scholarly journals Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophages

2021 ◽  
Author(s):  
Joshua D'Rozario ◽  
Konstantin Knoblich ◽  
Mechthild Luetge ◽  
Christian Perez Shibayama ◽  
Hung-Wei Cheng ◽  
...  
Keyword(s):  
Lymph Node ◽  
Key Factors ◽  
Rapid Loss ◽  
Major Site ◽  
Genetic Ablation ◽  
Functional Assays ◽  
Single Cell Rna Sequencing ◽  
Reticular Cells ◽  
Macrophage Populations ◽  
Fibroblastic Reticular Cells

The lymph node (LN) is home to resident macrophage populations that are essential for immune function and homeostasis. The T cell paracortical zone is a major site of macrophage efferocytosis of apoptotic cells, but key factors controlling this niche are undefined. Here we show that fibroblastic reticular cells (FRCs) are an essential component of the LN macrophage niche. Macrophages co-localised with FRCs in human LNs, and murine single-cell RNA-sequencing revealed that most reticular cells expressed master macrophage regulator CSF1. Functional assays showed that CSF1R signalling was sufficient to support macrophage development. In the presence of LPS, FRCs underwent a mechanistic switch and maintained support through CSF1R-independent mechanisms. These effects were conserved between mouse and human systems. Rapid loss of macrophages and monocytes from LNs was observed upon genetic ablation of FRCs. These data reveal a critically important role for FRCs in the creation of the parenchymal macrophage niche within LNs.

scholarly journals Activation of fibroblastic reticular cells in kidney lymph node during crescentic glomerulonephritis

2019 ◽  
Vol 95 (2) ◽  
pp. 310-320 ◽  
Author(s):  
Vivek Kasinath ◽  
Osman A. Yilmam ◽  
Mayuko Uehara ◽  
Liwei Jiang ◽  
Farideh Ordikhani ◽  
...  
Keyword(s):  
Lymph Node ◽  
Crescentic Glomerulonephritis ◽  
Reticular Cells ◽  
Fibroblastic Reticular Cells

scholarly journals Tissue-Engineered Stromal Reticula to Study Lymph Node Fibroblastic Reticular Cells in Type I Diabetes

2020 ◽  
Vol 13 (5) ◽  
pp. 419-434
Author(s):  
Freddy Gonzalez Badillo ◽  
Flavia Zisi Tegou ◽  
Riccardo Masina ◽  
Shane Wright ◽  
Mackenzie Scully ◽  
...  
Keyword(s):  
Lymph Node ◽  
Type I Diabetes ◽  
Type I ◽  
Reticular Cells ◽  
Fibroblastic Reticular Cells

scholarly journals Murine Fibroblastic Reticular Cells From Lymph Node Interact With CD4+ T Cells Through CD40-CD40L

2015 ◽  
Vol 99 (8) ◽  
pp. 1561-1567 ◽  
Author(s):  
Yumi Nakayama ◽  
C. Colin Brinkman ◽  
Jonathan S. Bromberg
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Cd4 T Cells ◽  
Reticular Cells ◽  
Fibroblastic Reticular Cells

scholarly journals Lymph node fibroblastic reticular cells steer immune responses

2021 ◽  
Author(s):  
Lushen Li ◽  
Jing Wu ◽  
Reza Abdi ◽  
Christopher M. Jewell ◽  
Jonathan S. Bromberg
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Reticular Cells ◽  
Fibroblastic Reticular Cells

scholarly journals Lymph Node Fibroblastic Reticular Cells Construct the Stromal Reticulum via Contact with Lymphocytes

2004 ◽  
Vol 200 (6) ◽  
pp. 783-795 ◽  
Author(s):  
Tomoya Katakai ◽  
Takahiro Hara ◽  
Manabu Sugai ◽  
Hiroyuki Gonda ◽  
Akira Shimizu
Keyword(s):  
Extracellular Matrix ◽  
Lymph Node ◽  
Lymphocyte Activation ◽  
Nuclear Factor Κb ◽  
Dependent Manner ◽  
Reticular Cells ◽  
Β Receptor ◽  
Factor Α ◽  
Lymphotoxin Α ◽  
Fibroblastic Reticular Cells

The sophisticated microarchitecture of the lymph node, which is largely supported by a reticular network of fibroblastic reticular cells (FRCs) and extracellular matrix, is essential for immune function. How FRCs form the elaborate network and remodel it in response to lymphocyte activation is not understood. In this work, we established ERTR7+gp38+VCAM-1+ FRC lines and examined the production of the ER-TR7 antigen. Multiple chemokines produced by FRCs induced T cell and dendritic cell chemotaxis and adhesion to the FRC surface. FRCs can secrete the ER-TR7 antigen as an extracellular matrix component to make a reticular meshwork in response to contact with lymphocytes. The formation of the meshwork is induced by stimulation with tumor necrosis factor-α or lymphotoxin-α in combination with agonistic antibody to lymphotoxin-β receptor in a nuclear factor-κB (RelA)–dependent manner. These findings suggest that signals from lymphocytes induce FRCs to form the network that supports the movement and interactions of immune effectors within the lymph node.

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