Reciprocal intercompatibility between Byrsonima Rich. exKunth species (Malpighiaceae), in an urban ecotonal fragment of Semi-Deciduous Seasonal Forest and Cerrado

Byrsonima species present floral monomorphism and sharing visitor/pollinator guild. These traits suggest the hypothesis evaluation of reciprocal inter-incompatibility between two syntopic Byrsonima species: B. pachyphylla and B. crassifolia. Pollen tube growth with similar behavior was observed in the stigmatic surface, pistil canal, ovary and micropylar channel to both species. In addition, partial self-incompatibility in self-pollination with greater fruiting in autogamy was observed. Cross-pollination and self-pollination coexist, and reciprocal intercompatibility occurs. Prezygotic isolation mechanisms are unlikely by the absence of abnormal pollen tubes, higher fruiting production and absence of hybrids in the study site. Las especies de Byrsonima presentan monomorfismo floral y comparten gremio de visitantes/polinizadores. Estos rasgos sugieren la evaluación de hipótesis de interincompatibilidad recíproca entre dos especies sintópicas de Byrsonima: B. pachyphylla y B. crassifolia. Se observó el crecimiento del tubo polínico con comportamiento similar en la superficie estigmática, canal del pistilo, ovario y canal micropilar de ambas especies. Además, se describió la autoincompatibilidad parcial en la autopolinización con mayor fructificación en la autogamia. La polinización cruzada y la autopolinización coexisten y se produce una intercompatibilidad recíproca. Los mecanismos de aislamiento precigóticos son improbables por la ausencia de tubos polínicos anormales, producción de frutos y ausencia de híbridos en la área de estudio.

Securing Interruptible Enclaved Execution on Small Microprocessors

Computer systems often provide hardware support for isolation mechanisms such as privilege levels, virtual memory, or enclaved execution. Over the past years, several successful software-based side-channel attacks have been developed that break, or at least significantly weaken, the isolation that these mechanisms offer. Extending a processor with new architectural or micro-architectural features brings a risk of introducing new software-based side-channel attacks. This article studies the problem of extending a processor with new features without weakening the security of the isolation mechanisms that the processor offers. Our solution is heavily based on techniques from research on programming languages. More specifically, we propose to use the programming language concept of full abstraction as a general formal criterion for the security of a processor extension. We instantiate the proposed criterion to the concrete case of extending a microprocessor that supports enclaved execution with secure interruptibility. This is a very relevant instantiation, as several recent papers have shown that interruptibility of enclaves leads to a variety of software-based side-channel attacks. We propose a design for interruptible enclaves and prove that it satisfies our security criterion. We also implement the design on an open-source enclave-enabled microprocessor and evaluate the cost of our design in terms of performance and hardware size.

Breaking TrustZone memory isolation and secure boot through malicious hardware on a modern FPGA-SoC

FPGA-SoCs are heterogeneous embedded computing platforms consisting of reconfigurable hardware and high-performance processing units. This combination offers flexibility and good performance for the design of embedded systems. However, allowing the sharing of resources between an FPGA and an embedded CPU enables possible attacks from one system on the other. This work demonstrates that a malicious hardware block contained inside the reconfigurable logic can manipulate the memory and peripherals of the CPU. Previous works have already considered direct memory access attacks from malicious logic on platforms containing no memory isolation mechanism. In this work, such attacks are investigated on a modern platform which contains state-of-the-art memory and peripherals isolation mechanisms. We demonstrate two attacks capable of compromising a Trusted Execution Environment based on ARM TrustZone and show a new attack capable of bypassing the secure boot configuration set by a device owner via the manipulation of Battery-Backed RAM and eFuses from malicious logic.

Bio-Inspired Vibration Isolation: Methodology and Design

Various bio-inspired vibration isolators have been emerged in recent decades and applied successfully in the protection of sensitive components, improvement of operating comfort, enhancement of control accuracy, etc. They are generally developed by exploiting favorable nonlinearities in biological structures. The main contribution of this work is to provide a comprehensive review of recent studies on the bio-inspired isolators. The methodology of bio-inspired vibration isolation is proposed from the perspective of mechanics based on the elemental theory and design principles. The key isolation mechanisms are classified into three categories according to different dominant forces: stiffness adjustment mechanism, auxiliary mass mechanism, and damping mechanism, respectively. Some representative designs, performance analyses, and practical applications of each type of bio-inspired isolators are also provided. In bio-inspired isolators with variable stiffness, the inherent structural performances can be adjusted to deal with variation in external load. The auxiliary mass mechanism utilizes nonlinear inertial effects to achieve ultralow frequency vibration isolation. Unique damping mechanism of bio-inspired structures is often studied to protect devices and equipment from impact loads. Bio-inspired vibration methods can also be applied in active/semi-active control systems with advantages of low energy consumption and high robustness. Finally, the review ends with conclusions, which highlight resolved and unresolved issues and provide a brief outlook on future perspectives. This review aims to give a comprehensive understanding of bio-inspired isolation mechanism. It also provides guidance on designing new bio-inspired isolators for improving their vibration isolation performance.

Length-weight relationships Silver barb (Barbonymus gonionotus) in Mamberamo River, Papua

There are big rivers in Papua province, one of which is the Mamberamo River. The Mamberamo River, known as the Amazon of Indonesia, has very high biodiversity. Silver barb is one of the fish caught in the Mamberamo River. Silver barb is an adaptable fish, has many types and varies. Variations in Silver barb are caused by interactions with the environment as well as isolation mechanisms. This research explains the relationship between weight-length and condition factors Silver barb in Mamberamo River, Papua. The research location consists of four observation stations: Kali Merah, Kerumi, Telaga, and Sungai Putus. Sampling was carried out in February, May, August, and October 2016. The research method used was a survey using an enumerator and analysis using the LAM (linear allometric) model. The results showed that Silver barb growth was allometric negative in February and October, but in May and August, growth was allometric positive. The factor conditions for the Silver barb range 1.014-1.02.

Defining and Disrupting Species Boundaries in Saccharomyces

The genus Saccharomyces is an evolutionary paradox. On the one hand, it is composed of at least eight clearly phylogenetically delineated species; these species are reproductively isolated from each other, and hybrids usually cannot complete their sexual life cycles. On the other hand, Saccharomyces species have a long evolutionary history of hybridization, which has phenotypic consequences for adaptation and domestication. A variety of cellular, ecological, and evolutionary mechanisms are responsible for this partial reproductive isolation among Saccharomyces species. These mechanisms have caused the evolution of diverse Saccharomyces species and hybrids, which occupy a variety of wild and domesticated habitats. In this article, we introduce readers to the mechanisms isolating Saccharomyces species, the circumstances in which reproductive isolation mechanisms are effective and ineffective, and the evolutionary consequences of partial reproductive isolation. We discuss both the evolutionary history of the genus Saccharomyces and the human history of taxonomists and biologists struggling with species concepts in this fascinating genus.

Self Diagnostics and Isolation Mechanisms for Mixed Criticality Systems

Virtualization is a technology that is frequently employed in computers and servers to provide isolation for execution environments, and to support the execution of multiple Operating Systems (OS) on the same hardware platform. In the embedded systems´ world, virtualization has been a rising trend, essentially because it offers an isolation mechanism that provides hardware manufacturer´ independence and it avoids obsolescence issues. The isolation mechanism supports safety and security measures, and assists in the certification of safety-critical systems. Virtualization offers improved performances, better transparency, portability and interoperability by integrating hardware and software resources, and also networking services into one computing entity. It makes the integration process of Mixed Criticality Systems (MCS) easier. For industries, FieldProgrammable Gate Arrays (FPGAs) hardware solutions provide the needed level of flexibility and performance. In this paper, a Self-test application is integrated in the hardware and also in the software level. The importance of self-test applications for Instrumentation and Control (I&C) systems will be discussed in the context of virtualization. For this implementation a type 1 hypervisor called Xtratum is used. An analysis of inter-partition communication channels´ performance will be provided including the implications multicore approaches will have on communication. The novelty of this work is to study the isolation impact multicore approaches can have on inter-partitions communications in Xtratum. Another novel aspect is the implementation of a self-test application in the hypervisor and the board as well.

Transcriptomic Analysis Reveals Insect Hormone Biosynthesis Pathway Involved in Desynchronized Development Phenomenon in Hybridized Sibling Species of Tea Geometrids (Ectropis grisescens and Ectropis obliqua)

Ectropis grisescens and Ectropis obliqua are sibling species of tea-chewing pests. An investigation of the distribution of tea geometrids was implemented for enhancing controlling efficiency. E. grisescens is distributed across a wider range of tea-producing areas than Ectropis obliqua in China with sympatric distribution found in some areas. In order to explore reproductive isolation mechanisms in co-occurrence areas, hybridization experiments were carried out. Results showed they can mate but produce infertile hybrids. During experiments, the desynchronized development phenomenon was found in the hybridized generation of sibling tea geometrids. Furthermore, transcriptome analysis of those individuals of fast-growing and slow-growing morphs revealed that the insect hormone biosynthesis pathway was enriched in two unsynchronized development groups of hybrid offspring. More importantly, some genes regulating the synthesis of moulting hormone showed significantly up-regulated expression in fast-growing groups. Above all, metabolism of the juvenile hormone and synthesis of the ecdysone pathway were found to be crucially involved in the desynchronized development phenomenon. This research finding contributes to a better understanding of the mechanisms of insect development and reproductive isolation of two sibling species.

Impact of hybridisation in two Cossypha robin-chat species in southern Africa

Chorister Robin-Chat Cossypha dichroa, a South African forest endemic, and Red-capped Robin-Chat C. natalensis, a widely distributed species in African forest and woodland, are inferred to hybridise in areas of sympatry. DNA was extracted from blood samples of C. dichroa (n = 18), C. natalensis (n = 47), and two phenotypic hybrids. The mitochondrial cytochrome c oxidase I (COI) gene was amplified by PCR and sequenced. Phylogenetic analysis was performed on the sequence data to investigate taxonomic status and putative interspecific hybridisation. Phenotypic hybrids grouped with C. natalensis, suggesting maternal parentage from that species. Intra- and interspecific genetic and geographic distances were compared between C. dichroa and C. natalensis to assess genetic introgression. Seven of the thirteen microsatellite primer pairs developed for C. natalensis cross amplified in C. dichroa. These seven markers were then used for further analysis. STRUCTURE v2.3.4 was used to assign individuals to a particular genetic cluster and determine any admixture. NEWHYBRIDS v1.1 was used to assign hybrid status to samples beyond the F1 generation. Despite the hybridisation events recorded between C. dichroa and C. natalensis they still form two separate clusters as expected, and two genetic clusters (K=2) were identified using STRUCTURE. These two species are proficient vocal mimics and it is likely that reproductive isolation mechanisms are overcome through vocalisations. Genotypic hybrids are evident in the sampled population and hybridisation and backcrossing across a zone of sympatry is occurring. However, hybridisation is expected to have very little evolutionary influence on the integrity of recently diverged species which retain reproductive isolation across a wide region of sympatry through call distinctness.

Impact of hybridisation in two Cossypha robin-chat species in southern Africa

Chorister Robin-Chat Cossypha dichroa, a South African forest endemic, and Red-capped Robin-Chat C. natalensis, a widely distributed species in African forest and woodland, are inferred to hybridise in areas of sympatry. DNA was extracted from blood samples of C. dichroa (n = 18), C. natalensis (n = 47), and two phenotypic hybrids. The mitochondrial cytochrome c oxidase I (COI) gene was amplified by PCR and sequenced. Phylogenetic analysis was performed on the sequence data to investigate taxonomic status and putative interspecific hybridisation. Phenotypic hybrids grouped with C. natalensis, suggesting maternal parentage from that species. Intra- and interspecific genetic and geographic distances were compared between C. dichroa and C. natalensis to assess genetic introgression. Seven of the thirteen microsatellite primer pairs developed for C. natalensis cross amplified in C. dichroa. These seven markers were then used for further analysis. STRUCTURE v2.3.4 was used to assign individuals to a particular genetic cluster and determine any admixture. NEWHYBRIDS v1.1 was used to assign hybrid status to samples beyond the F1 generation. Despite the hybridisation events recorded between C. dichroa and C. natalensis they still form two separate clusters as expected, and two genetic clusters (K=2) were identified using STRUCTURE. These two species are proficient vocal mimics and it is likely that reproductive isolation mechanisms are overcome through vocalisations. Genotypic hybrids are evident in the sampled population and hybridisation and backcrossing across a zone of sympatry is occurring. However, hybridisation is expected to have very little evolutionary influence on the integrity of recently diverged species which retain reproductive isolation across a wide region of sympatry through call distinctness.