcinogenesis. Methods In the present study, SNP genotyping was carried out using Agena mass ARRAY, which is a cost-effective, robust, and sensitive method to analyse multiple SNPs simultaneously. We analysed 7 genes in 492 samples (100 cases and 392 controls) associated with CRC within the population of Jammu and Kashmir. These SNPs were selected based on their association with multiple cancers in literature. Results This is the first study to explore these SNPs with colorectal cancer within the J&K population.7 SNPs with a call rate of 90% were selected for the study. Out of these, one SNP i.e. rs2229080 of DCC was found to be significantly associated with the current study and 6 were non-significantly associated with CRC within the studied population. The allelic OR observed for the variant rs2229080 of DCC was 1.5 (1.1–2.3 at 95% CI), p value = 0.02. Conclusion This is the first study to find the relation of Genetic variants with the colorectal cancer within the studied population using high throughput mass ARRAY technology. It is further anticipated that the variants should be evaluated in other population groups that may aid in understanding the genetic complexity and bridge the missing heritability.

Wire mask assisted rolling as a cost-effective method for high-throughput surface micro-texturing

2020 ◽  
Vol 30 (7) ◽  
pp. 075010
Author(s):  
Anvesh Gaddam ◽  
Ashwin Prabhakaran ◽  
Amit Agrawal ◽  
Suhas S Joshi
Keyword(s):  
High Throughput ◽  
Cost Effective ◽  
Cost Effective Method

Single-molecule fluorescence imaging by total internal reflection fluorescence microscopy (IUPAC Technical Report)

2014 ◽  
Vol 86 (8) ◽  
pp. 1303-1320 ◽  
Author(s):  
Alex E. Knight
Keyword(s):  
Single Molecule ◽  
Total Internal Reflection ◽  
Super Resolution ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Practical Implementation ◽  
Sequencing Technologies ◽  
Technical Report ◽  
High Throughput Dna Sequencing ◽  
Super Resolution Microscopy

AbstractTotal internal reflection fluorescence (TIRF) is a popular illumination technique in microscopy, with many applications in cell and molecular biology and biophysics. The chief advantage of the technique is the high contrast that can be achieved by restricting fluorescent excitation to a thin layer. We summarise the optical theory needed to understand the technique and various aspects required for a practical implementation of it, including the merits of different TIRF geometries. Finally, we discuss a variety of applications including super-resolution microscopy and high-throughput DNA sequencing technologies.

Sign in / Sign up

Export Citation Format

Share Document