Quantitative Electron Microscopy of Chromosome Organization at Meiotic Prophase

1974 ◽  
Vol 38 (0) ◽  
pp. 99-107 ◽  
Author(s):  
P. B. Moens
Keyword(s):  
Electron Microscopy ◽  
Meiotic Prophase ◽  
Chromosome Organization ◽  
Quantitative Electron Microscopy

scholarly journals DNA AND THE FINE STRUCTURE OF SYNAPTIC CHROMOSOMES IN THE DOMESTIC ROOSTER (GALLUS DOMESTICUS)

1964 ◽  
Vol 23 (1) ◽  
pp. 63-78 ◽  
Author(s):  
James R. Coleman ◽  
Montrose J. Moses
Keyword(s):  
Electron Microscopy ◽  
Heavy Metal ◽  
Fine Structure ◽  
Electron Microscope ◽  
Meiotic Prophase ◽  
Gallus Domesticus ◽  
Feulgen Staining ◽  
Synaptinemal Complex ◽  
Relationship Of ◽  
The Relationship

The indium trichloride method of Watson and Aldridge (38) for staining nucleic acids for electron microscopy was employed to study the relationship of DNA to the structure of the synaptinemal complex in meiotic prophase chromosomes of the domestic rooster. The selectivity of the method was demonstrated in untreated and DNase-digested testis material by comparing the distribution of indium staining in the electron microscope to Feulgen staining and ultraviolet absorption in thicker sections seen with the light microscope. Following staining by indium, DNA was found mainly in the microfibril component of the synaptinemal complex. When DNA was known to have been removed from aldehyde-fixed material by digestion with DNase, indium stainability was also lost. However, staining of the digested material with non-selective heavy metal techniques demonstrated the presence of material other than DNA in the microfibrils and showed that little alteration in appearance of the chromosome resulted from DNA removal. The two dense lateral axial elements of the synaptinemal complex, but not the central one to any extent, also contained DNA, together with non-DNA material.

scholarly journals Basophilic Lamellar Systems in the Crayfish Spermatocyte

1958 ◽  
Vol 4 (3) ◽  
pp. 267-274 ◽  
Author(s):  
August Ruthmann
Keyword(s):  
Electron Microscopy ◽  
Small Rna ◽  
Meiotic Prophase ◽  
Lamellar Body ◽  
Annulate Lamellae ◽  
Late Prophase ◽  
Lamellar System ◽  
Thin Sections ◽  
Early Spermatid ◽  
Break Up

Histochemical procedures for the demonstration of RNA have shown the presence of intensely basophilic bodies in the cytoplasm of spermatocytes of the crayfish, Cambarus virilis. The staining of thick sections, cut alternately with thin sections for electron microscopy, has permitted identification of the basophilic bodies with two types of lamellar systems. One of these, a set of straight annulate lamellae, is restricted to meiotic prophase. The second type of lamellar systems has been found from late prophase to early spermatid stages. It consists of an ellipsoidal lamellar set which intersects a number of straight lamellae. Within the region of intersection, the ellipsoidal lamellae break up into an array of small tubules of about 150 A diameter. The term tubulate lamellar system was chosen to designate this type of lamellar complex. Small RNA-containing granules could not be detected in annulate lamellar systems. While there are a few granules in the marginal regions of the tubulate lamellar system, their distribution cannot be responsible for the basophilia which is intense within all regions of the lamellar body.

Analysis of human chromosomal variants by quantitative electron microscopy

Chromosoma ◽  
1971 ◽  
Vol 34 (1) ◽  
pp. 94-98 ◽  
Author(s):  
H. M. Grolomb ◽  
G. F. Bahr ◽  
D. S. Borgaonkar
Keyword(s):  
Electron Microscopy ◽  
Quantitative Electron Microscopy ◽  
Chromosomal Variants

Transient Nucleation in Co-Zr Metallic Glasses

1985 ◽  
Vol 57 ◽  
Author(s):  
Uwe Köster ◽  
Margret Blank-Bewersdorff
Keyword(s):  
Electron Microscopy ◽  
Metallic Glasses ◽  
Crystal Size ◽  
Time Lag ◽  
Size Distributions ◽  
Quantitative Electron Microscopy ◽  
Crystal Size Distributions ◽  
Different Temperatures ◽  
Transient Nucleation ◽  
Good Agreement

AbstractCrystallization kinetics and crystal size distributions in Co33Zr67-glasses have been analyzed by quantitative electron microscopy. The polymorphic crystallization of spherical CoZr2 crystals is very suitable reaction for such an analysis. Calculated crystal size distributions at different temperatures were compared to those experimentally revealed. Parameters controlling crystallization were varied within reasonable limits until theoretically calculated and experimentally observed crystal size distributions were in good agreement. It has been found that crystal size distribution can be explained by transient nucleation; the time lag and its temperature dependence can be evaluated. These results are discussed in the light of recent theories on transient nucleation.

Using quantitative electron microscopy for process mineralogy applications

JOM ◽  
2000 ◽  
Vol 52 (4) ◽  
pp. 24-25 ◽  
Author(s):  
P. Gottlieb ◽  
G. Wilkie ◽  
D. Sutherland ◽  
E. Ho-Tun ◽  
S. Suthers ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Quantitative Electron Microscopy ◽  
Process Mineralogy

Quantitative electron microscopy of FePt nano-crystallites in amorphous Al2O3 matrix

2003 ◽  
Vol 48 (7) ◽  
pp. 915-920 ◽  
Author(s):  
K. Kaneko ◽  
S. Matsumura ◽  
K. Ikematsu ◽  
Y. Kato ◽  
Y. Tomokiyo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Quantitative Electron Microscopy ◽  
Amorphous Al2o3 ◽  
Al2o3 Matrix

scholarly journals STUDY OF MITOCHONDRIA IN RAT LIVER

1962 ◽  
Vol 15 (3) ◽  
pp. 489-501 ◽  
Author(s):  
Gunter F. Bahr ◽  
Elmar Zeitler
Keyword(s):  
Electron Microscopy ◽  
Rat Liver ◽  
Linear Growth ◽  
Mitochondrial Fraction ◽  
The Other ◽  
Quantitative Electron Microscopy ◽  
Normal Rat ◽  
Log Normal ◽  
Log Normal Distribution ◽  
Particle Weight

The electron microscope has been used to determine the weight distribution of isolated subcellular particles from normal rat liver. The following results are reported: (1) There exist at least two well defined weight populations of subcellular particles; their respective median weights are 1.3 x 10-14 and 11 x 10-14 gm. The lighter fraction is considered to consist of lysosomes, the heavier of mitochondria. (2) The mitochondrial fraction shows a log-normal distribution of the particle weight. (3) By the introduction of morphologic criteria, the mitochondrial fraction is divided into two groups, one consisting of a spherical, the other of an oblong type of particle. The data found support the following concepts: (a) Mitochondria increase their weight from a certain size up by linear growth. (b) Mitochondria divide. The division is not necessarily symmetric; in all cases, however, one part of the division product is a spherical particle. It is felt that these results constitute a valuable demonstration of the general capabilities of quantitative electron microscopy and may stimulate many other useful applications of this technique in cytology, bacteriology, and virology.

scholarly journals Quantitative electron microscopy

Nature ◽  
1980 ◽  
Vol 283 (5747) ◽  
pp. 608-608
Author(s):  
V.E. Cosslett
Keyword(s):  
Electron Microscopy ◽  
Quantitative Electron Microscopy
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