A Comprehensive HPV-STI NGS Assay for Detection of 29 HPV Types and 14 non-HPV Sexually Transmitted Infections

Sexually transmitted infections (STIs) are prevalent throughout the world and impose a significant burden on individual health and public health systems. Missed diagnosis and late treatment of STIs can lead to serious complications such as infertility and cervical cancer. Although sexually transmitted co-infections are common, most commercial assays target one or a few STIs. The HPV-STI ChapterDx Next Generation Sequencing (NGS) assay detects and quantifies 29 HPVs and 14 other STIs in a single-tube and single-step PCR reaction and can be applied to tens to thousands of samples in a single sequencing run. The assay was evaluated in this study, and the limit of detection was 100% at 50 copies for all targets, and 100%, 96%, 88% at 20 copies for 34, 8, and 1 target, respectively. The performance of this assay has been compared to Roche cobas HPV test, showing an overall agreement of 97.5% for hr-HPV, and 98.5% for both, HPV16 and HPV18. The assay also detected all HPV-infected CIN2/3 with 100% agreement with Roche cobas HPV results. Moreover, several co-infections with non-HPV STIs, such as C. trachomatis, T. vaginalis, M. genitalium, and HSV2 were identified. The Chap-terDx HPV-STI NGS assay is a user-friendly, easy to automate and cost-efficient assay, which provides accurate and comprehensive results for a wide spectrum of HPVs and STIs.

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A Polyherbal Formulation for a Wide Spectrum of Reproductive Tract and Sexually Transmitted Infections

Advances in Experimental Medicine and Biology - Complementary and Alternative Approaches to Biomedicine ◽

10.1007/978-1-4757-4820-8_10 ◽

2004 ◽

pp. 111-119 ◽

Cited By ~ 1

Author(s):

G. P. Talwar

Keyword(s):

Sexually Transmitted Infections ◽

Reproductive Tract ◽

Wide Spectrum ◽

Sexually Transmitted ◽

Polyherbal Formulation

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Diagnosis And Antimicrobial Treatment Of Bacterial Of Neisseria Gonorrhea Infections: Update Review Article

The International Arabic Journal of Antimicrobial Agents ◽

10.3823/0803 ◽

2017 ◽

Vol 7 (1) ◽

Author(s):

Asem A. Shehabi ◽

Monzer Hamze

Keyword(s):

Sexually Transmitted Infections ◽

Wide Spectrum ◽

Antimicrobial Treatment ◽

Sexual Contact ◽

Review Article ◽

Arab Countries ◽

World Health ◽

Sexually Transmitted ◽

Neisseria Gonorrhea ◽

Health Organization

Sexually transmitted infections (STIs) are caused by a wide spectrum of bacteria, viruses and parasites. These agents can be easily transmitted during any direct genital or oral sexual contact. Recently, World Health Organization (WHO), reported that more than 1 million STIs are acquired every day worldwide, Each year, there are an estimated 357 million new infections with 1 of 4 STIs: chlamydia, gonorrhoea, syphilis and trichomoniasis. There are few studies and official reports published on the prevalence of STIs in most Arab countries. However, few new recent studies showed increased prevalence of certain STIs in some Arab countries.

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Skin Diseases and Sexually Transmitted Infections among Patients with HIV infection/AIDS referred at the City Institute for Skin and Venereal Diseases in Belgrade: a Case Series of 38 Patients

Serbian Journal of Dermatology and Venerology ◽

10.2478/sjdv-2013-0010 ◽

2013 ◽

Vol 5 (3) ◽

pp. 125-130

Author(s):

Milan Bjekić ◽

Sandra Šipetić

Keyword(s):

Hiv Infection ◽

Sexually Transmitted Infections ◽

Highly Active Antiretroviral Therapy ◽

Skin Diseases ◽

Wide Spectrum ◽

Case Series ◽

Consistent Condom ◽

Venereal Diseases ◽

Sexually Transmitted ◽

The City

Abstract Human immunodeficiency virus (HIV) infection may be associated with a wide spectrum of dermatological disorders. This study reports the prevalence of dermatologic diseases and sexually transmitted infections among 38 HIV-infected adults who consecutively sought treatment at the City Institute for Skin and Venereal Diseases in Belgrade. Patients were referred by their primary HIV providers between January 2011 and June 2012. More than 80% of patients were men who have sex with men. The most prevalent diseases were anogenital warts (36.8%) and syphilis (34.2%), followed by folliculitis and dermatophyte infections (7.9% each). Thirty-four patients (89.5%) were on highly active antiretroviral therapy (HAART) before the first visit to the dermatologist. Although, the pattern of skin disorders was consistent with literature data, high prevalence of sexually transmitted infections among our patients was rather peculiar. These facts point to the need for education of sexually active persons with HIV about the consequences of sexually transmitted infections, risks of transmission of drug resistant HIV strains, as well as safe sex practice and consistent condom use.

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