scholarly journals Modelling the in vitro pre-infection dynamics of Colletotrichum spp. isolated from yellow passion fruit in function of environmental variables

2019 ◽  
Author(s):  
Karinna Vieira Chiacchio Velame ◽  
Hermes Peixoto Santos Filho ◽  
Adelise de Almeida Lima ◽  
Carlos Augusto Dórea Bragança ◽  
Francisco Ferraz Laranjeira
Keyword(s):  
Environmental Variables ◽  
Climatic Conditions ◽  
Passion Fruit ◽  
Yield Reduction ◽  
Post Harvest ◽  
Infection Dynamics ◽  
Mycelia Growth ◽  
Wetness Period ◽  
Yellow Passion Fruit

AbstractBrazil is the largest world producer of yellow passion fruit, but the mean yield (14.3t.ha-1) is less than half the potential of the crop. Part of this difference can be explained by plant health problems, including anthracnose caused by Colletotrichum spp. In regions with favorable climatic conditions, anthracnose can be a factor of significant yield reduction, but these regions have not yet been zoned. The objective of this study was to model the pre-infection dynamics of the fungus. The influence of temperature and photoperiod was studied on mycelia growth, sporulation and conidia germination. Mathematical models were fitted to the results and the optima for the environmental variables were estimated. The maximum mycelia growth was estimated to occur at 26.5°C. Between 24.5°C and 28.5°C the fungus grew from 95% to 100% of the estimated maximum. Temperatures below 13°C or above 34°C were harmful to mycelia growth. Temperatures over 26°C were the most favorable to sporulation while below 13°C sporulation was only 5% of the maximum. Optimum germination occurred between 25°C and 29°C with the ideal wetness period between 11h and 13h. These results can be used as a basis for zoning the risk of anthracnose occurrence in passion fruit producing regions.Significance and Impact of the StudyMany diseases affect the yellow passion fruit crop, limiting its yield; among them anthracnose, caused by Colletotrichum spp. The disease occurs in both field (leaf and stem symptoms) and post-harvest (fruits) conditions. Understanding the role environmental conditions play in the biological cycle of such diseases is essential for developing management strategies. By modelling mycelial growth, spore production and spore germination of Colletotrichum spp. as affected by temperature, photoperiod and wetness period, it was possible to characterize the pathogen’s pre-infectional dynamics. The results should be used as a first approximation to estimate the risk of anthracnose occurrence in pre- or post-harvest.

scholarly journals Evaluation of different methods to overcome in vitro seed dormancy from yellow passion fruit

2014 ◽  
Vol 13 (36) ◽  
pp. 3657-3665 ◽  
Author(s):  
M. M. Rego, ◽  
E. R. Rego, ◽  
L. P. U. Nattrodt, ◽  
P. A. Barroso, ◽  
F. L. Finger, ◽  
...  
Keyword(s):  
Seed Dormancy ◽  
Passion Fruit ◽  
Yellow Passion Fruit

scholarly journals Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

2020 ◽  
Vol 42 ◽  
pp. e48321
Author(s):  
João Arthur dos Santos Oliveira ◽  
Andressa Domingos Polli ◽  
Julio Cesar Polonio ◽  
Ravely Casarotti Orlandelli ◽  
Hélio Conte ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Growth Promotion ◽  
Passion Fruit ◽  
Agricultural Field ◽  
Bean Plants ◽  
Causative Agents ◽  
Colonization Frequency ◽  
Greenhouse Assay ◽  
Yellow Passion Fruit

Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried out greenhouse experiments in bean seedlings. A high colonization frequency was obtained (89%), and the molecular identification based on DNA sequencing attested Colletotrichum as the most frequent genus and minor occurrence of Curvularia endophytes. The endophytes tested showed different types of competitive interactions in in vitro antagonism inhibition rate ranging from 28.8 to 48.8%. There were 10 promising antagonists tested for their antagonist activity of crude extracts of secondary metabolites, in which strain PE-36 (20.8%) stood out among the other strains evaluated. In the greenhouse assay, plants inoculated only with endophyte Colletotrichum sp. PE-36 was symptomless and suggest that the endophyte strengthened the growth promotion in common bean plants, especially in the root length and number of leaves when compared to control plants and other treatments. Despite many fungi of Colletotrichum genus being described as causative agents of anthracnose, in this study, the plant sampled was colonized predominantly by Colletotrichum endophytes living in asymptomatic relationship. By the way, we come across a Colletotrichum sp. endophyte able to antagonize a Colletotrichum sp. pathogen.

scholarly journals Resistance to Passion fruit woodiness virus in Transgenic Passionflower Expressing the Virus Coat Protein Gene

Plant Disease ◽  
2006 ◽  
Vol 90 (8) ◽  
pp. 1026-1030 ◽  
Author(s):  
F. Trevisan ◽  
B. M. J. Mendes ◽  
S. C. Maciel ◽  
M. L. C. Vieira ◽  
L. M. M. Meletti ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
In Vitro Selection ◽  
Passion Fruit ◽  
Cp Gene ◽  
Virus Coat ◽  
Passion Fruit Woodiness Virus ◽  
Leaf Disks ◽  
Yellow Passion Fruit

We report the use of the coat protein (CP) gene from Passion fruit woodiness virus (PWV) to produce resistant transgenic plants of yellow passion fruit. A full-length CP gene from a severe PWV isolate from the state of São Paulo, Brazil (PWV-SP) was cloned into pCAMBIA 2300 binary vector, which was further introduced into Agrobacterium tumefaciens strain EHA 105. Leaf disks were used as explants for transformation assays, e.g., 2,700 and 2,730 disks excised from plants from the Brazilian cultivars IAC-275 and IAC-277, respectively. In vitro selection was performed in kanamycin. After transferring to the elongation medium, 119 and 109 plantlets of IAC-275 and IAC-277, respectively, were recovered. Integration of the PWV CP gene was confirmed in seven of eight plants evaluated by Southern blot analysis, showing different numbers of insertional events for the CP gene. Three transgenic plants (T3, T4, and T7) expressed the expected transcript, but the 32 kDa PWV CP was detected by Western blot in only two plants (T3 and T4). The results of three successive mechanical inoculations against the transgenic plants using three PWV isolates showed that the primary transformant T2 of IAC-277 was immune to all isolates.

scholarly journals In vitro induction of autotetraploids from diploid yellow passion fruit mediated by colchicine and oryzalin

2011 ◽  
Vol 107 (3) ◽  
pp. 451-459 ◽  
Author(s):  
M. M. Rêgo ◽  
E. R. Rêgo ◽  
C. H. Bruckner ◽  
F. L. Finger ◽  
W. C. Otoni
Keyword(s):  
Passion Fruit ◽  
In Vitro Induction ◽  
Yellow Passion Fruit

scholarly journals Evaluation of postharvest properties in different passion fruit species during ripening

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Francielly Rodrigues Gomes ◽  
Moab Acacio Barbosa ◽  
Claudia Dayane Marques Rodrigues ◽  
Alejandro Hurtado Salazar ◽  
Americo Nunes da Silveira-Neto ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Passion Fruit ◽  
Soluble Solids ◽  
Chemical Characteristics ◽  
Soluble Solids Content ◽  
Post Harvest ◽  
Fruit Species ◽  
Solids Content ◽  
Days After Anthesis ◽  
Yellow Passion Fruit

During ripening, many transformations occur in passion fruits. The ripening stage affects fruit quality and post-harvest properties. Fruits with 65% yellow epidermis present chemical characteristics that meet industrial standards, facilitating harvest estimations and loss avoidance. Thus, this study aimed to evaluate the post-harvest properties of passion fruit species (genotypes of the yellow passion fruit and sweet passion fruit, and yellow passion fruit cultivar FB 200) during ripening. The fruits were evaluated by the color of the epidermis and the chemical characteristics of the pulp (titratable acidity, vitamin C, carotenoids, soluble solids content, and ratio). This experiment was conducted with a completely randomized design and a split-plot arrangement, where the plots were the species and the subplots were the evaluation periods (50, 58, 66, 74 and 82 days after anthesis), with four replications and 10 fruits per plot. The yellow passion fruit genotype and cultivar, harvested at 82 days after anthesis, presented desirable characteristics for the juice industry and for fresh consumption. The sweet passion fruit had a higher soluble solids content and titratable acidity ratio because of its high content of soluble solids and low acidity, which translate to a better fruit flavor.

Effect of different carbon sources on in vitro propagation of yellow passion fruit

2019 ◽  
pp. 389-394
Author(s):  
S.I. Costa ◽  
P.C. Mello-Farias ◽  
A.S. Copatti ◽  
I.C. Albuquerque
Keyword(s):  
In Vitro Propagation ◽  
Carbon Sources ◽  
Passion Fruit ◽  
Yellow Passion Fruit

scholarly journals Mitigating Aflatoxin Contamination in Groundnut through A Combination of Genetic Resistance and Post-Harvest Management Practices

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 315 ◽  
Author(s):  
Manish K. Pandey ◽  
Rakesh Kumar ◽  
Arun K. Pandey ◽  
Pooja Soni ◽  
Sunil S. Gangurde ◽  
...  
Keyword(s):  
Management Practices ◽  
Genetic Resistance ◽  
Aflatoxin Production ◽  
Climatic Conditions ◽  
Aflatoxin Contamination ◽  
Harvest Management ◽  
Stunted Growth ◽  
Post Harvest ◽  
Crop Varieties

Aflatoxin is considered a “hidden poison” due to its slow and adverse effect on various biological pathways in humans, particularly among children, in whom it leads to delayed development, stunted growth, liver damage, and liver cancer. Unfortunately, the unpredictable behavior of the fungus as well as climatic conditions pose serious challenges in precise phenotyping, genetic prediction and genetic improvement, leaving the complete onus of preventing aflatoxin contamination in crops on post-harvest management. Equipping popular crop varieties with genetic resistance to aflatoxin is key to effective lowering of infection in farmer’s fields. A combination of genetic resistance for in vitro seed colonization (IVSC), pre-harvest aflatoxin contamination (PAC) and aflatoxin production together with pre- and post-harvest management may provide a sustainable solution to aflatoxin contamination. In this context, modern “omics” approaches, including next-generation genomics technologies, can provide improved and decisive information and genetic solutions. Preventing contamination will not only drastically boost the consumption and trade of the crops and products across nations/regions, but more importantly, stave off deleterious health problems among consumers across the globe.

scholarly journals In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt

2011 ◽  
Vol 108 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Patrícia Silva Flores ◽  
Wagner Campos Otoni ◽  
Onkar Dev Dhingra ◽  
Sérgio Paulo Severo de Souza Diniz ◽  
Telma Miranda dos Santos ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Passion Fruit ◽  
Vascular Wilt ◽  
Yellow Passion Fruit ◽  
Selection Of
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