Simultaneous Optimization of Excitation Wavelength and Emission Window for Semiconducting Polymer Nanoparticles to Improve Imaging Quality

Optimized excitation wavelength and emission window are essential for fluorescence imaging with high quality. Semiconducting polymer nanoparticles (SPNs) as fluorescent contrast agents have been extensively studied, but their imaging abilities in the second near infrared IIb window (NIR-IIb, 1500 to 1700 nm) with long excitation wavelength have not been reported yet. Herein, as a proof-of-concept, we demonstrate for the first time that an SPN named L1057 nanoparticles (NPs) exhibit intense NIR-IIb signal due to its ultra-high brightness and broad emission spectrum. After screening 915 nm as an optimal excitation wavelength, we applied L1057 NPs to visualize the whole body vessels, cerebral vessels, gastrointestinal tract, and tumor progression in different stages, achieving superior spatial resolution and signal to background ratio in the NIR-IIb window with respect to NIR-II window (1000 to 1700 nm). This study reveals that simultaneous optimization of excitation wavelength and emission window is an efficient strategy to enhance imaging quality and that L1057 NPs can serve as a promising NIR-IIb contrast agent for high-resolution and deep-tissue imaging.

Analysis of Hypersonic Platform-Borne SAR Imaging: A Physical Perspective

The usage of a hypersonic platform for remote sensing application has promising prospects, especially for hypersonic platform-borne synthetic aperture radar (SAR) imaging. However, the high-speed of hypersonic platform will lead to extreme friction between the platform and air, which will cause the ionization of air. The ionized gas forms the plasma sheath wrapped around the hypersonic platform. The plasma sheath will severely affect the propagation of SAR signal and further affect the SAR imaging. Therefore, hypersonic platform-borne SAR imaging should be studied from a physical perspective. In this paper, hypersonic platform-borne SAR imaging under plasma sheath is analyzed. The SAR signal propagation in plasma sheath is computed using scatter matrix method. The proposed SAR signal model is verified by using a ground experiment system. Moreover, the effect of attenuation caused by plasma sheath on SAR imaging is studied under different SAR parameters and plasma sheath. The result shows that attenuation caused by plasma sheath will degrade the SAR imaging quality and even cause the point and area targets to be submerged into the noise. The real SAR images under plasma sheath also illustrate this phenomenon. Furthermore, by studying imaging results under different SAR and plasma parameters, it can be concluded that the severe degradation of SAR imaging quality appears at condition of high plasma sheath electron density and low SAR carrier frequency. The work in this paper will be beneficial for the study of hypersonic platform-borne SAR imaging and design of hypersonic SAR imaging systems in the future.

Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration

Laser speckle contrast imaging (LSCI) is a powerful tool to monitor blood flow distribution and has been widely used in studies of microcirculation, both for animal and clinical applications. Conventionally, LSCI usually works on reflective-detected mode. However, it could provide promising temporal and spatial resolution for in vivo applications only with the assistance of various tissue windows, otherwise, the overlarge superficial static speckle would extremely limit its contrast and resolution. Here, we systematically investigated the capability of transmissive-detected LSCI (TR-LSCI) for blood flow monitoring in thick tissue. Using Monte Carlo simulation, we theoretically compared the performance of transmissive and reflective detection. It was found that the reflective-detected mode was better when the target layer was at the very surface, but the imaging quality would rapidly decrease with imaging depth, while the transmissive-detected mode could obtain a much stronger signal-to-background ratio (SBR) for thick tissue. We further proved by tissue phantom, animal, and human experiments that in a certain thickness of tissue, TR-LSCI showed remarkably better performance for thick-tissue imaging, and the imaging quality would be further improved if the use of longer wavelengths of near-infrared light. Therefore, both theoretical and experimental results demonstrate that TR-LSCI is capable of obtaining thick-tissue blood flow information and holds great potential in the field of microcirculation research.

Near-Surface Geological Structure Seismic Wave Imaging Using the Minimum Variance Spatial Smoothing Beamforming Method

Erecting underground structures in regions with unidentified weak layers, cavities, and faults is highly dangerous and potentially disastrous. An efficient and accurate near-surface exploration method is thus of great significance for guiding construction. In near-surface detection, imaging methods suffer from artifacts that the complex structure caused and a lack of efficiency. In order to realize a rapid, accurate, robust near-surface seismic imaging, a minimum variance spatial smoothing (MVSS) beamforming method is proposed for the seismic detection and imaging of underground geological structures under a homogeneous assumption. Algorithms such as minimum variance (MV) and spatial smoothing (SS), the coherence factor (CF) matrix, and the diagonal loading (DL) methods were used to improve imaging quality. Furthermore, it was found that a signal advance correction helped improve the focusing effect in near-surface situations. The feasibility and imaging quality of MVSS beamforming are verified in cave models, layer models, and cave-layer models by numerical simulations, confirming that the MVSS beamforming method can be adapted for seismic imaging. The performance of MVSS beamforming is evaluated in the comparison with Kirchhoff migration, the DAS beamforming method, and reverse time migration. MVSS beamforming has a high computational efficiency and a higher imaging resolution. MVSS beamforming also significantly suppresses the unnecessary components in seismic signals such as S-waves, surface waves, and white noise. Moreover, compared with basic delay and sum (DAS) beamforming, MVSS beamforming has a higher vertical resolution and adaptively suppresses interferences. The results show that the MVSS beamforming imaging method might be helpful for detecting near-surface underground structures and for guiding engineering construction.

The Value of CTA Based on Gold Nanorod Contrast Agent in Coronary Artery Diagnosis and Plaque Property Analysis

Coronary CT angiography (CTA) with the characteristics of noninvasive and simple operation is widely used in the diagnosis of coronary artery stenosis. The choice of contrast agent exerts an important impact on the imaging quality of CTA. Conventional iodine contrast agents are easily excreted by the kidneys, from which the imaging window is short, and the imaging quality is poor. Metal nanomaterials have unique optical properties and have broad application prospects in imaging. Our aim is to explore the value of gold nanorod contrast agent in the diagnosis of coronary heart disease. A gold nanorod suspension was first prepared, and the prepared gold nanorod was uniform and had good dispersibility. It can be seen from the light absorption curve that there are two obvious peaks on the UV absorption peak of the gold nanorods. The gold nanorods were cultured in different solutions, and it was found that the particle size of the gold nanorods did not change significantly within 72 hours, indicating that the prepared gold nanorods had good stability. When observing the damage degree of mouse kidney tissue, it was shown that the damage degree of gold nanorod contrast agent to mouse kidney tissue was less than that of iodine contrast agent. The above results indicate that the gold nanorod contrast agent has good stability and safety. Therefore, our study demonstrated that the gold nanorod contrast agent has high value in the diagnosis of coronary arteries and the analysis of plaque properties.