scholarly journals Age and genetic background determine hybrid male sterility in house mice

2018 ◽  
Author(s):  
Samuel J. Widmayer ◽  
David L. Aylor
Keyword(s):  
Reproductive Isolation ◽  
Male Sterility ◽  
Genetic Factors ◽  
Inbred Strains ◽  
House Mice ◽  
Reproductive Barriers ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Unique Type ◽  
Age Dependent

AbstractHybrid male sterility (HMS) is a unique type of reproductive isolation commonly observed between house mouse (Mus musculus) subspecies in the wild and in laboratory crosses. We identified hybrids that display three distinct trajectories of fertility despite having identical genotypes at the major HMS gene Prdm9 and the X Chromosome. In each case, we crossed female PWK/PhJ mice representative of the M.m.musculus subspecies to males from classical inbred strains representative of M.m.domesticus: 129S1/SvImJ, A/J, C57BL/6J, and DBA/2J. PWK129S1 males are always sterile, while PWKDBA2 males escape HMS. In addition, we observe age-dependent sterility in PWKB6 and PWKAJ males. These males are fertile between 15 and 35 weeks with moderate penetrance. These results point to multiple segregating HMS modifier alleles, some of which have an age-dependent mode of action. Age-dependent mechanisms could have broad implications for the maintenance of reproductive barriers in nature.Author SummaryTwo subspecies of house mice show partial reproductive barriers in nature, and may be in the process of speciation. We used mice derived from each subspecies to replicate hybrid male sterility (HMS) in laboratory mice. Two major genetic factors are well established as playing a role in mouse HMS, but the number of additional factors and their mechanisms are unknown. We characterized reproductive trait variation in a set of hybrid male mice that were specifically designed to eliminate the effects of known genetic factors. We discovered that age played an important role in fertility of some hybrids. These hybrid males showed a delayed onset of fertility, then became fertile for only a few weeks. Across all hybrids males in our study, we observed three distinct trajectories of fertility: complete fertility, complete sterility, and age-dependent fertility. These results point to two or more critical HMS variants with large enough effects to completely restore fertility. This study advances our understanding of the genetic architecture and biological mechanisms of reproductive isolation in mice.

scholarly journals Age and genetic background modify hybrid male sterility in house mice

2020 ◽  
Author(s):  
Samuel J. Widmayer ◽  
Mary Ann Handel ◽  
David L. Aylor
Keyword(s):  
Male Sterility ◽  
House Mouse ◽  
Genetic Background ◽  
Genetic Architecture ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
X Chromosomes ◽  
Age Related ◽  
Age Dependent ◽  
In The Wild

AbstractHybrid male sterility (HMS) contributes to reproductive isolation commonly observed among house mouse (Mus musculus) subspecies, both in the wild and in laboratory crosses. Incompatibilities involving specific Prdm9 alleles and certain Chromosome (Chr) X genotypes are known determinants of fertility and HMS, and previous work in the field has demonstrated that genetic background modifies these two major loci. We constructed hybrids that have identical genotypes at Prdm9 and identical X chromosomes, but differ widely across the rest of the genome. In each case, we crossed female PWK/PhJ mice representative of the M. m. musculus subspecies to males from a classical inbred strain representative of M. m. domesticus: 129S1/SvImJ, A/J, C57BL/6J, or DBA/2J. We detected three distinct trajectories of fertility among the hybrids using breeding experiments. The PWK129S1 males were always infertile. PWKDBA2 males were fertile, despite their genotypes at the major HMS loci. We also observed age-dependent changes in fertility parameters across multiple genetic backgrounds. The PWKB6 and PWKAJ males were always infertile before 15 weeks and after 35 weeks, yet some PWKB6 and PWKAJ males were fertile between fifteen and 35 weeks. This observation could resolve previous contradictory reports about the fertility of PWKB6. Taken together, these results point to multiple segregating HMS modifier alleles, some of which have age-related modes of action. The ultimate identification of these alleles and their age-related mechanisms will advance understanding both of the genetic architecture of HMS and of how reproductive barriers are maintained between house mouse subspecies.

scholarly journals Extensive introgression despite Haldane’s rule: insights from grasshopper hybrid zones

2021 ◽  
Author(s):  
Linda Hagberg ◽  
Enrique Celemin ◽  
Iker Irisarri ◽  
Oliver Hawlitschek ◽  
J L Bella ◽  
...  
Keyword(s):  
Gene Flow ◽  
Reproductive Isolation ◽  
Male Sterility ◽  
Secondary Contact ◽  
Hybrid Zones ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Haldane’S Rule ◽  
Haldane's Rule ◽  
Species Formation

Although the process of species formation is notoriously idiosyncratic, the observation of pervasive patterns of reproductive isolation across species pairs suggests that generalities, or “rules”, underlie species formation in all animals. Haldane’s rule states that whenever a sex is absent, rare or sterile in a cross between two taxa, that sex is usually the heterogametic sex. Yet, understanding how Haldane’s rule first evolves and whether it is associated to genome wide barriers to gene flow remains a challenging task because this rule is usually studied in highly divergent taxa that no longer hybridize in nature. Here, we address these questions using the meadow grasshopper Pseudochorthippus parallelus where populations that readily hybridize in two natural hybrid zones show hybrid male sterility in laboratorial crosses. Using mitochondrial data, we infer that such populations have diverged some 100,000 years ago, surviving multiple glacial periods in isolated Pleistocenic refugia. Nuclear data shows that secondary contact has led to extensive introgression throughout the species range, including between populations showing hybrid male sterility. We find repeatable patterns of genomic differentiation across the two hybrid zones, yet such patterns are consistent with shared genomic constraints across taxa rather than their role in reproductive isolation. Together, our results suggest that Haldane’s rule can evolve relatively quickly within species, particularly when associated to strong demographic changes. At such early stages of species formation, hybrid male sterility still permits extensive gene flow, allowing future studies to identify genomic regions associated with reproductive barriers.

scholarly journals The Contribution of the Y Chromosome to Hybrid Male Sterility in House Mice

Genetics ◽  
2012 ◽  
Vol 191 (4) ◽  
pp. 1271-1281 ◽  
Author(s):  
Polly Campbell ◽  
Jeffrey M. Good ◽  
Matthew D. Dean ◽  
Priscilla K. Tucker ◽  
Michael W. Nachman
Keyword(s):  
Male Sterility ◽  
Y Chromosome ◽  
House Mice ◽  
Hybrid Male ◽  
Hybrid Male Sterility

scholarly journals ASYMMETRY AND POLYMORPHISM OF HYBRID MALE STERILITY DURING THE EARLY STAGES OF SPECIATION IN HOUSE MICE

Evolution ◽  
2007 ◽  
Vol 0 (0) ◽  
pp. 071115145922007-??? ◽  
Author(s):  
Jeffrey M. Good ◽  
Mary Ann Handel ◽  
Michael W. Nachman
Keyword(s):  
Male Sterility ◽  
House Mice ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Early Stages

scholarly journals Evaluation of the courtship and of the hybrid male sterility among Drosophila buzzatii cluster species (Diptera, Drosophilidae)

2002 ◽  
Vol 62 (4a) ◽  
pp. 601-608 ◽  
Author(s):  
L. P. de B. MACHADO ◽  
J. P.de CASTRO ◽  
L. MADI-RAVAZZI
Keyword(s):  
Reproductive Isolation ◽  
Male Sterility ◽  
F1 Hybrids ◽  
Interspecific Crosses ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Drosophila Buzzatii ◽  
Repleta Group ◽  
Reproductive Isolation Mechanisms ◽  
Isolation Mechanisms

In the Drosophila repleta group the establishment of subgroups and complexes made on the basis of morphological and cytological evidences is supported by tests of reproductive isolation. Among species in the repleta group, the buzzatii cluster, due to its polymorphism and polytipism, is an excellent material for ecological and speciation studies. Some interspecific crosses involving Drosophila seriema, Drosophila sp. B, D. koepferae and D. buzzatii strains were completely sterile while others involving strains from these species produced F1 hybrids that did not yield F2. In the present work, data on courtship duration and copula occurrence obtained in the analysis of flies from parental sterile crosses and on spermatozoon mobility observed in F1 hybrids that did not yield F2 are presented. Copula did not occur during one hour of observation and the spermatozoon also did not show mobility at any of the analyzed stages (3, 7, 9 and 10 days old). There was a high variation in courtship average duration and in the percentage of males that courted the females. The reproductive isolation mechanisms indicated by these observations were pre and post-zygotic, as supported by the absence of copula and male sterility. Data obtained also showed the occurrence of different degrees of reproductive compatibility among the strains classified as the same species but from distinct geographic localities.

scholarly journals The Genetic Basis of Postzygotic Reproductive Isolation Between Drosophila santomea and D. yakuba Due to Hybrid Male Sterility

Genetics ◽  
2006 ◽  
Vol 173 (1) ◽  
pp. 225-233 ◽  
Author(s):  
Amanda J. Moehring ◽  
Ana Llopart ◽  
Susannah Elwyn ◽  
Jerry A. Coyne ◽  
Trudy F. C. Mackay
Keyword(s):  
Reproductive Isolation ◽  
Male Sterility ◽  
Genetic Basis ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Postzygotic Reproductive Isolation

scholarly journals Genetics and Evolution of Hybrid Male Sterility in House Mice

Genetics ◽  
2012 ◽  
Vol 191 (3) ◽  
pp. 917-934 ◽  
Author(s):  
Michael A. White ◽  
Maria Stubbings ◽  
Beth L. Dumont ◽  
Bret A. Payseur
Keyword(s):  
Male Sterility ◽  
House Mice ◽  
Hybrid Male ◽  
Hybrid Male Sterility

scholarly journals Age and Genetic Background Modify Hybrid Male Sterility in House Mice

Genetics ◽  
2020 ◽  
Vol 216 (2) ◽  
pp. 585-597 ◽  
Author(s):  
Samuel J. Widmayer ◽  
Mary Ann Handel ◽  
David L. Aylor
Keyword(s):  
Male Sterility ◽  
House Mouse ◽  
Genetic Background ◽  
Genetic Architecture ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
X Chromosomes ◽  
Age Related ◽  
Age Dependent ◽  
In The Wild

Hybrid male sterility (HMS) contributes to reproductive isolation commonly observed among house mouse (Mus musculus) subspecies, both in the wild and in laboratory crosses. Incompatibilities involving specific Prdm9 alleles and certain Chromosome (Chr) X genotypes are known determinants of fertility and HMS, and previous work in the field has demonstrated that genetic background modifies these two major loci. We constructed hybrids that have identical genotypes at Prdm9 and identical X chromosomes, but differ widely across the rest of the genome. In each case, we crossed female PWK/PhJ mice representative of the M. m. musculus subspecies to males from a classical inbred strain representative of M. m. domesticus: 129S1/SvImJ, A/J, C57BL/6J, or DBA/2J. We detected three distinct trajectories of fertility among the hybrids using breeding experiments. The PWK129S1 males were always infertile. PWKDBA2 males were fertile, despite their genotypes at the major HMS loci. We also observed age-dependent changes in fertility parameters across multiple genetic backgrounds. The PWKB6 and PWKAJ males were always infertile before 12 weeks and after 35 weeks. However, some PWKB6 and PWKAJ males were transiently fertile between 12 and 35 weeks. This observation could resolve previous contradictory reports about the fertility of PWKB6. Taken together, these results point to multiple segregating HMS modifier alleles, some of which have age-related modes of action. The ultimate identification of these alleles and their age-related mechanisms will advance understanding both of the genetic architecture of HMS and of how reproductive barriers are maintained between house mouse subspecies.

scholarly journals A Complex Genetic Basis to X-Linked Hybrid Male Sterility Between Two Species of House Mice

Genetics ◽  
2008 ◽  
Vol 179 (4) ◽  
pp. 2213-2228 ◽  
Author(s):  
Jeffrey M. Good ◽  
Matthew D. Dean ◽  
Michael W. Nachman
Keyword(s):  
Male Sterility ◽  
Genetic Basis ◽  
House Mice ◽  
Hybrid Male ◽  
Hybrid Male Sterility
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