Initiation of Replication of Plasmid ColE1 DNA by RNA Polymerase, Ribonuclease H, and DNA Polymerase I

1979 ◽  
Vol 43 (0) ◽  
pp. 409-417 ◽  
Author(s):  
T. Itoh ◽  
J. Tomizawa
Keyword(s):  
Rna Polymerase ◽  
Dna Polymerase ◽  
Ribonuclease H ◽  
Dna Polymerase I ◽  
Initiation Of Replication ◽  
Polymerase I

scholarly journals Genetic evidence for two protein domains and a potential new activity in bacteriophage T4 DNA polymerase.

Genetics ◽  
1990 ◽  
Vol 124 (2) ◽  
pp. 213-220 ◽  
Author(s):  
L J Reha-Krantz
Keyword(s):  
Dna Polymerase ◽  
Bacteriophage T4 ◽  
Rnase H ◽  
Ribonuclease H ◽  
Temperature Sensitive ◽  
Polymerase Gene ◽  
Dna Polymerase I ◽  
E Coli ◽  
Intragenic Complementation ◽  
Polymerase I

Abstract Intragenic complementation was detected within the bacteriophage T4 DNA polymerase gene. Complementation was observed between specific amino (N)-terminal, temperature-sensitive (ts) mutator mutants and more carboxy (C)-terminal mutants lacking DNA polymerase polymerizing functions. Protein sequences surrounding N-terminal mutation sites are similar to sequences found in Escherichia coli ribonuclease H (RNase H) and in the 5'----3' exonuclease domain of E. coli DNA polymerase I. These observations suggest that T4 DNA polymerase, like E. coli DNA polymerase I, contains a discrete N-terminal domain.

Viroid RNA is accepted as a template for in vitro transcription by DNA-dependent DNA polymerase I and RNA polymerase from Escherichia coli

1982 ◽  
Vol 2 (11) ◽  
pp. 929-939 ◽  
Author(s):  
Wolfgang Rohde ◽  
Hans-Richard Rackwitz ◽  
Frank Boege ◽  
Heinz L. Sänger
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Dna Polymerase ◽  
Unit Length ◽  
Dna Polymerase I ◽  
Experimental Conditions ◽  
In Vitro Synthesis ◽  
Polymerase I

The RNA genome of potato spindle tuber viroid (PSTV) is transcribed in vitro into complementary DNA and RNA by DNA-dependent DNA polymerase I and RNA polymerase, respectively, from Escherichia coli. In vitro synthesis of complementary RNA produces distinct transcripts larger than unit length thus reflecting the in vivo mechanism of viroid replication. The influence of varying experimental conditions on the transcription process is studied; actinomycin D is found to drastically reduce complementary RNA synthesis from the PSTV RNA template by RNA polymerase.

Molecular Modeling of RNA Polymerase II Mutations onto DNA Polymerase I

1994 ◽  
Vol 244 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Wan Joon Kim ◽  
Lillian P. Burke ◽  
Mark A. Mortin
Keyword(s):  
Molecular Modeling ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Polymerase I

scholarly journals Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Ani Saghatelyan ◽  
Hovik Panosyan ◽  
Armen Trchounian ◽  
Nils‐Kåre Birkeland
Keyword(s):  
Dna Polymerase ◽  
Extreme Thermophile ◽  
Dna Polymerase I ◽  
Polymerase I
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