The heterochromatin landscape in migrating cells and the importance of H3K27me3 for migration-associated transcriptional changes

SummaryH3K9me3, H3K27me3 and H4K20me1 are epigenetic markers associated with chromatin condensation and transcriptional repression. Previously, we found that migration of melanoma cells is associated with and dependent on global chromatin condensation that includes a global increase in these markers. Taken together with more recent reports by others suggest it is a general signature of migrating cells. Here, to learn about the function of these markers in migrating cells we mapped them by ChIP-seq analysis. This analysis revealed that induction of migration leads to expansion of these markers along the genome and to an increased overlapping between them. Significantly, induction of migration led to a higher increase in H3K9me3 and H4K20me1 signals at repetitive elements than at protein-coding genes, while an opposite pattern was found for H3K27me3. Transcriptome analysis revealed that 182 altered genes following induction of migration, of which 33% are dependent on H3K27me3 for these changes. H3K27me3 was also required to prevent changes in the expression of 501 other genes upon induction of migration. Taken together our results suggest that heterochromatinization in migrating cells is global and not restricted to specific genomic loci and that H3K27me3 is a key component in executing a migration-specific transcriptional plan.

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The Heterochromatin Landscape in Migrating Cells and the Importance of H3K27me3 for Associated Transcriptome Alterations

Cells ◽

10.3390/cells7110205 ◽

2018 ◽

Vol 7 (11) ◽

pp. 205 ◽

Cited By ~ 8

Author(s):

Tamar Segal ◽

Mali Salmon-Divon ◽

Gabi Gerlitz

Keyword(s):

Transcriptome Analysis ◽

Transcriptional Repression ◽

Chromatin Condensation ◽

Opposite Pattern ◽

Protein Coding ◽

Epigenetic Markers ◽

Protein Coding Genes ◽

Global Increase ◽

General Signature ◽

Migrating Cells

H3K9me3, H3K27me3, and H4K20me1 are epigenetic markers associated with chromatin condensation and transcriptional repression. Previously, we found that migration of melanoma cells is associated with and dependent on global chromatin condensation that includes a global increase in these markers. Taken together with more recent reports by others suggests it is a general signature of migrating cells. Here, to learn about the function of these markers in migrating cells, we mapped them by ChIP-seq analysis. This analysis revealed that induction of migration leads to expansion of these markers along the genome and to an increased overlapping between them. Significantly, induction of migration led to a higher increase in H3K9me3 and H4K20me1 signals at repetitive elements than at protein-coding genes, while an opposite pattern was found for H3K27me3. Transcriptome analysis revealed 182 altered genes following induction of migration, of which 33% are dependent on H3K27me3 for these changes. H3K27me3 was also required to prevent changes in the expression of 501 other genes upon induction of migration. Taken together, our results suggest that heterochromatinization in migrating cells is global and not restricted to specific genomic loci and that H3K27me3 is a key component in executing a migration-specific transcriptional plan.

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Long non-coding RNA discovery in Anopheles gambiae using deep RNA sequencing

10.1101/007484 ◽

2014 ◽

Cited By ~ 1

Author(s):

Adam M Jenkins ◽

Robert M Waterhouse ◽

Alan S Kopin ◽

Marc A.T. Muskavitch

Keyword(s):

Anopheles Gambiae ◽

Rna Sequencing ◽

Transcriptional Repression ◽

Expression Profiles ◽

Sequence Evolution ◽

Life Stages ◽

Protein Coding ◽

Malaria Burden ◽

Protein Coding Genes ◽

Non Coding Rnas

Long non-coding RNAs (lncRNAs) are mRNA-like transcripts longer than 200 bp that have no protein-coding potential. lncRNAs have recently been implicated in epigenetic regulation, transcriptional and post-transcriptional gene regulation, and regulation of genomic stability in mammals, Caenorhabditis elegans, and Drosophila melanogaster. Using deep RNA sequencing of multiple Anopheles gambiae life stages, we have identified over 600 novel lncRNAs and more than 200 previously unannotated putative protein-coding genes. The lncRNAs exhibit differential expression profiles across life stages and adult genders. Those lncRNAs that are antisense to known protein-coding genes or are contained within intronic regions of protein-coding genes may mediate transcriptional repression or stabilization of associated mRNAs. lncRNAs exhibit faster rates of sequence evolution across anophelines compared to previously known and newly identified protein-coding genes. This initial description of lncRNAs in An. gambiae offers the first genome-wide insights into long non-coding RNAs in this vector mosquito and defines a novel set of potential targets for the development of vector-based interventions that may curb the human malaria burden in disease-endemic countries.

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