Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation prior to MALDI-MS Analysis

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot4610-pdb.prot4610
Author(s):  
H. Steen ◽  
A. Stensballe ◽  
O. N. Jensen
Keyword(s):  
Alkaline Phosphatase ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Phosphatase Treatment

Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation after MALDI-MS Analysis

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot4611-pdb.prot4611
Author(s):  
H. Steen ◽  
A. Stensballe ◽  
O. N. Jensen
Keyword(s):  
Alkaline Phosphatase ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Phosphatase Treatment

Alkaline Phosphatase Treatment of Phosphopeptides: On-Probe Dephosphorylation after MALDI-MS Analysis

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot4612-pdb.prot4612
Author(s):  
H. Steen ◽  
A. Stensballe ◽  
O. N. Jensen
Keyword(s):  
Alkaline Phosphatase ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Phosphatase Treatment

Proteome profile of zebrafish caudal fin based on one-dimensional gel electrophoresis LCMS/MS and two-dimensional gel electrophoresis MALDI MS/MS analysis

2010 ◽  
Vol 34 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Sachin K. Singh ◽  
Mula G. Meena Lakshmi ◽  
Sandeep Saxena ◽  
Cherukuvada V. Brahmendra Swamy ◽  
Mohammed M. Idris
Keyword(s):  
Gel Electrophoresis ◽  
Maldi Ms ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Caudal Fin ◽  
One Dimensional ◽  
Ms Analysis ◽  
Proteome Profile

Novel co-matrix systems for the MALDI-MS analysis of polystyrene using a UV absorber and stabilizer

The Analyst ◽  
2013 ◽  
Vol 138 (4) ◽  
pp. 1256 ◽  
Author(s):  
Sung-Seen Choi ◽  
Hye-Seung Chung
Keyword(s):  
Maldi Ms ◽  
Uv Absorber ◽  
Ms Analysis

scholarly journals Peptide/Protein Separation with Cationic Polymer Brush Nanosponges for MALDI-MS Analysis

Langmuir ◽  
2012 ◽  
Vol 29 (2) ◽  
pp. 696-700 ◽  
Author(s):  
Bojan Mitrovic ◽  
Stephanie Eastwood ◽  
VenNey Wong ◽  
Daniel Dyer ◽  
Gary Kinsel ◽  
...  
Keyword(s):  
Protein Separation ◽  
Polymer Brush ◽  
Cationic Polymer ◽  
Maldi Ms ◽  
Ms Analysis

scholarly journals Protein kinase C phosphorylation of rat liver S-adenosylmethionine synthetase: dissociation and production of an active monomer

1994 ◽  
Vol 303 (3) ◽  
pp. 949-955 ◽  
Author(s):  
M A Pajares ◽  
C Durán ◽  
F Corrales ◽  
J M Mato
Keyword(s):  
Alkaline Phosphatase ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Liver ◽  
Oligomeric State ◽  
Kinase C ◽  
Phosphatase Treatment ◽  
Key Enzyme ◽  
Adenosylmethionine Synthetase ◽  
Adomet Synthetase

The regulation of rat liver S-adenosylmethionine synthetase (AdoMet synthetase), a key enzyme in methionine metabolism, by protein kinase C (PKC) phosphorylation has been studied. Both enzyme forms, tetramer and dimer, are phosphorylated by this kinase in the same residue, Thr-342, of the sequence. Phosphorylation of the dimer leads to its dissociation, with production of a fully-active monomer. The kinetics of the monomer have been studied, and a KmMet of 931.9 microM, a KmATP of 708 microM and a Vmax of 66.8 nmol/min/mg have been calculated. Alkaline phosphatase treatment of both enzyme forms (tetramer and dimer) produces a reduction in their activity with no change in the oligomeric state. On the other hand, PKC phosphorylation of the alkaline phosphatase-treated AdoMet synthetase forms leads to the dissociation of the dimer to produce a monomer. Rephosphorylation occurs again in the same residue, Thr-342, of the sequence. The significance of AdoMet synthetase regulation by PKC phosphorylation is further discussed.

MALDI-MS Analysis of Sucrose Using a Charcoal Matrix with Different Cationization Agents

2018 ◽  
Vol 39 (6) ◽  
pp. 750-756 ◽  
Author(s):  
Jihyun Paek ◽  
Yeoseon Kim ◽  
Dabin Lee ◽  
Jeongkwon Kim
Keyword(s):  
Maldi Ms ◽  
Ms Analysis

Platelet Factor 4 as a Novel Exosome Marker in MALDI-MS Analysis of Exosomes from Human Serum

2019 ◽  
Vol 91 (20) ◽  
pp. 13297-13305 ◽  
Author(s):  
Huu-Quang Nguyen ◽  
Dabin Lee ◽  
Yeoseon Kim ◽  
Minseok Paek ◽  
Minsun Kim ◽  
...  
Keyword(s):  
Human Serum ◽  
Platelet Factor 4 ◽  
Maldi Ms ◽  
Platelet Factor ◽  
Ms Analysis
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