Growth-related Alterations Induced in Chick Embryo Fibroblasts by src-gene Deletion Mutants of the Schmidt-Ruppin Strain of Rous Sarcoma Virus

1980 ◽  
Vol 44 (0) ◽  
pp. 1047-1055 ◽  
Author(s):  
M. L. Breitman ◽  
R. Vigne ◽  
P. K. Vogt
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Gene Deletion ◽  
Deletion Mutants ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts

scholarly journals Decreased levels of collagen mRNA in rous sarcoma virus-transformed chick embryo fibroblasts

1978 ◽  
Vol 253 (16) ◽  
pp. 5869-5874
Author(s):  
B.H. Howard ◽  
S.L. Adams ◽  
M.E. Sobel ◽  
I. Pastan ◽  
B. de Crombrugghe
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Collagen Mrna ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts

Long-term cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain (D) of Rous sarcoma virus

1976 ◽  
Vol 22 (10) ◽  
pp. 1474-1479
Author(s):  
Lorraine Leblond-Larouche ◽  
Réjean Morais
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Viable Cells ◽  
Deficient Medium ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Roller Cultures

Attempts have been made to keep in vitro, for extended periods of time, cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain of Rous sarcoma virus, subgroup D. Roller cultures of transformed chick cells kept in serum-deficient medium can be maintained without subcultivation for up to 6 months. The confluent cultures continuously release viruses and viable tumor cells into the medium. The released cells can be plated and have characteristics of growth and morphology which are relatively stable with time until the culture degenerates. Cells released at later stages of the culture produced substantially more viruses than those released earlier, suggesting that cell selection or differentiation occurs during long-term cultivation in low serum concentration. Long-term cultures of untransformed chick embryo fibroblasts can also be maintained in the same way. The release of viable cells by these confluent cultures, however, is negligible.

scholarly journals Expression of the v-src or v-fps oncogene increases fructose 2,6-bisphosphate in chick-embryo fibroblasts. Novel mechanism for the stimulation of glycolysis by retroviruses

1986 ◽  
Vol 236 (2) ◽  
pp. 595-599 ◽  
Author(s):  
L Bosca ◽  
M Mojena ◽  
J Ghysdael ◽  
G G Rousseau ◽  
L Hue
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Kinase Activation ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Rate Limiting ◽  
Stimulation Of

The concentration of fructose 2,6-bisphosphate and the activity of 6-phosphofructo-2-kinase are increased after infection of chick-embryo fibroblasts with the Rous sarcoma virus, or with a temperature-sensitive mutant of this virus at the permissive, but not at the non-permissive, temperature. This is observed after transformation by retroviruses carrying either the v-src or v-fps, but not the v-mil and/or v-myc, oncogenes. Comparison of the effects of the Rous sarcoma virus with those of phorbol myristate acetate on fructose 2,6-bisphosphate suggests that both result from the stimulation of a step which is rate-limiting for 6-phosphofructo-2-kinase activation and which is also controlled by protein kinase C.

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